BMRBj - BMREntryMaster

Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-I2I5G8 PISCIDIN-3, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
1	PISCIDIN-3	[15N]-I2I5G8	10 (±1.0) mM
2	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
3	Cholesterol	natural abundance	80 (±15.0) mM

Sample #2

Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-V10G13I16 PISCIDIN-3, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
4	PISCIDIN-3	[15N]-V10G13I16	10 (±1.0) mM
5	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
6	Cholesterol	natural abundance	80 (±15.0) mM

Sample #3

Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-G17F19L20 PISCIDIN-3, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
7	PISCIDIN-3	[15N]-G17F19L20	10 (±1.0) mM
8	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
9	Cholesterol	natural abundance	80 (±15.0) mM

Sample #4

Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-F1S15 PISCIDIN-3, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
10	PISCIDIN-3	[15N]-F1S15	10 (±1.0) mM
11	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
12	Cholesterol	natural abundance	80 (±15.0) mM

Sample #5

Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-R14 PISCIDIN-3, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
13	PISCIDIN-3	[15N]-R14	10 (±1.0) mM
14	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
15	Cholesterol	natural abundance	80 (±15.0) mM

Sample #6

Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-H4 PISCIDIN-3, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
16	PISCIDIN-3	[15N]-H4	10 (±1.0) mM
17	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
18	Cholesterol	natural abundance	80 (±15.0) mM

Sample #7

Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-R18 PISCIDIN-3, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
19	PISCIDIN-3	[15N]-R18	10 (±1.0) mM
20	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
21	Cholesterol	natural abundance	80 (±15.0) mM

Sample #8

Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-F6I9A12 PISCIDIN-3, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
22	PISCIDIN-3	[15N]-F6I9A12	10 (±1.0) mM
23	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
24	Cholesterol	natural abundance	80 (±15.0) mM

Sample #9

Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-F6I9 PISCIDIN-3, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
25	PISCIDIN-3	[15N]-F6I9	10 (±1.0) mM
26	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
27	Cholesterol	natural abundance	80 (±15.0) mM

Sample #10

Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-R7 PISCIDIN-3, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
28	PISCIDIN-3	[15N]-R7	10 (±1.0) mM
29	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
30	Cholesterol	natural abundance	80 (±15.0) mM

Sample #11

Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-T21G22 PISCIDIN-3, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
31	PISCIDIN-3	[15N]-T21G22	10 (±1.0) mM
32	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
33	Cholesterol	natural abundance	80 (±15.0) mM

Sample #12

Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-H11 PISCIDIN-3, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
34	PISCIDIN-3	[15N]-H11	10 (±1.0) mM
35	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
36	Cholesterol	natural abundance	80 (±15.0) mM

Sample #13

Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-H3 PISCIDIN-3, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
37	PISCIDIN-3	[15N]-H3	10 (±1.0) mM
38	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
39	Cholesterol	natural abundance	80 (±15.0) mM

Sample #14

Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-A12G17 PISCIDIN-3, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
40	PISCIDIN-3	[15N]-A12G17	10 (±1.0) mM
41	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
42	Cholesterol	natural abundance	80 (±15.0) mM

Protein Blocks Logo

Calculated from 10 models in PDB: 6PEZ, Strand ID: A Detail

Release date

2019-10-08

Citation

The host-defense peptide piscidin P1 reorganizes lipid domains in membranes and decreases activation energies in mechanosensitive ion channels
Comert, F., Heinrich, F., Chowdhury, A., Schoeneck, M., Darling, C., Anderson, K.W., Libardo, M., Angeles-Boza, A.M., Silin, V., Cotten, M.L., Mihailescu, M.
J. Biol. Chem. (2019), 11, 12620-12620, PubMed 31619519 , DOI 10.1074/jbc.RA119.010232 , Abstract

Related entities 1. Piscidin-3, : 1 : 3 : 11 entities Detail

Experiments performed 14 experiments Detail

1 15N_1H solid-state de-HETCOR

Instrument

Bruker AVANCE - 600 MHz National High Magnetic Field Laboratory

Sample

State anisotropic, Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-I2I5G8 PISCIDIN-3, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
1	PISCIDIN-3	[15N]-I2I5G8	10 (±1.0) mM
2	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
3	Cholesterol	natural abundance	80 (±15.0) mM

2 15N_1H solid-state de-HETCOR

Instrument

Bruker AVANCE - 900 MHz National High Magnetic Field Laboratory

Sample

State anisotropic, Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-V10G13I16 PISCIDIN-3, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
4	PISCIDIN-3	[15N]-V10G13I16	10 (±1.0) mM
5	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
6	Cholesterol	natural abundance	80 (±15.0) mM

3 15N_1H solid-state de-HETCOR

Instrument

Bruker AVANCE - 900 MHz National High Magnetic Field Laboratory

Sample

State anisotropic, Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-G17F19L20 PISCIDIN-3, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
7	PISCIDIN-3	[15N]-G17F19L20	10 (±1.0) mM
8	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
9	Cholesterol	natural abundance	80 (±15.0) mM

