BMRB: 30665

NMR assignment and RNA structure of 5' UTR region stem loop from West Nile Virus

Authors

Sharma, S., Varani, G.

Assembly

5' UTR region stem loop

Entity

1. 5' UTR region stem loop (polymer, Thiol state: not present), 37 monomers, 11904.03 Da Detail

GGUUUCUUAG CACGAAGAUC UCGAUGUCUA AGAAACC

Formula weight

11904.03 Da

Source organism

West Nile virus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.6 %, Completeness: 85.4 %, Completeness (bb): 87.7 % Detail

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Polymer type: polyribonucleotide

	Total	1H	13C	15N
All	85.4 % (505 of 591)	86.0 % (277 of 322)	85.7 % (215 of 251)	72.2 % (13 of 18)
Suger, PO4	87.7 % (357 of 407)	89.6 % (199 of 222)	85.4 % (158 of 185)
Nucleobase	80.4 % (148 of 184)	78.0 % (78 of 100)	86.4 % (57 of 66)	72.2 % (13 of 18)
Aromatic	83.3 % (140 of 168)	83.3 % (70 of 84)	86.4 % (57 of 66)	72.2 % (13 of 18)

1. entity 1

GGUUUCUUAG CACGAAGAUC UCGAUGUCUA AGAAACC

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 0.5 mM [U-99% 13C; U-99% 15N] WNV, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-99% 13C; U-99% 15N]		0.5 (±0.1) mM
2	sodium phosphate	natural abundance		20 mM

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Release date

2019-09-05

Citation

NMR structure of Dengue West Nile viruses stem-loop B: A key cis-acting element for flavivirus replication
Sharma, S., Varani, G.
Biochem. Biophys. Res. Commun. (2020), 531, 522-527, PubMed 32807496 , DOI 10.1016/j.bbrc.2020.07.115 , Abstract

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. 5' UTR region stem loop, : 1 entities Detail

Experiments performed 5 experiments Detail

Chemical shift validation 3 contents Detail