De Novo Design of a Protein Heterodimer with Specificity Mediated by Hydrogen Bond Networks
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 81.7 % (1633 of 1999) | 80.1 % (864 of 1079) | 82.8 % (623 of 752) | 86.9 % (146 of 168) |
Backbone | 88.7 % (843 of 950) | 88.4 % (283 of 320) | 89.0 % (421 of 473) | 88.5 % (139 of 157) |
Sidechain | 77.6 % (934 of 1204) | 76.5 % (581 of 759) | 79.7 % (346 of 434) | 63.6 % (7 of 11) |
Aromatic | 17.9 % (5 of 28) | 21.4 % (3 of 14) | 14.3 % (2 of 14) | |
Methyl | 82.1 % (151 of 184) | 81.5 % (75 of 92) | 82.6 % (76 of 92) |
1. entity 1
GTKEDILERQ RKIIERAQEI HRRQQEILEE LERIIRKPGS SEEAMKRMLK LLEESLRLLK ELLELSEESA QLLYEQR2. entity 2
TEKRLLEEAE RAHREQKEII KKAQELHRRL EEIVRQSGSS EEAKKEAKKI LEEIRELSKR SLELLREILY LSQEQKGSLV PRSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7.2, Details 1.0 mM [U-13C; U-15N] DHD13_XAAA, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | entity_1 | [U-13C; U-15N] | 1.0 (±0.1) mM | |
2 | entity_2 | [U-13C; U-15N] | 1.0 (±0.1) mM | |
3 | NaCl | natural abundance | 50 mM | |
4 | NaPO4 | natural abundance | 20 mM |