BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	46.4 % (497 of 1071)	37.6 % (209 of 556)	47.9 % (201 of 420)	91.6 % (87 of 95)
Backbone	84.5 % (453 of 536)	99.4 % (179 of 180)	69.8 % (187 of 268)	98.9 % (87 of 88)
Sidechain	21.2 % (132 of 623)	8.0 % (30 of 376)	42.5 % (102 of 240)	0.0 % (0 of 7)
Aromatic	5.4 % (4 of 74)	5.4 % (2 of 37)	5.6 % (2 of 36)	0.0 % (0 of 1)
Methyl	8.3 % (9 of 108)	5.6 % (3 of 54)	11.1 % (6 of 54)

1. entity 1

MAEYGTLLQD LTNNITLEDL EQLKSACKED IPSEKSEEIT TGSAWFSFLE SHNKLDKDNL SYIEHIFEIS RRPDLLTMVV DYRTRVLKIS

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0 (±0.01), Details 10 mM sodium phosphate, 0.1 mM dithiothreitol, 50 uM sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	entity_1		0.0 ~ 0.0 mM
2	sodium phosphate	natural abundance	10 mM
3	dithiothreitol	natural abundance	0.1 mM
4	sodium azide	natural abundance	50 uM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	liquid anhydrous ammonia	nitrogen	internal	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	liquid anhydrous ammonia	nitrogen	internal	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	liquid anhydrous ammonia	nitrogen	internal	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 6P6B, Strand ID: A Detail

Release date

2019-07-14

Citation

PEA-15 C-Terminal Tail Allosterically Modulates Death-Effector Domain Conformation and Facilitates Protein-Protein Interactions
Crespo Flores, S., Cabezas, A., Hassan, S., Wei, Y.
Int. J. Mol. Sci. (2019), 20, 3335-3335, PubMed 31284641 , DOI 10.3390/ijms20133335 , Abstract

Related entities 1. Astrocytic phosphoprotein PEA-15, : 1 : 11 : 28 entities Detail

Interaction partners 1. Astrocytic phosphoprotein PEA-15, : 13 interactors Detail

More details for 13 interactors identified by 6 citations

Experiments performed 3 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr30613_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr30613_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	OK	444		100.0 (chain: 1, length: 90)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 90, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 179
  - ¹³C chemical shifts: 178
  - ¹⁵N chemical shifts: 87
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	556	179	32.2
¹³C chemical shifts	420	178	42.4
¹⁵N chemical shifts	99	87	87.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	180	179	99.4
¹³C chemical shifts	180	90	50.0
¹⁵N chemical shifts	88	87	98.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	376	0	0.0
¹³C chemical shifts	240	88	36.7
¹⁵N chemical shifts	11	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	56	0	0.0
¹³C chemical shifts	56	3	5.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	37	0	0.0
¹³C chemical shifts	36	0	0.0
¹⁵N chemical shifts	1	0	0.0

Keywords APOPTOSIS, MAP kinase, protein-protein interaction

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Found 1 Document (0.739 seconds)

BMRB: 30613

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Astrocytic phosphoprotein PEA-15, : 1 : 11 : 28 entities Detail

Interaction partners 1. Astrocytic phosphoprotein PEA-15, : 13 interactors Detail

Experiments performed 3 experiments Detail

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Sample

Chemical shift validation 3 contents Detail