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HRFLRH peptide NMR structure in the presence of Cd(II)
Authors
Pires, D.A.T., Arake, L.M.R., Silva, L.P., Lopez-Castillo, A., Prates, M.V., Nascimento, C.J., Bloch Jr, C.
Assembly
Hexapeptide HRFLRH
Entity
1. Hexapeptide HRFLRH (polymer, Thiol state: not present), 7 monomers, 863.0036 Da Detail

HRFLRHX


Formula weight
863.0036 Da
Source organism
Pithecopus centralis
Exptl. method
solution NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 85.7 %, Completeness: 92.5 %, Completeness (bb): 80.0 % Detail

Polymer type: polypeptide(L)

Total1H13C
All92.5 % (74 of 80)97.8 % (45 of 46)85.3 % (29 of 34)
Backbone80.0 % (24 of 30)91.7 % (11 of 12)72.2 % (13 of 18)
Sidechain100.0 % (56 of 56)100.0 % (34 of 34)100.0 % (22 of 22)
Aromatic100.0 % (18 of 18)100.0 % (9 of 9)100.0 % (9 of 9)
Methyl100.0 % (4 of 4)100.0 % (2 of 2)100.0 % (2 of 2)

1. entity 1

HRFLRHX

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 4.3, Details 2 mM HRFLRH peptide, 2 mM Cd(II), 90% H2O/10% D2O


#NameIsotope labelingTypeConcentration
1HIS-ARG-PHE-LEU-ARG-HIS[U-13C]2 mM
2Cd(II)natural abundance2 mM

Release date
2018-05-20
Citation
A previously undescribed hexapeptide His-Arg-Phe-Leu-Arg-His-NH2 from amphibian skin secretion shows CO2 and metal biding affinities
Pires, D.A.T., Arake, L.M.R., Silva, L.P., Lopez-Castillo, A., Prates, M.V., Nascimento, C.J., Bloch Jr, C.
Peptides (2018), 106, 37-44, PubMed 29933027 , DOI 10.1016/j.peptides.2018.06.003 ,
Experiments performed 5 experiments Detail
NMR combined restraints 3 contents Detail
Keywords Amphibian Skin Secretion, CO2 affinity, METAL BINDING PROTEIN, Metal Affinity