BMRB: 30673

Backbone-modified variant of zinc finger 2 from the transcription factor Sp1 DNA binding domain: BTD in the metal-binding turn

Authors

Rao, S.R., Horne, W.S.

Assembly

Transcription factor Sp1

Entity

1. Transcription factor Sp1, entity 1 (polymer, Thiol state: all other bound), 32 monomers, 3775.331 Da Detail

RPFXCTWXCC GKRFTRSDEL QRHKRTHTGE KX

2. Transcription factor Sp1, entity ZN (non-polymer), 65.409 Da

Total weight

3840.74 Da

Max. entity weight

3775.331 Da

Entity Connection

na 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS5:SG	2:ZN1:ZN
2	na	sing	1:CYS10:SG	2:ZN1:ZN
3	na	sing	1:HIS23:NE2	2:ZN1:ZN
4	na	sing	1:HIS27:NE2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.6 %, Completeness: 95.8 %, Completeness (bb): 96.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	95.8 % (183 of 191)	95.8 % (183 of 191)
Backbone	96.6 % (57 of 59)	96.6 % (57 of 59)
Sidechain	95.5 % (126 of 132)	95.5 % (126 of 132)
Aromatic	95.0 % (19 of 20)	95.0 % (19 of 20)
Methyl	100.0 % (6 of 6)	100.0 % (6 of 6)

1. entity 1

RPFXCTWXCC GKRFTRSDEL QRHKRTHTGE KX

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6, Details 1.2 mM zinc finger 2 from transcription factor Sp1 DNA-binding domain, BTD turn variant, 10 mM [U-2H] TRIS, 0.05 mM DSS, 1.8 mM zinc chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	natural abundance		1.2 mM
2	TRIS	[U-2H]		10 mM
3	DSS	natural abundance		0.05 mM
4	zinc chloride	natural abundance		1.8 mM

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Protein Blocks Logo

Calculated from 10 models in PDB: 6UCO, Strand ID: A Detail

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Release date

2019-09-19

Citation

Proteomimetic Zinc Finger Domains with Modified Metal-binding β-Turns
Rao, S.R., Horne, W.S.
Pept. Sci (Hoboken). (2020), 112, e24177-e24177, PubMed 33733039 , DOI 10.1002/pep2.24177 , Abstract

Related entities 1. Transcription factor Sp1, entity 1, : 1 : 29 : 649 entities Detail

Interaction partners 1. Transcription factor Sp1, entity 1, : 66 interactors Detail

Experiments performed 3 experiments Detail

Chemical shift validation 4 contents Detail