BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	90.3 % (1224 of 1355)	90.7 % (643 of 709)	89.6 % (476 of 531)	91.3 % (105 of 115)
Backbone	92.6 % (626 of 676)	91.8 % (212 of 231)	93.8 % (316 of 337)	90.7 % (98 of 108)
Sidechain	88.7 % (697 of 786)	90.2 % (431 of 478)	86.4 % (260 of 301)	85.7 % (6 of 7)
Aromatic	62.1 % (87 of 140)	70.0 % (49 of 70)	53.6 % (37 of 69)	100.0 % (1 of 1)
Methyl	100.0 % (92 of 92)	100.0 % (46 of 46)	100.0 % (46 of 46)

1. entity 1

MHHHHHHSTS VDLGTENLYF QSNAGKMFIG GLSWQTSPDS LRDYFSKFGE IRECMVMRDP TTKRSRGFGF VTFADPASVD KVLGQPHHEL DSKTIDPKVA FPRRAQPKMV TRTKK

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 0.5 mM [U-90% 15N] MSI2-RRM1, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	MSI2-RRM1	[U-90% 15N]		0.5 mM
2	NaCl	natural abundance		150 mM

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 0.7 mM [U-95% 13C; U-90% 15N] MSI2-RRM1, 0.6 mM [U-95% 13C; U-90% 15N] MSI2-RRM1, 1 mM [U-95% 13C; U-90% 15N] MSI2-RRM1, 0.4 mM [U-95% 13C; U-90% 15N] MSI2-RRM1, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
3	MSI2-RRM1	[U-95% 13C; U-90% 15N]		0.7 mM
4	NaCl	natural abundance		150 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.16 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.16 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.23 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 6C8U, Strand ID: 1 Detail

Release date

2018-08-14

Citation

Crystal and solution structures of human oncoprotein Musashi-2 N-terminal RNA recognition motif 1
Lan, L., Xing, M., Kashipathy, M., Douglas, J., Gao, P., Battaile, K., Hanzlik, R., Lovell, S., Xu, L.
Proteins (2020), 88, 573-583, PubMed 31603583 , DOI 10.1002/prot.25836 , Abstract

Related entities 1. RNA-binding protein Musashi homolog 2, : 25 entities Detail

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 0.5 mM [U-90% 15N] MSI2-RRM1, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	MSI2-RRM1	[U-90% 15N]		0.5 mM
2	NaCl	natural abundance		150 mM

2 3D CBCA(CO)NH

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 0.7 mM [U-95% 13C; U-90% 15N] MSI2-RRM1, 0.6 mM [U-95% 13C; U-90% 15N] MSI2-RRM1, 1 mM [U-95% 13C; U-90% 15N] MSI2-RRM1, 0.4 mM [U-95% 13C; U-90% 15N] MSI2-RRM1, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
3	MSI2-RRM1	[U-95% 13C; U-90% 15N]		0.7 mM
4	NaCl	natural abundance		150 mM

3 3D HCCH-TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
3	MSI2-RRM1	[U-95% 13C; U-90% 15N]		0.7 mM
4	NaCl	natural abundance		150 mM

4 3D HNCACB

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
3	MSI2-RRM1	[U-95% 13C; U-90% 15N]		0.7 mM
4	NaCl	natural abundance		150 mM

5 3D HNCA

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
3	MSI2-RRM1	[U-95% 13C; U-90% 15N]		0.7 mM
4	NaCl	natural abundance		150 mM

6 3D HNCO

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
3	MSI2-RRM1	[U-95% 13C; U-90% 15N]		0.7 mM
4	NaCl	natural abundance		150 mM

7 3D HN(CO)CA

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
3	MSI2-RRM1	[U-95% 13C; U-90% 15N]		0.7 mM
4	NaCl	natural abundance		150 mM

8 3D HNCACO

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
3	MSI2-RRM1	[U-95% 13C; U-90% 15N]		0.7 mM
4	NaCl	natural abundance		150 mM

9 3D 1H-15N NOESY

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
3	MSI2-RRM1	[U-95% 13C; U-90% 15N]		0.7 mM
4	NaCl	natural abundance		150 mM

10 3D 1H-13C NOESY

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
3	MSI2-RRM1	[U-95% 13C; U-90% 15N]		0.7 mM
4	NaCl	natural abundance		150 mM

