BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	82.5 % (781 of 947)	80.5 % (396 of 492)	85.9 % (317 of 369)	79.1 % (68 of 86)
Backbone	79.8 % (415 of 520)	76.7 % (145 of 189)	82.7 % (206 of 249)	78.0 % (64 of 82)
Sidechain	86.7 % (432 of 498)	82.8 % (251 of 303)	92.7 % (177 of 191)	100.0 % (4 of 4)
Aromatic	94.7 % (36 of 38)	94.7 % (18 of 19)	94.4 % (17 of 18)	100.0 % (1 of 1)
Methyl	91.8 % (90 of 98)	91.8 % (45 of 49)	91.8 % (45 of 49)

1. entity 1

METATAGEWQ GKGSGGSGGS GGSQDLYATL DVPAPIAVVG GKVRAMTLEG PVEVAVPPRT QAGRKLRLKG KGFPGPAGRG DLYLEVRIT

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 1 mM [U-13C; U-15N] PhoA_235-245_DnaJ_191-256, 20 mM potassium phosphate, 75 mM potassium chloride, 0.04 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	PhoA_235-245_DnaJ_191-256	[U-13C; U-15N]	1 mM
2	potassium phosphate	natural abundance	20 mM
3	potassium chloride	natural abundance	75 mM
4	sodium azide	natural abundance	0.04 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.55 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 6PQE, Strand ID: A Detail

Release date

2019-09-05

Citation

Structural basis for client recognition and activity of Hsp40 chaperones
Jiang, Y., Rossi, P., Kalodimos, C.G.
Science (2019), 365, 1313-1319, PubMed 31604242 , DOI 10.1126/science.aax1280 , Abstract

Related entities 1. Alkaline phosphatase,Chaperone protein DnaJ 2 (E.C.3.1.3.1), : 1 : 1 : 11 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-13C HSQC

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 1 mM [U-13C; U-15N] PhoA_235-245_DnaJ_191-256, 20 mM potassium phosphate, 75 mM potassium chloride, 0.04 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	PhoA_235-245_DnaJ_191-256	[U-13C; U-15N]	1 mM
2	potassium phosphate	natural abundance	20 mM
3	potassium chloride	natural abundance	75 mM
4	sodium azide	natural abundance	0.04 %

2 3D HNCO

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	PhoA_235-245_DnaJ_191-256	[U-13C; U-15N]	1 mM
2	potassium phosphate	natural abundance	20 mM
3	potassium chloride	natural abundance	75 mM
4	sodium azide	natural abundance	0.04 %

3 3D HBHA(CO)NH

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	PhoA_235-245_DnaJ_191-256	[U-13C; U-15N]	1 mM
2	potassium phosphate	natural abundance	20 mM
3	potassium chloride	natural abundance	75 mM
4	sodium azide	natural abundance	0.04 %

4 3D CCH-NOESY

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	PhoA_235-245_DnaJ_191-256	[U-13C; U-15N]	1 mM
2	potassium phosphate	natural abundance	20 mM
3	potassium chloride	natural abundance	75 mM
4	sodium azide	natural abundance	0.04 %

5 3D CCH_TOCSY

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	PhoA_235-245_DnaJ_191-256	[U-13C; U-15N]	1 mM
2	potassium phosphate	natural abundance	20 mM
3	potassium chloride	natural abundance	75 mM
4	sodium azide	natural abundance	0.04 %

6 3D H(CCO)NH

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	PhoA_235-245_DnaJ_191-256	[U-13C; U-15N]	1 mM
2	potassium phosphate	natural abundance	20 mM
3	potassium chloride	natural abundance	75 mM
4	sodium azide	natural abundance	0.04 %

7 3D HNCACB

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	PhoA_235-245_DnaJ_191-256	[U-13C; U-15N]	1 mM
2	potassium phosphate	natural abundance	20 mM
3	potassium chloride	natural abundance	75 mM
4	sodium azide	natural abundance	0.04 %

8 3D 1H-15N NOESY

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	PhoA_235-245_DnaJ_191-256	[U-13C; U-15N]	1 mM
2	potassium phosphate	natural abundance	20 mM
3	potassium chloride	natural abundance	75 mM
4	sodium azide	natural abundance	0.04 %

9 3D 1H-13C NOESY

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	PhoA_235-245_DnaJ_191-256	[U-13C; U-15N]	1 mM
2	potassium phosphate	natural abundance	20 mM
3	potassium chloride	natural abundance	75 mM
4	sodium azide	natural abundance	0.04 %

10 2D 1H-15N HSQC

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	PhoA_235-245_DnaJ_191-256	[U-13C; U-15N]	1 mM
2	potassium phosphate	natural abundance	20 mM
3	potassium chloride	natural abundance	75 mM
4	sodium azide	natural abundance	0.04 %

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr30629_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr30629_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	850		87.6 (chain: 1, length: 89)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 89, Sequence coverage: 87.6%
- Total number of assignments
  - ¹H chemical shifts: 477
  - ¹³C chemical shifts: 308
  - ¹⁵N chemical shifts: 65
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	492	385	78.3
¹³C chemical shifts	369	308	83.5
¹⁵N chemical shifts	92	65	70.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	189	140	74.1
¹³C chemical shifts	178	133	74.7
¹⁵N chemical shifts	82	61	74.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	303	245	80.9
¹³C chemical shifts	191	175	91.6
¹⁵N chemical shifts	10	4	40.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	51	46	90.2
¹³C chemical shifts	51	46	90.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	19	18	94.7
¹³C chemical shifts	18	17	94.4
¹⁵N chemical shifts	1	1	100.0

Keywords CHAPERONE, Client Recognition

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Link destinations for BMRB, PDB and EMDB

Found 1 Document (0.693 seconds)

BMRB: 30629

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Alkaline phosphatase,Chaperone protein DnaJ 2 (E.C.3.1.3.1), : 1 : 1 : 11 entities Detail

Experiments performed 10 experiments Detail

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Chemical shift validation 3 contents Detail