BMRBj - BMREntryMaster

Found 1 Document (2.47 seconds)

BMRB: 30404

6CE5

NMR structure of the Rous sarcoma virus matrix protein (M-domain) in the presence of myo-inositol hexakisphosphate

Authors

Vlach, J., Saad, J.S.

Assembly

Gag-Pol polyprotein (E.C.2.7.7.-,3.1.-.-)

Entity

1. Gag-Pol polyprotein (E.C.2.7.7.-,3.1.-.-) (polymer, Thiol state: all free), 87 monomers, 9198.681 Da Detail

SEAVIKVISS ACKTYCGKTS PSKKEIGAML SLLQKEGLLM SPSDLYSPGS WDPITAALSQ RAMILGKSGE LKTWGLVLGA LKAAREE

Formula weight

9198.681 Da

Source organism

Rous sarcoma virus - Prague C

Exptl. method

solution NMR

Refine. method

torsion angle dynamicstorsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 98.1 %, Completeness (bb): 98.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	98.1 % (963 of 982)	97.8 % (499 of 510)	97.9 % (377 of 385)	100.0 % (87 of 87)
Backbone	98.1 % (504 of 514)	98.9 % (176 of 178)	96.8 % (245 of 253)	100.0 % (83 of 83)
Sidechain	98.2 % (537 of 547)	97.3 % (323 of 332)	99.5 % (210 of 211)	100.0 % (4 of 4)
Aromatic	100.0 % (40 of 40)	100.0 % (20 of 20)	100.0 % (18 of 18)	100.0 % (2 of 2)
Methyl	100.0 % (106 of 106)	100.0 % (53 of 53)	100.0 % (53 of 53)

1. entity 1

SEAVIKVISS ACKTYCGKTS PSKKEIGAML SLLQKEGLLM SPSDLYSPGS WDPITAALSQ RAMILGKSGE LKTWGLVLGA LKAAREE

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 305 K, pH 6, Details 0.5 mM [U-95% 15N] Matrix protein, 50 mM sodium phosphate, 2 mM tcep, 2 mM IP6, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	Matrix protein	[U-95% 15N]	0.5 mM
2	sodium phosphate	natural abundance	50 mM
3	tcep	natural abundance	2 mM
4	IP6	natural abundance	2 mM

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 305 K, pH 6, Details 0.5 mM [U-95% 13C; U-95% 15N] Matrix protein, 50 mM sodium phosphate, 2 mM tcep, 2 mM IP6, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
5	Matrix protein	[U-95% 13C; U-95% 15N]	0.5 mM
6	sodium phosphate	natural abundance	50 mM
7	tcep	natural abundance	2 mM
8	IP6	natural abundance	2 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.34 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.34 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.46 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 6CE5, Strand ID: A Detail

Release date

2018-02-20

Citation

Structural basis for targeting avian sarcoma virus Gag polyprotein to the plasma membrane for virus assembly
Vlach, J., Eastep, G.N., Ghanam, R.H., Watanabe, S.M., Carter, C.A., Saad, J.S.
J. Biol. Chem. (2018), 293, 18828-18840, PubMed 30309983 , DOI 10.1074/jbc.RA118.003944 , Abstract

Related entities 1. Gag-Pol polyprotein (E.C.2.7.7.-,3.1.-.-), : 1 : 4 : 6 : 11 entities Detail

Interaction partners 1. Gag-Pol polyprotein (E.C.2.7.7.-,3.1.-.-), : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 5 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AvanceII - 700 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 305 K, pH 6, Details 0.5 mM [U-95% 15N] Matrix protein, 50 mM sodium phosphate, 2 mM tcep, 2 mM IP6, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	Matrix protein	[U-95% 15N]	0.5 mM
2	sodium phosphate	natural abundance	50 mM
3	tcep	natural abundance	2 mM
4	IP6	natural abundance	2 mM

2 2D 1H-13C HSQC

Instrument

Bruker AvanceII - 700 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 305 K, pH 6, Details 0.5 mM [U-95% 13C; U-95% 15N] Matrix protein, 50 mM sodium phosphate, 2 mM tcep, 2 mM IP6, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
5	Matrix protein	[U-95% 13C; U-95% 15N]	0.5 mM
6	sodium phosphate	natural abundance	50 mM
7	tcep	natural abundance	2 mM
8	IP6	natural abundance	2 mM

3 3D HNCA

Instrument

Bruker AvanceII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
5	Matrix protein	[U-95% 13C; U-95% 15N]	0.5 mM
6	sodium phosphate	natural abundance	50 mM
7	tcep	natural abundance	2 mM
8	IP6	natural abundance	2 mM

4 3D HN(CO)CA

Instrument

Bruker AvanceII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
5	Matrix protein	[U-95% 13C; U-95% 15N]	0.5 mM
6	sodium phosphate	natural abundance	50 mM
7	tcep	natural abundance	2 mM
8	IP6	natural abundance	2 mM

5 3D HNCACB

Instrument

Bruker AvanceII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
5	Matrix protein	[U-95% 13C; U-95% 15N]	0.5 mM
6	sodium phosphate	natural abundance	50 mM
7	tcep	natural abundance	2 mM
8	IP6	natural abundance	2 mM

nullKeywords ASV, Gag, IP6, RSV, VIRAL PROTEIN

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Found 1 Document (2.47 seconds)

BMRB: 30404

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Gag-Pol polyprotein (E.C.2.7.7.-,3.1.-.-), : 1 : 4 : 6 : 11 entities Detail

Interaction partners 1. Gag-Pol polyprotein (E.C.2.7.7.-,3.1.-.-), : 1 interactors Detail

Experiments performed 5 experiments Detail

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