BMRB: 30547

RNA Duplex containing the internal loop 5'-GCAU/3'-UACG

Authors

Berger, K.D., Kennedy, S.D., Turner, D.H.

Assembly

RNA (5'-R(*CP*GP*GP*GP*CP*AP*UP*CP*CP*G)-3')

Entity

1. RNA (5'-R(*CP*GP*GP*GP*CP*AP*UP*CP*CP*G)-3') (polymer, Thiol state: not present), 10 monomers, 3254.936 × 2 Da Detail

CGGGCAUCCG

Total weight

6509.872 Da

Max. entity weight

3254.936 Da

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 67.3 %, Completeness (bb): 61.7 % Detail

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Polymer type: polyribonucleotide

	Total	1H	13C	31P
All	67.3 % (111 of 165)	98.9 % (88 of 89)	27.3 % (18 of 66)	50.0 % (5 of 10)
Suger, PO4	61.7 % (74 of 120)	100.0 % (60 of 60)	18.0 % (9 of 50)	50.0 % (5 of 10)
Nucleobase	82.2 % (37 of 45)	96.6 % (28 of 29)	56.3 % (9 of 16)
Aromatic	78.4 % (29 of 37)	95.2 % (20 of 21)	56.3 % (9 of 16)

1. RNA (5'-R(*CP*GP*GP*GP*CP*AP*UP*CP*CP*G)-3')

CGGGCAUCCG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 288 K, pH 6.2, Details 1 mM RNA (5'-R(*CP*GP*GP*GP*CP*AP*UP*CP*CP*G)-3'), 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	RNA (5'-R(*CP*GP*GP*GP*CP*AP*UP*CP*CP*G)-3')	none		1 mM
2	sodium chloride	natural abundance		80 mM
3	sodium phosphate	natural abundance		50 mM

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Release date

2018-12-12

Citation

Nuclear Magnetic Resonance Reveals That GU Base Pairs Flanking Internal Loops Can Adopt Diverse Structures
Berger, K.D., Kennedy, S.D., Turner, D.H.
Biochemistry (2019), 58, 1094-1108, PubMed 30702283 , DOI 10.1021/acs.biochem.8b01027 , Abstract

Entries sharing articles BMRB: 2 entries Detail

Experiments performed 3 experiments Detail