Solution NMR structure of human Rev1 (932-1039) in complex with ubiquitin
GHMPSPSQLD QSVLEALPPD LREQVEQVCA VQQAESHGDK KKEPVNGCNT GILPQPVGTV LLQIPEPQES NSDAGINLIA LPAFSQVDPE VFAALPAELQ RELKAAYDQR Q
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 2:CYS29:SG | 2:CYS48:SG |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 94.4 % (2043 of 2165) | 93.3 % (1062 of 1138) | 97.0 % (805 of 830) | 89.3 % (176 of 197) |
Backbone | 96.9 % (1058 of 1092) | 96.5 % (358 of 371) | 97.3 % (534 of 549) | 96.5 % (166 of 172) |
Sidechain | 92.7 % (1157 of 1248) | 91.8 % (704 of 767) | 97.1 % (443 of 456) | 40.0 % (10 of 25) |
Aromatic | 82.4 % (56 of 68) | 82.4 % (28 of 34) | 82.4 % (28 of 34) | |
Methyl | 99.6 % (223 of 224) | 99.1 % (111 of 112) | 100.0 % (112 of 112) |
1. entity 1
MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG2. entity 2
GHMPSPSQLD QSVLEALPPD LREQVEQVCA VQQAESHGDK KKEPVNGCNT GILPQPVGTV LLQIPEPQES NSDAGINLIA LPAFSQVDPE VFAALPAELQ RELKAAYDQR QSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.8, Details 1 mM [U-100% 13C; U-100% 15N] Ubiquitin, 20 mM Sodium phosphate buffer, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Sodium phosphate buffer | natural abundance | 20 mM | |
2 | Ubiquitin | [U-100% 13C; U-100% 15N] | 1 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.8, Details 1 mM [U-100% 13C; U-100% 15N] Rev1, 3 mM Ubiquitin, 50 mM Sodium phosphate buffer, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
3 | Rev1 | [U-100% 13C; U-100% 15N] | 1 mM | |
4 | Sodium phosphate buffer | natural abundance | 50 mM | |
5 | Ubiquitin | natural abundance | 3 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.8, Details 3 mM Rev1, 1 mM [U-100% 13C; U-100% 15N] Ubiquitin, 50 mM Sodium phosphate buffer, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | Rev1 | natural abundance | 3 mM | |
7 | Sodium phosphate buffer | natural abundance | 50 mM | |
8 | Ubiquitin | [U-100% 13C; U-100% 15N] | 1 mM |