BMRBj - BMREntryMaster

Found 1 Document (1.173 seconds)

BMRB: 30501

6E83

Solution structure of ZZZ3 ZZ domain in complex with histone H3 tail

Authors

Zhang, Y., Kutateladze, T.G.

Assembly

ZZ-type zinc finger-containing protein 3, Histone H3

Entity

1. ZZ-type zinc finger-containing protein 3, Histone H3, entity 1 (polymer, Thiol state: free and other bound), 64 monomers, 7270.051 Da Detail

GPLGSVQHVG FKCDNCGIEP IQGVRWHCQD CPPEMSLDFC DSCSDCLHET DIHKEDHQLE PIYR

2. ZZ-type zinc finger-containing protein 3, Histone H3, entity 2 (polymer, Thiol state: not present), 12 monomers, 1304.455 Da Detail

ARTKQTARKS TG

3. ZZ-type zinc finger-containing protein 3, Histone H3, entity ZN (non-polymer), 65.409 × 2 Da

Total weight

8705.324 Da

Max. entity weight

7270.051 Da

Entity Connection

na 8 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS13:SG	3:ZN1:ZN
2	na	sing	1:CYS16:SG	3:ZN1:ZN
3	na	sing	1:CYS40:SG	3:ZN1:ZN
4	na	sing	1:CYS43:SG	3:ZN1:ZN
5	na	sing	1:CYS28:SG	3:ZN1:ZN
6	na	sing	1:CYS31:SG	3:ZN1:ZN
7	na	sing	1:HIS53:ND1	3:ZN1:ZN
8	na	sing	1:HIS57:NE2	3:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.7 %, Completeness: 71.8 %, Completeness (bb): 71.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	71.8 % (612 of 852)	81.0 % (362 of 447)	56.3 % (184 of 327)	84.6 % (66 of 78)
Backbone	71.5 % (319 of 446)	87.6 % (134 of 153)	56.3 % (125 of 222)	84.5 % (60 of 71)
Sidechain	74.2 % (353 of 476)	77.9 % (229 of 294)	67.4 % (118 of 175)	85.7 % (6 of 7)
Aromatic	38.3 % (23 of 60)	70.0 % (21 of 30)	3.4 % (1 of 29)	100.0 % (1 of 1)
Methyl	85.7 % (48 of 56)	89.3 % (25 of 28)	82.1 % (23 of 28)

1. entity 1

GPLGSVQHVG FKCDNCGIEP IQGVRWHCQD CPPEMSLDFC DSCSDCLHET DIHKEDHQLE PIYR

2. entity 2

ARTKQTARKS TG

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 2 mM [U-13C; U-15N] ZZ domain of ZZZ3, 10 mM Histone H3, 100 mM sodium chloride, 20 mM TRIS, 7 % [U-99% 2H] D2O, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	ZZ domain of ZZZ3	[U-13C; U-15N]	2 mM
2	Histone H3	natural abundance	10 mM
3	sodium chloride	natural abundance	100 mM
4	TRIS	natural abundance	20 mM
5	D2O	[U-99% 2H]	7 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 4 mM [U-13C; U-15N] ZZ domain of ZZZ3, 100 mM sodium chloride, 20 mM TRIS, 7 % [U-99% 2H] D2O, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
6	ZZ domain of ZZZ3	[U-13C; U-15N]	4 mM
7	sodium chloride	natural abundance	100 mM
8	TRIS	natural abundance	20 mM
9	D2O	[U-99% 2H]	7 %

Release date

2018-08-28

Citation

The ZZ-type zinc finger of ZZZ3 modulates the ATAC complex-mediated histone acetylation and gene activation
Mi, W., Zhang, Y., Lyu, J., Wang, X., Tong, Q., Peng, D., Xue, Y., Tencer, A.H., Wen, H., Li, W., Kutateladze, T.G., Shi, X.
Nat. Commun. (2018), 9, 3759-3759, PubMed 30217978 , DOI 10.1038/s41467-018-06247-5 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 30502 released on 2018-08-07
Title Solution structure of ZZZ3 ZZ domain in complex with histone H3K4ac peptide

Related entities 1. ZZ-type zinc finger-containing protein 3, Histone H3, entity 1, : 1 : 1 : 3 : 57 entities Detail

Related entities 2. ZZ-type zinc finger-containing protein 3, Histone H3, entity 2, : 106 : 1 entities Detail

Interaction partners 1. ZZ-type zinc finger-containing protein 3, Histone H3, entity 1, : 16 interactors Detail

More details for 16 interactors identified by 13 citations

Interaction partners 2. ZZ-type zinc finger-containing protein 3, Histone H3, entity 2, : 2 interactors Detail

More details for 2 interactors identified by 2 citations

Experiments performed 13 experiments Detail

1 3D CBCA(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 2 mM [U-13C; U-15N] ZZ domain of ZZZ3, 10 mM Histone H3, 100 mM sodium chloride, 20 mM TRIS, 7 % [U-99% 2H] D2O, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	ZZ domain of ZZZ3	[U-13C; U-15N]	2 mM
2	Histone H3	natural abundance	10 mM
3	sodium chloride	natural abundance	100 mM
4	TRIS	natural abundance	20 mM
5	D2O	[U-99% 2H]	7 %