4 15N_1H solid-state de-HETCOR

Instrument

Bruker AVANCE - 600 MHz Rennselaer Polytechnic Institute

Sample

State anisotropic, Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-F1S15 PISCIDIN-3, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
10	PISCIDIN-3	[15N]-F1S15	10 (±1.0) mM
11	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
12	Cholesterol	natural abundance	80 (±15.0) mM

5 15N_1H solid-state de-HETCOR

Instrument

Bruker AVANCE - 600 MHz Rennselaer Polytechnic Institute

Sample

State anisotropic, Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-F6I9 PISCIDIN-3, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
25	PISCIDIN-3	[15N]-F6I9	10 (±1.0) mM
26	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
27	Cholesterol	natural abundance	80 (±15.0) mM

6 15N_1H solid-state de-HETCOR

Instrument

Bruker AVANCE - 600 MHz National High Magnetic Field Laboratory

Sample

State anisotropic, Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-A12G17 PISCIDIN-3, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
40	PISCIDIN-3	[15N]-A12G17	10 (±1.0) mM
41	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
42	Cholesterol	natural abundance	80 (±15.0) mM

7 15N_1H solid-state de-HETCOR

Instrument

Bruker AVANCE - 600 MHz National High Magnetic Field Laboratory

Sample

State anisotropic, Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-H3 PISCIDIN-3, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
37	PISCIDIN-3	[15N]-H3	10 (±1.0) mM
38	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
39	Cholesterol	natural abundance	80 (±15.0) mM

8 15N_1H solid-state de-HETCOR

Instrument

Bruker AVANCE - 600 MHz National High Magnetic Field Laboratory

Sample

State anisotropic, Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-H11 PISCIDIN-3, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
34	PISCIDIN-3	[15N]-H11	10 (±1.0) mM
35	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
36	Cholesterol	natural abundance	80 (±15.0) mM

9 15N_1H solid-state de-HETCOR

Instrument

Bruker AVANCE - 900 MHz National High Magnetic Field Laboratory

Sample

State anisotropic, Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-T21G22 PISCIDIN-3, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
31	PISCIDIN-3	[15N]-T21G22	10 (±1.0) mM
32	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
33	Cholesterol	natural abundance	80 (±15.0) mM

10 15N_1H solid-state de-HETCOR

Instrument

Bruker AVANCE - 900 MHz National High Magnetic Field Laboratory

Sample

State anisotropic, Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-R7 PISCIDIN-3, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
28	PISCIDIN-3	[15N]-R7	10 (±1.0) mM
29	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
30	Cholesterol	natural abundance	80 (±15.0) mM

11 15N_1H solid-state de-HETCOR

Instrument

Bruker AVANCE - 900 MHz National High Magnetic Field Laboratory

Sample

State anisotropic, Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-R14 PISCIDIN-3, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
13	PISCIDIN-3	[15N]-R14	10 (±1.0) mM
14	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
15	Cholesterol	natural abundance	80 (±15.0) mM

12 15N_1H solid-state de-HETCOR

Instrument

Bruker AVANCE - 600 MHz National High Magnetic Field Laboratory

Sample

State anisotropic, Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-H4 PISCIDIN-3, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
16	PISCIDIN-3	[15N]-H4	10 (±1.0) mM
17	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
18	Cholesterol	natural abundance	80 (±15.0) mM

13 15N_1H solid-state de-HETCOR

Instrument

Bruker AVANCE - 900 MHz National High Magnetic Field Laboratory

Sample

State anisotropic, Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-R18 PISCIDIN-3, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
19	PISCIDIN-3	[15N]-R18	10 (±1.0) mM
20	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
21	Cholesterol	natural abundance	80 (±15.0) mM

14 15N_1H solid-state de-HETCOR

Instrument

Bruker AVANCE - 600 MHz Rennselaer Polytechnic Institute

Sample

State anisotropic, Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-F6I9A12 PISCIDIN-3, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
22	PISCIDIN-3	[15N]-F6I9A12	10 (±1.0) mM
23	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
24	Cholesterol	natural abundance	80 (±15.0) mM

Chemical shift validation 3 contents Detail

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	20		87.0 (chain: 1, length: 23)
1	Chemical shift references	chem_shift_reference_1	OK	2		No information

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹⁵N chemical shifts	26	20	76.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹⁵N chemical shifts	23	20	87.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹⁵N chemical shifts	3	0	0.0

Keywords AMPHIPATHIC, ANTI HIV-1, ANTICANCER PEPTIDE, ANTIMICROBIAL PEPTIDE, ANTIMICROBIAL PROTEIN, BACTERIAL CELL MEMBRANE MIMIC, CATIONIC, HELICAL, HISTIDINE RICH, LIPID BILAYERS

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr30615_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

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BMRB: 30615

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Related entities 1. Piscidin-3, : 1 : 3 : 11 entities Detail

Experiments performed 14 experiments Detail

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Chemical shift validation 3 contents Detail