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BMRB: 30680

6URS

Sleeping Beauty transposase PAI subdomain mutant - H19Y

Authors

Nesmelova, I.V., Leighton, G.O., Yan, C., Lustig, J., Corona, R.I., Guo, J.T., Ivics, Z.

Assembly

BAH domain-containing protein

Entity

1. BAH domain-containing protein (polymer, Thiol state: not present), 57 monomers, 6373.270 Da Detail

ASMGKSKEIS QDLRKRIVDL YKSGSSLGAI SKRLAVPRSS VQTIVRKYKH HGTTQHH

Formula weight

6373.27 Da

Source organism

Oncorhynchus mykiss

Exptl. method

Refine. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.7 %, Completeness: 71.9 %, Completeness (bb): 75.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	71.9 % (484 of 673)	82.4 % (290 of 352)	54.1 % (139 of 257)	85.9 % (55 of 64)
Backbone	75.6 % (257 of 340)	92.3 % (108 of 117)	58.1 % (97 of 167)	92.9 % (52 of 56)
Sidechain	71.2 % (275 of 386)	77.4 % (182 of 235)	62.9 % (90 of 143)	37.5 % (3 of 8)
Aromatic	25.0 % (8 of 32)	50.0 % (8 of 16)	0.0 % (0 of 16)
Methyl	98.3 % (59 of 60)	100.0 % (30 of 30)	96.7 % (29 of 30)

1. entity 1

ASMGKSKEIS QDLRKRIVDL YKSGSSLGAI SKRLAVPRSS VQTIVRKYKH HGTTQHH

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 278 K, pH 5, Details 0.5 mM [U-13C; U-15N] pai subdomain mutant, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	pai subdomain mutant	[U-13C; U-15N]		0.5 mM

Protein Blocks Logo

Calculated from 8 models in PDB: 6URS, Strand ID: A Detail

Release date

2019-11-24

Citation

H19Y mutation in the primary DNA-recognition subdomain of the Sleeping Beauty transposase improves structural stability, transposon DNA-binding and transposition
Nesmelova, I.V., Leighton, G.O., Yan, C., Lustig, J., Corona, R.I., Guo, J.T., Ivics, Z.

Related entities 1. BAH domain-containing protein, : 1 : 20 entities Detail

Experiments performed 8 experiments Detail

1 3D HNCACB

Instrument

Bruker AVANCE III - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 278 K, pH 5, Details 0.5 mM [U-13C; U-15N] pai subdomain mutant, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	pai subdomain mutant	[U-13C; U-15N]		0.5 mM

2 3D CBCA(CO)NH

Instrument

Bruker AVANCE III - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 278 K, pH 5, Details 0.5 mM [U-13C; U-15N] pai subdomain mutant, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	pai subdomain mutant	[U-13C; U-15N]		0.5 mM

3 3D 1H-15N TOCSY

Instrument

Bruker AVANCE III - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 278 K, pH 5, Details 0.5 mM [U-13C; U-15N] pai subdomain mutant, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	pai subdomain mutant	[U-13C; U-15N]		0.5 mM

4 3D 1H-15N NOESY

Instrument

Bruker AVANCE III - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 278 K, pH 5, Details 0.5 mM [U-13C; U-15N] pai subdomain mutant, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	pai subdomain mutant	[U-13C; U-15N]		0.5 mM

5 3D 1H-13C NOESY

Instrument

Bruker AVANCE III - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 278 K, pH 5, Details 0.5 mM [U-13C; U-15N] pai subdomain mutant, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	pai subdomain mutant	[U-13C; U-15N]		0.5 mM

6 2D 1H-15N HSQC

Instrument

Bruker AVANCE III - 950 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 5, Details 0.5 mM [U-13C; U-15N] pai subdomain mutant, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	pai subdomain mutant	[U-13C; U-15N]		0.5 mM

7 3D CBCA(CO)NH

Instrument

Bruker AVANCE III - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 5, Details 0.5 mM [U-13C; U-15N] pai subdomain mutant, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	pai subdomain mutant	[U-13C; U-15N]		0.5 mM

8 3D HNCACB

Instrument

Bruker AVANCE III - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 5, Details 0.5 mM [U-13C; U-15N] pai subdomain mutant, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	pai subdomain mutant	[U-13C; U-15N]		0.5 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr30680_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr30680_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	558		94.7 (chain: 1, length: 57)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 57, Sequence coverage: 94.7%
- Total number of assignments
  - ¹H chemical shifts: 360
  - ¹³C chemical shifts: 143
  - ¹⁵N chemical shifts: 55
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Keywords DNA BINDING PROTEIN, transposase