Solution NMR Structure Of The Full Length Latent Form MinE Protein From Neisseria gonorrheae
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 54.0 % (524 of 971) | 39.2 % (199 of 508) | 65.7 % (245 of 373) | 88.9 % (80 of 90) |
Backbone | 95.6 % (461 of 482) | 92.0 % (150 of 163) | 97.1 % (233 of 240) | 98.7 % (78 of 79) |
Sidechain | 24.3 % (138 of 567) | 13.6 % (47 of 345) | 42.2 % (89 of 211) | 18.2 % (2 of 11) |
Aromatic | 0.0 % (0 of 26) | 0.0 % (0 of 13) | 0.0 % (0 of 13) | |
Methyl | 15.5 % (18 of 116) | 12.1 % (7 of 58) | 19.0 % (11 of 58) |
1. entity 1
MSLIELLFGQ MQKTATVARD RLQIIIAQER AQEGQTPDYL PTLRKALMEV LSKYVNVSLD NIRISQEKQD GMDVLELNIT LSolvent system 95% H2O/5% D2O, Pressure 1 bar, Temperature 298 K, pH 6.5, Details 1.0 mM U-13C/15N MinE-wt, 95% H2O/5% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | MinE-wt | [U-13C; U-15N] | 1.0 mM | |
2 | potassium phosphate | natural abundance | 25 mM | |
3 | EDTA | natural abundance | 0.5 mM | |
4 | benzamidine chloride | natural abundance | 0.1 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 6.5, Details 1.0 mM U-2H/15N MinE-wt-RDC, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | MinE-wt-RDC | [U-2H; U-15N] | 1.0 mM |
Solvent system 95% H2O/5% D2O, Pressure 1 bar, Temperature 298 K, pH 6.5, Details 1.0 mM U-13C/15N MinE-d10, 95% H2O/5% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | MinE-d10 | [U-13C; U-15N] | 1.0 mM | |
7 | potassium phosphate | natural abundance | 25 mM | |
8 | EDTA | natural abundance | 0.5 mM | |
9 | benzamidine chloride | natural abundance | 0.1 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 6.5, Details 1.0 mM U-2H/15N MinE-d10-RDC, 1.0 mM U-2H/15N MinE-d10-CPMG, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
10 | MinE-d10-RDC | [U-2H; U-15N] | 1.0 mM | |
11 | MinE-d10-CPMG | [U-2H; U-15N] | 1.0 mM |