BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	80.2 % (766 of 955)	77.1 % (383 of 497)	85.6 % (320 of 374)	75.0 % (63 of 84)
Backbone	80.7 % (418 of 518)	79.1 % (148 of 187)	82.8 % (207 of 250)	77.8 % (63 of 81)
Sidechain	81.3 % (414 of 509)	75.8 % (235 of 310)	91.3 % (179 of 196)	0.0 % (0 of 3)
Aromatic	52.6 % (20 of 38)	52.6 % (10 of 19)	52.6 % (10 of 19)
Methyl	99.0 % (101 of 102)	98.0 % (50 of 51)	100.0 % (51 of 51)

1. entity 1

MSQLRIAAYG PHGSGGSGGS GGSQDLYATL DVPAPIAVVG GKVRAMTLEG PVEVAVPPRT QAGRKLRLKG KGFPGPAGRG DLYLEVRIT

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 1 mM [U-100% 13C; U-100% 15N] F-CBD2, 75 mM potassium chloride, 20 mM potassium phosphate, 0.04 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	F-CBD2	[U-100% 13C; U-100% 15N]	1 mM
2	potassium chloride	natural abundance	75 mM
3	potassium phosphate	natural abundance	20 mM
4	sodium azide	natural abundance	0.04 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.61 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 6PRI, Strand ID: A Detail

Release date

2019-08-06

Citation

Structural basis for client recognition and activity of Hsp40 chaperones
Jiang, Y., Rossi, P., Kalodimos, C.G.
Science (2019), 365, 1313-1319, PubMed 31604242 , DOI 10.1126/science.aax1280 , Abstract

Related entities 1. Alkaline phosphatase,Chaperone protein DnaJ 2 (E.C.3.1.3.1), : 1 : 3 : 16 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 1 mM [U-100% 13C; U-100% 15N] F-CBD2, 75 mM potassium chloride, 20 mM potassium phosphate, 0.04 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	F-CBD2	[U-100% 13C; U-100% 15N]	1 mM
2	potassium chloride	natural abundance	75 mM
3	potassium phosphate	natural abundance	20 mM
4	sodium azide	natural abundance	0.04 %

2 2D 1H-13C HSQC

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	F-CBD2	[U-100% 13C; U-100% 15N]	1 mM
2	potassium chloride	natural abundance	75 mM
3	potassium phosphate	natural abundance	20 mM
4	sodium azide	natural abundance	0.04 %

3 3D HNCACB

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	F-CBD2	[U-100% 13C; U-100% 15N]	1 mM
2	potassium chloride	natural abundance	75 mM
3	potassium phosphate	natural abundance	20 mM
4	sodium azide	natural abundance	0.04 %

4 3D HNCO

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	F-CBD2	[U-100% 13C; U-100% 15N]	1 mM
2	potassium chloride	natural abundance	75 mM
3	potassium phosphate	natural abundance	20 mM
4	sodium azide	natural abundance	0.04 %

5 3D HBHA(CO)NH

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	F-CBD2	[U-100% 13C; U-100% 15N]	1 mM
2	potassium chloride	natural abundance	75 mM
3	potassium phosphate	natural abundance	20 mM
4	sodium azide	natural abundance	0.04 %

6 3D CCONH tocsy

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	F-CBD2	[U-100% 13C; U-100% 15N]	1 mM
2	potassium chloride	natural abundance	75 mM
3	potassium phosphate	natural abundance	20 mM
4	sodium azide	natural abundance	0.04 %

7 3D CCH-NOESY

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	F-CBD2	[U-100% 13C; U-100% 15N]	1 mM
2	potassium chloride	natural abundance	75 mM
3	potassium phosphate	natural abundance	20 mM
4	sodium azide	natural abundance	0.04 %

8 3D CCH-TOCSY

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	F-CBD2	[U-100% 13C; U-100% 15N]	1 mM
2	potassium chloride	natural abundance	75 mM
3	potassium phosphate	natural abundance	20 mM
4	sodium azide	natural abundance	0.04 %

9 3D 1H-13C NOESY

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	F-CBD2	[U-100% 13C; U-100% 15N]	1 mM
2	potassium chloride	natural abundance	75 mM
3	potassium phosphate	natural abundance	20 mM
4	sodium azide	natural abundance	0.04 %

10 3D 1H-15N NOESY

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	F-CBD2	[U-100% 13C; U-100% 15N]	1 mM
2	potassium chloride	natural abundance	75 mM
3	potassium phosphate	natural abundance	20 mM
4	sodium azide	natural abundance	0.04 %

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr30634_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr30634_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	864		93.3 (chain: 1, length: 89)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 89, Sequence coverage: 93.3%
- Total number of assignments
  - ¹H chemical shifts: 481
  - ¹³C chemical shifts: 320
  - ¹⁵N chemical shifts: 63
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	497	381	76.7
¹³C chemical shifts	374	320	85.6
¹⁵N chemical shifts	91	63	69.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	187	148	79.1
¹³C chemical shifts	178	141	79.2
¹⁵N chemical shifts	81	63	77.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	310	233	75.2
¹³C chemical shifts	196	179	91.3
¹⁵N chemical shifts	10	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	53	50	94.3
¹³C chemical shifts	53	53	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	19	10	52.6
¹³C chemical shifts	19	10	52.6

Keywords CHAPERONE, Client Recognition

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Found 1 Document (0.579 seconds)

BMRB: 30634

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Alkaline phosphatase,Chaperone protein DnaJ 2 (E.C.3.1.3.1), : 1 : 3 : 16 entities Detail

Experiments performed 10 experiments Detail

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Chemical shift validation 3 contents Detail