BMRB: 30327

NMR solution structure of Rtt103 (RTT) protein using two 4D-spectra

Authors

Evangelidis, T., Nerli, S., Sgourakis, N.G., Tripsianes, K.

Assembly

Regulator of Ty1 transposition protein 103

Entity

1. Regulator of Ty1 transposition protein 103 (polymer), 138 monomers, 16103.33 Da Detail

SEQFTTKLNT LEDSQESISS ASKWLLLQYR DAPKVAEMWK EYMLRPSVNT RRKLLGLYLM NHVVQQAKGQ KIIQFQDSFG KVAAEVLGRI NQEFPRDLKK KLSRVVNILK ERNIFSKQVV NDIERSLAAA LEHHHHHH

Formula weight

16103.33 Da

Source organism

Saccharomyces cerevisiae S288C

Exptl. method

solution NMR

Refine. method

na

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 96.4 %, Completeness: 84.7 %, Completeness (bb): 82.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	84.7 % (1442 of 1703)	90.4 % (810 of 896)	74.7 % (488 of 653)	93.5 % (144 of 154)
Backbone	82.6 % (679 of 822)	94.6 % (262 of 277)	70.7 % (290 of 410)	94.1 % (127 of 135)
Sidechain	87.9 % (892 of 1015)	88.5 % (548 of 619)	86.7 % (327 of 377)	89.5 % (17 of 19)
Aromatic	27.0 % (34 of 126)	27.0 % (17 of 63)	27.9 % (17 of 61)	0.0 % (0 of 2)
Methyl	99.4 % (157 of 158)	98.7 % (78 of 79)	100.0 % (79 of 79)

1. Regulator of Ty1 transposition protein 103

SEQFTTKLNT LEDSQESISS ASKWLLLQYR DAPKVAEMWK EYMLRPSVNT RRKLLGLYLM NHVVQQAKGQ KIIQFQDSFG KVAAEVLGRI NQEFPRDLKK KLSRVVNILK ERNIFSKQVV NDIERSLAAA LEHHHHHH

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 0.8 mM [U-13C; U-15N] Rtt103 (RTT) protein, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	Rtt103 (RTT) protein	[U-13C; U-15N]		0.8 mM
2	Potassium Phosphate	natural abundance		35 mM
3	KCl	natural abundance		100 mM

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LACS Plot; CA

Referencing offset: 0.01 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.01 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.1 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

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Protein Blocks Logo

Calculated from 10 models in PDB: 5WOZ, Strand ID: A Detail

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Release date

2017-08-29

Citation

Automated NMR resonance assignments and structure determination using a minimal set of 4D spectra
Evangelidis, T., Nerli, S., Novacek, J., Brereton, A.E., Karplus, P.A., Dotas, R.R., Venditti, V., Sgourakis, N.G., Tripsianes, K.
Nat. Commun. (2018), 9, 384-384, PubMed 29374165 , DOI 10.1038/s41467-017-02592-z , Abstract

Related entities 1. Regulator of Ty1 transposition protein 103, : 1 : 4 : 2 : 58 entities Detail

Interaction partners 1. Regulator of Ty1 transposition protein 103, : 33 interactors Detail

Experiments performed 3 experiments Detail