NMR solution structure of YfiD
MITGIQITKA ANDDLLNSFW LLDSEKGEAR CIVAKAGFAE DEVVAVSKLG DIEYREVPVE VKPEVRVEGG QHLNVNVLRR ETLEDAVKHP EKYPQLTIRV SGYAVRFNSL TPEQQRDVIA RTFTESL
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 31.8 % (469 of 1474) | 40.3 % (308 of 765) | 17.5 % (101 of 576) | 45.1 % (60 of 133) |
Backbone | 36.4 % (274 of 752) | 47.7 % (122 of 256) | 24.6 % (92 of 374) | 49.2 % (60 of 122) |
Sidechain | 25.7 % (216 of 842) | 36.0 % (183 of 509) | 10.2 % (33 of 322) | 0.0 % (0 of 11) |
Aromatic | 0.0 % (0 of 84) | 0.0 % (0 of 42) | 0.0 % (0 of 41) | 0.0 % (0 of 1) |
Methyl | 21.1 % (35 of 166) | 34.9 % (29 of 83) | 7.2 % (6 of 83) |
1. entity 1
MITGIQITKA ANDDLLNSFW LLDSEKGEAR CIVAKAGFAE DEVVAVSKLG DIEYREVPVE VKPEVRVEGG QHLNVNVLRR ETLEDAVKHP EKYPQLTIRV SGYAVRFNSL TPEQQRDVIA RTFTESLSolvent system 90% H2O/10% D2O, Pressure 760 mmHg, Temperature 293 K, pH 7.2, Details 0.7 mM [U-15N] YfiD, 18 mM HEPES, 2.7 mM ammonium sulfate, 10 % v/v D2O, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | YfiD | [U-15N] | 0.7 mM | |
2 | HEPES | natural abundance | 18 mM | |
3 | ammonium sulfate | natural abundance | 2.7 mM | |
4 | D2O | natural abundance | 10 % v/v |
Solvent system 90% H2O/10% D2O, Pressure 760 mmHg, Temperature 293 K, pH 7.2, Details 1.3 mM [U-100% 13C; U-100% 15N] YfiD, 18 mM HEPES, 2.7 mM ammonium sulfate, 10 % v/v D2O, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | YfiD | [U-100% 13C; U-100% 15N] | 1.3 mM | |
6 | HEPES | natural abundance | 18 mM | |
7 | ammonium sulfate | natural abundance | 2.7 mM | |
8 | D2O | natural abundance | 10 % v/v |