11 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 0.5 mM [U-90% 15N] MSI2-RRM1, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	MSI2-RRM1	[U-90% 15N]		0.5 mM
2	NaCl	natural abundance		150 mM

12 3D HCCH-COSY

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
3	MSI2-RRM1	[U-95% 13C; U-90% 15N]		0.7 mM
4	NaCl	natural abundance		150 mM

NMR combined restraints 6 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints	combined_30398_6c8u.nef
Input source #2: Coordindates	6c8u.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): 1 vs Auth_asym_ID (model): 1

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
1	1	115	0	0	100.0

Content subtype: combined_30398_6c8u.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	1226		93.9 (chain: 1, length: 115)
1	Distance restraints	CNS/XPLOR_distance_constraints_5	OK	977 (1)	noe	91.3 (chain: 1, length: 115)
2	Distance restraints	CNS/XPLOR_distance_constraints_7	OK	639 (1)	noe	78.3 (chain: 1, length: 115)
3	Distance restraints	CNS/XPLOR_distance_constraints_4	Warning	38 (1)	hbond	27.8 (chain: 1, length: 115)
1	Dihedral angle restraints	CNS/XPLOR_dihedral_6	OK	148 (148)	.	73.0 (chain: 1, length: 115)

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 115, Sequence coverage: 93.9%
- Total number of assignments
  - ¹H chemical shifts: 642
  - ¹³C chemical shifts: 477
  - ¹⁵N chemical shifts: 107
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
13	ASN	CG	176.73
19	ASN	CG	176.58
31	GLN	CD	180.25
81	GLN	CD	180.44
102	GLN	CD	180.46

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	709	642	90.6
¹³C chemical shifts	531	472	88.9
¹⁵N chemical shifts	123	107	87.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	231	214	92.6
¹³C chemical shifts	230	216	93.9
¹⁵N chemical shifts	108	98	90.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	478	428	89.5
¹³C chemical shifts	301	256	85.0
¹⁵N chemical shifts	15	9	60.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	51	50	98.0
¹³C chemical shifts	51	50	98.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	70	45	64.3
¹³C chemical shifts	69	33	47.8
¹⁵N chemical shifts	1	1	100.0

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_5
- Status: OK
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 115, Sequence coverage: 91.3%
- Total number of restraints: 975
- Weight of restraints: 1.0 (975)
- Potential type of restraints: square-well-parabolic (975)
- Range of distance target values: 2.1, 3.75 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: 1
- Distance restraints on contact map
- Saveframe: CNS/XPLOR_distance_constraints_7
  - Status: OK
  - Experiment type: noe
  - Sequence coverage of experimental data
    - Entity_assembly_ID: 1, Chain length: 115, Sequence coverage: 78.3%
  - Total number of restraints: 637
  - Weight of restraints: 1.0 (637)
  - Potential type of restraints: square-well-parabolic (637)
  - Range of distance target values: 2.1, 3.5 (Å)
  - Histogram of distance target values
  - Histogram of discrepancy in distance target values
  - Distance restraints per residue
    - Chain_ID: 1
  - Distance restraints on contact map
  - Saveframe: CNS/XPLOR_distance_constraints_4
    - Status: Warning
    - Experiment type: hbond
    - Sequence coverage of experimental data
      - Entity_assembly_ID: 1, Chain length: 115, Sequence coverage: 27.8%
    - Total number of restraints: 38
    - Weight of restraints: 1.0 (38)
    - Potential type of restraints: square-well-parabolic (38)
    - Range of distance target values: 2.05, 2.95 (Å)
    - Histogram of distance target values
    - Distance restraints per residue
      - Chain_ID: 1
    - Distance restraints on contact map

Dihedral angle restraints

Saveframe: CNS/XPLOR_dihedral_6
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 115, Sequence coverage: 73.0%
- Total number of restraints: 148
- Total number of restraints per polymer type: 148
- Weight of restraints: 1.0 (148)
- Potential type of restraints: square-well-parabolic (148)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: 1

Keywords RNA binding protein, RNA recognition motif

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (0.627 seconds)

BMRB: 30398

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. RNA-binding protein Musashi homolog 2, : 25 entities Detail

Experiments performed 12 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

NMR combined restraints 6 contents Detail