BMRB: 30714

Solution NMR structure of Prochlorosin 2.1 produced by Prochlorococcus MIT 9313

Authors

Bobeica, S.C., van der Donk, W.A., Zhu, L.

Assembly

Prochlorosin 2.1

Entity

1. Prochlorosin 2.1 (polymer, Thiol state: not reported), 28 monomers, 2759.127 Da Detail

CCIXGESPGX APXNDYKCXK GRGPGGCY

Formula weight

2759.127 Da

Source organism

Prochlorococcus marinus str. MIT 9313

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 85.7 %, Completeness: 90.8 %, Completeness (bb): 98.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	90.8 % (118 of 130)	90.8 % (118 of 130)
Backbone	98.0 % (50 of 51)	98.0 % (50 of 51)
Sidechain	86.1 % (68 of 79)	86.1 % (68 of 79)
Aromatic	100.0 % (8 of 8)	100.0 % (8 of 8)
Methyl	100.0 % (3 of 3)	100.0 % (3 of 3)

1. entity 1

CCIXGESPGX APXNDYKCXK GRGPGGCY

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 (±0) atm, Temperature 296 (±0.1) K, pH 6 (±0.1), Details 2 mM Prochlorosin 2.1, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Prochlorosin 2.1	natural abundance		2 (±0.3) mM

Sample #2

Solvent system 100% D2O, Pressure 1 (±0) atm, Temperature 296 (±0.1) K, pH 6 (±0.1), Details 3 mM Prochlorosin 2.1, 100% D2O

#	Name	Isotope labeling	Type	Concentration
2	Prochlorosin 2.1	natural abundance		3 (±0.3) mM

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Protein Blocks Logo

Calculated from 20 models in PDB: 6VJQ, Strand ID: A Detail

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Release date

2020-01-20

Citation 1

Evolutionary radiation of lanthipeptides in marine cyanobacteria
Cubillos-Ruiz, A., Berta-Thompson, J.W., Becker, J.W., van der Donk, W.A., Chisholm, S.W.
Proc. Natl. Acad. Sci. U. S. A. (2017), 114, E5424-E5433, PubMed 28630351 , DOI 10.1073/pnas.1700990114 , Abstract

Show more 2 citaions

Citation 2

Structural characterization of four prochlorosins: a novel class of lantipeptides produced by planktonic marine cyanobacteria
Tang, W., van der Donk, W.A.
Biochemistry (2012), 51, 4271-4279, PubMed 22574919 , DOI 10.1021/bi300255s , Abstract

Citation 3

Catalytic promiscuity in the biosynthesis of cyclic peptide secondary metabolites in planktonic marine cyanobacteria
Li, B., Sher, D., Kelly, L., Shi, Y., Huang, K., Knerr, P.J., Joewono, I., Rusch, D., Chisholm, S.W., van der Donk, W.A.
Proc. Natl. Acad. Sci. U. S. A. (2010), 107, 10430-10435, PubMed 20479271 , DOI 10.1073/pnas.0913677107 , Abstract

Hide non-primary 2 citaions

Related entities 1. Prochlorosin 2.1, : 1 entities Detail

Experiments performed 4 experiments Detail

Chemical shift validation 3 contents Detail