Solution structure of a c-JUN 5' UTR stem-loop associated with specialized cap-dependent translation initiation (WT apical loop)
Polymer type: polyribonucleotide
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 75.6 % (691 of 914) | 77.4 % (388 of 501) | 75.6 % (291 of 385) | 42.9 % (12 of 28) |
Suger, PO4 | 77.8 % (479 of 616) | 78.9 % (265 of 336) | 76.4 % (214 of 280) | |
Nucleobase | 71.1 % (212 of 298) | 74.5 % (123 of 165) | 73.3 % (77 of 105) | 42.9 % (12 of 28) |
Aromatic | 74.1 % (197 of 266) | 81.2 % (108 of 133) | 73.3 % (77 of 105) | 42.9 % (12 of 28) |
1. entity 1
GGCAGUAUAG UCCGAACUGC AAAUCUUAUU UUCUUUUCAC CUUCUCUCUA ACUGCCSolvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.1, Details 1.0 mM [A,G-98% D3', 4', 5', 5"; C,U-98% D3', 4', 5', 5", 5] RNA, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | RNA | [A,G-98% D3', 4', 5', 5"; C,U-98% D3', 4', 5', 5", 5] | 1.0 mM | |
2 | Sodium Phosphate | natural abundance | 20 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.1, Details 1.0 mM RNA, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
3 | RNA | natural abundance | 1.0 mM | |
4 | Sodium Phosphate | natural abundance | 20 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.1, Details 1.0 mM RNA, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | RNA | natural abundance | 1.0 mM | |
6 | Sodium Phosphate | natural abundance | 20 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.1, Details 1.0 mM RNA, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | RNA | natural abundance | 1.0 mM | |
8 | Sodium Phosphate | natural abundance | 20 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.1, Details 1.0 mM RNA, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
9 | RNA | natural abundance | 1.0 mM | |
10 | Sodium Phosphate | natural abundance | 20 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.1, Details 1.0 mM [A,G-98% D3', 4', 5', 5"; C,U-98% D3', 4', 5', 5", 5] RNA, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
11 | RNA | [A,G-98% D3', 4', 5', 5"; C,U-98% D3', 4', 5', 5", 5] | 1.0 mM | |
12 | Sodium Phosphate | natural abundance | 20 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.1, Details 1.0 mM [A,C,G,U-98% 13C, A,C,G,U-98% 15N] RNA, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
13 | RNA | [A,C,G,U-98% 13C, A,C,G,U-98% 15N] | 1.0 mM | |
14 | Sodium Phosphate | natural abundance | 20 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.1, Details 1.0 mM [A,C,G,U-98% 13C, A,C,G,U-98% 15N] RNA, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
15 | RNA | [A,C,G,U-98% 13C, A,C,G,U-98% 15N] | 1.0 mM | |
16 | Sodium Phosphate | natural abundance | 20 mM |