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Found 1 Document (0.562 seconds)

BMRB: 30684

6UZJ

NMR structure of the HACS1 SH3 domain

Authors

Donaldson, L.W.

Assembly

SAM domain-containing protein SAMSN-1

Entity

1. SAM domain-containing protein SAMSN-1 (polymer, Thiol state: all free), 87 monomers, 9644.885 Da Detail

GSDITSLYKK AGSSFRLDDD GPYSGPFCGR ARVHTDFTPS PYDTDSLKIK KGDIIDIICK TPMGMWTGML NNKVGNFKFI YVDVISE

Formula weight

9644.885 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 78.2 %, Completeness: 71.7 %, Completeness (bb): 73.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	71.7 % (720 of 1004)	71.6 % (373 of 521)	70.5 % (280 of 397)	77.9 % (67 of 86)
Backbone	73.8 % (378 of 512)	74.2 % (132 of 178)	72.6 % (183 of 252)	76.8 % (63 of 82)
Sidechain	70.4 % (401 of 570)	70.3 % (241 of 343)	70.0 % (156 of 223)	100.0 % (4 of 4)
Aromatic	55.1 % (54 of 98)	59.2 % (29 of 49)	50.0 % (24 of 48)	100.0 % (1 of 1)
Methyl	82.5 % (66 of 80)	80.0 % (32 of 40)	85.0 % (34 of 40)

1. entity 1

GSDITSLYKK AGSSFRLDDD GPYSGPFCGR ARVHTDFTPS PYDTDSLKIK KGDIIDIICK TPMGMWTGML NNKVGNFKFI YVDVISE

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.7, Details 20 mM sodium phosphate, 150 mM sodium chloride, 0.05 % w/v sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	entity_1	[U-99% 13C; U-99% 15N]	0.6 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	150 mM
4	sodium azide	natural abundance	0.05 % w/v

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.7, Details 20 mM sodium phosphate, 150 mM sodium chloride, 0.05 % w/v sodium azide, 10 mg/mL Pf1 phage, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
5	entity_1	[U-99% 13C; U-99% 15N]	0.6 mM
6	sodium phosphate	natural abundance	20 mM
7	sodium chloride	natural abundance	150 mM
8	sodium azide	natural abundance	0.05 % w/v
9	Pf1 phage	natural abundance	10 mg/mL

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.25 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.25 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.65 ppm, Outliers: 1 Detail

Release date

2019-11-18

Citation

HACS1 signaling adaptor protein recognizes a motif in the paired immunoglobulin receptor B cytoplasmic domain
Kwan, J.J., Slavkovic, S., Piazza, M., Wang, D., Dieckmann, T., Johnson, P.E., Wen, X.Y., Donaldson, L.W.
Commun. Biol. (2020), 3, 672-672, PubMed 33188360 , DOI 10.1038/s42003-020-01397-z , Abstract

Related entities 1. SAM domain-containing protein SAMSN-1, : 1 : 3 : 29 entities Detail

Interaction partners 1. SAM domain-containing protein SAMSN-1, : 2 interactors Detail

More details for 2 interactors identified by 2 citations