2 3D 1H-13C NOESY aliphatic

Instrument

Varian INOVA - 900 MHz

Sample

#	Name	Isotope labeling	Concentration
1	ZZ domain of ZZZ3	[U-13C; U-15N]	2 mM
2	Histone H3	natural abundance	10 mM
3	sodium chloride	natural abundance	100 mM
4	TRIS	natural abundance	20 mM
5	D2O	[U-99% 2H]	7 %

3 3D filtered 1H-13C NOESY aliphatic

Instrument

Varian INOVA - 900 MHz

Sample

#	Name	Isotope labeling	Concentration
1	ZZ domain of ZZZ3	[U-13C; U-15N]	2 mM
2	Histone H3	natural abundance	10 mM
3	sodium chloride	natural abundance	100 mM
4	TRIS	natural abundance	20 mM
5	D2O	[U-99% 2H]	7 %

4 3D filtered 1H-15N NOESY

Instrument

Varian INOVA - 900 MHz

Sample

#	Name	Isotope labeling	Concentration
1	ZZ domain of ZZZ3	[U-13C; U-15N]	2 mM
2	Histone H3	natural abundance	10 mM
3	sodium chloride	natural abundance	100 mM
4	TRIS	natural abundance	20 mM
5	D2O	[U-99% 2H]	7 %

5 3D HBHA(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	ZZ domain of ZZZ3	[U-13C; U-15N]	2 mM
2	Histone H3	natural abundance	10 mM
3	sodium chloride	natural abundance	100 mM
4	TRIS	natural abundance	20 mM
5	D2O	[U-99% 2H]	7 %

6 3D 1H-15N NOESY

Instrument

Varian INOVA - 900 MHz

Sample

#	Name	Isotope labeling	Concentration
1	ZZ domain of ZZZ3	[U-13C; U-15N]	2 mM
2	Histone H3	natural abundance	10 mM
3	sodium chloride	natural abundance	100 mM
4	TRIS	natural abundance	20 mM
5	D2O	[U-99% 2H]	7 %

7 3D HCCH-TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	ZZ domain of ZZZ3	[U-13C; U-15N]	2 mM
2	Histone H3	natural abundance	10 mM
3	sodium chloride	natural abundance	100 mM
4	TRIS	natural abundance	20 mM
5	D2O	[U-99% 2H]	7 %

8 2D 1H-1H TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	ZZ domain of ZZZ3	[U-13C; U-15N]	2 mM
2	Histone H3	natural abundance	10 mM
3	sodium chloride	natural abundance	100 mM
4	TRIS	natural abundance	20 mM
5	D2O	[U-99% 2H]	7 %

9 3D HNCA

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 4 mM [U-13C; U-15N] ZZ domain of ZZZ3, 100 mM sodium chloride, 20 mM TRIS, 7 % [U-99% 2H] D2O, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
6	ZZ domain of ZZZ3	[U-13C; U-15N]	4 mM
7	sodium chloride	natural abundance	100 mM
8	TRIS	natural abundance	20 mM
9	D2O	[U-99% 2H]	7 %

10 3D CBCA(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
6	ZZ domain of ZZZ3	[U-13C; U-15N]	4 mM
7	sodium chloride	natural abundance	100 mM
8	TRIS	natural abundance	20 mM
9	D2O	[U-99% 2H]	7 %

11 3D HBHA(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
6	ZZ domain of ZZZ3	[U-13C; U-15N]	4 mM
7	sodium chloride	natural abundance	100 mM
8	TRIS	natural abundance	20 mM
9	D2O	[U-99% 2H]	7 %

12 3D HCCH-TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
6	ZZ domain of ZZZ3	[U-13C; U-15N]	4 mM
7	sodium chloride	natural abundance	100 mM
8	TRIS	natural abundance	20 mM
9	D2O	[U-99% 2H]	7 %

13 3D HNCACB

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
6	ZZ domain of ZZZ3	[U-13C; U-15N]	4 mM
7	sodium chloride	natural abundance	100 mM
8	TRIS	natural abundance	20 mM
9	D2O	[U-99% 2H]	7 %

nullKeywords ZZ domain, ZZZ3, chromatin, gene regulation, histone

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Found 1 Document (1.173 seconds)

BMRB: 30501

Sample #1

Sample #2

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. ZZ-type zinc finger-containing protein 3, Histone H3, entity 1, : 1 : 1 : 3 : 57 entities Detail

Related entities 2. ZZ-type zinc finger-containing protein 3, Histone H3, entity 2, : 106 : 1 entities Detail

Interaction partners 1. ZZ-type zinc finger-containing protein 3, Histone H3, entity 1, : 16 interactors Detail

Interaction partners 2. ZZ-type zinc finger-containing protein 3, Histone H3, entity 2, : 2 interactors Detail

Experiments performed 13 experiments Detail

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