BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	85.4 % (822 of 962)	82.4 % (411 of 499)	88.4 % (334 of 378)	90.6 % (77 of 85)
Backbone	87.7 % (456 of 520)	87.2 % (163 of 187)	86.1 % (216 of 251)	93.9 % (77 of 82)
Sidechain	84.9 % (437 of 515)	79.5 % (248 of 312)	94.5 % (189 of 200)	0.0 % (0 of 3)
Aromatic	95.5 % (42 of 44)	95.5 % (21 of 22)	95.5 % (21 of 22)
Methyl	100.0 % (102 of 102)	100.0 % (51 of 51)	100.0 % (51 of 51)

1. entity 1

MQTDLFYTMK AAGSGGSGGS GGSQDLYATL DVPAPIAVVG GKVRAMTLEG PVEVAVPPRT QAGRKLRLKG KGFPGPAGRG DLYLEVRIT

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 1 mM [U-100% 13C; U-100% 15N] G-CBD2, 75 mM potassium chloride, 20 mM potassium phosphate, 0.04 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	G-CBD2	[U-100% 13C; U-100% 15N]	1 mM
2	potassium chloride	natural abundance	75 mM
3	potassium phosphate	natural abundance	20 mM
4	sodium azide	natural abundance	0.04 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.1 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.4 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 6PRJ, Strand ID: A Detail

Release date

2019-08-06

Citation

Structural basis for client recognition and activity of Hsp40 chaperones
Jiang, Y., Rossi, P., Kalodimos, C.G.
Science (2019), 365, 1313-1319, PubMed 31604242 , DOI 10.1126/science.aax1280 , Abstract

Related entities 1. Alkaline phosphatase,Chaperone protein DnaJ 2 (E.C.3.1.3.1), : 1 : 3 : 27 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 1 mM [U-100% 13C; U-100% 15N] G-CBD2, 75 mM potassium chloride, 20 mM potassium phosphate, 0.04 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	G-CBD2	[U-100% 13C; U-100% 15N]	1 mM
2	potassium chloride	natural abundance	75 mM
3	potassium phosphate	natural abundance	20 mM
4	sodium azide	natural abundance	0.04 %

2 2D 1H-13C HSQC

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	G-CBD2	[U-100% 13C; U-100% 15N]	1 mM
2	potassium chloride	natural abundance	75 mM
3	potassium phosphate	natural abundance	20 mM
4	sodium azide	natural abundance	0.04 %

3 3D HNCACB

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	G-CBD2	[U-100% 13C; U-100% 15N]	1 mM
2	potassium chloride	natural abundance	75 mM
3	potassium phosphate	natural abundance	20 mM
4	sodium azide	natural abundance	0.04 %

4 3D HBHA(CO)NH

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	G-CBD2	[U-100% 13C; U-100% 15N]	1 mM
2	potassium chloride	natural abundance	75 mM
3	potassium phosphate	natural abundance	20 mM
4	sodium azide	natural abundance	0.04 %

5 3D HNCO

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	G-CBD2	[U-100% 13C; U-100% 15N]	1 mM
2	potassium chloride	natural abundance	75 mM
3	potassium phosphate	natural abundance	20 mM
4	sodium azide	natural abundance	0.04 %

6 3D H(CCO)NH

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	G-CBD2	[U-100% 13C; U-100% 15N]	1 mM
2	potassium chloride	natural abundance	75 mM
3	potassium phosphate	natural abundance	20 mM
4	sodium azide	natural abundance	0.04 %

7 3D 1H-15N NOESY

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	G-CBD2	[U-100% 13C; U-100% 15N]	1 mM
2	potassium chloride	natural abundance	75 mM
3	potassium phosphate	natural abundance	20 mM
4	sodium azide	natural abundance	0.04 %

8 3D 1H-13C NOESY

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	G-CBD2	[U-100% 13C; U-100% 15N]	1 mM
2	potassium chloride	natural abundance	75 mM
3	potassium phosphate	natural abundance	20 mM
4	sodium azide	natural abundance	0.04 %

9 3D CCH_NOESY

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	G-CBD2	[U-100% 13C; U-100% 15N]	1 mM
2	potassium chloride	natural abundance	75 mM
3	potassium phosphate	natural abundance	20 mM
4	sodium azide	natural abundance	0.04 %

10 3D CCH-TOCSY

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	G-CBD2	[U-100% 13C; U-100% 15N]	1 mM
2	potassium chloride	natural abundance	75 mM
3	potassium phosphate	natural abundance	20 mM
4	sodium azide	natural abundance	0.04 %

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr30635_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr30635_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	909		98.9 (chain: 1, length: 89)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 89, Sequence coverage: 98.9%
- Total number of assignments
  - ¹H chemical shifts: 506
  - ¹³C chemical shifts: 327
  - ¹⁵N chemical shifts: 76
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	499	398	79.8
¹³C chemical shifts	378	327	86.5
¹⁵N chemical shifts	91	76	83.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	187	158	84.5
¹³C chemical shifts	178	140	78.7
¹⁵N chemical shifts	82	76	92.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	312	240	76.9
¹³C chemical shifts	200	187	93.5
¹⁵N chemical shifts	9	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	54	54	100.0
¹³C chemical shifts	54	54	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	22	21	95.5
¹³C chemical shifts	22	21	95.5

Keywords CHAPERONE, Client Recognition

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Found 1 Document (0.666 seconds)

BMRB: 30635

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Alkaline phosphatase,Chaperone protein DnaJ 2 (E.C.3.1.3.1), : 1 : 3 : 27 entities Detail

Experiments performed 10 experiments Detail

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Chemical shift validation 3 contents Detail