Structural Basis for Client Recognition and Activity of Hsp40 Chaperones
AALVAHVTSG SGGSGGSGGS GRDLRAELPL TLEEAFHGGE RVVEVAGRRV SVRIPPGVRE GSVIRVPGMG GQGNPPGDLL LVVRLLPHPV FRLEGQDLYA TLDVPAPIAV VGGKVRAMTL EGPVEVAVPP RTQAGRKLRL KGKGFPGPAG RGDLYLEVRI T
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 43.1 % (753 of 1747) | 30.0 % (272 of 908) | 50.4 % (347 of 689) | 89.3 % (134 of 150) |
Backbone | 56.9 % (533 of 936) | 44.2 % (148 of 335) | 54.9 % (250 of 455) | 92.5 % (135 of 146) |
Sidechain | 34.9 % (329 of 944) | 21.6 % (124 of 573) | 55.9 % (205 of 367) | 0.0 % (0 of 4) |
Aromatic | 0.0 % (0 of 58) | 0.0 % (0 of 29) | 0.0 % (0 of 29) | |
Methyl | 92.9 % (195 of 210) | 92.4 % (97 of 105) | 93.3 % (98 of 105) |
1. entity 1
AALVAHVTSG SGGSGGSGGS GRDLRAELPL TLEEAFHGGE RVVEVAGRRV SVRIPPGVRE GSVIRVPGMG GQGNPPGDLL LVVRLLPHPV FRLEGQDLYA TLDVPAPIAV VGGKVRAMTL EGPVEVAVPP RTQAGRKLRL KGKGFPGPAG RGDLYLEVRI TSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 1 mM [ILVMAT-sel-1H-13C_methyls; U-15N; U-2H] C-CBD1-CBD2, 75 mM potassium chloride, 20 mM potassium phosphate, 0.04 % sodium azide, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | C-CBD1-CBD2 | [ILVMAT-sel-1H-13C_methyls; U-15N; U-2H] | 1 mM | |
2 | potassium chloride | natural abundance | 75 mM | |
3 | potassium phosphate | natural abundance | 20 mM | |
4 | sodium azide | natural abundance | 0.04 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 1 mM [U-100% 13C; U-100% 15N] C-CBD1-CBD2, 75 mM potassium chloride, 20 mM potassium phosphate, 0.04 % sodium azide, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | C-CBD1-CBD2 | [U-100% 13C; U-100% 15N] | 1 mM | |
6 | potassium chloride | natural abundance | 75 mM | |
7 | potassium phosphate | natural abundance | 20 mM | |
8 | sodium azide | natural abundance | 0.04 % |