BMRB: 30724

Cap1G-TPUA

Authors

Summers, M.F., Brown, J.D.

Assembly

RNA (130-MER)

Entity

1. RNA (130-MER) (polymer, Thiol state: not present), 130 monomers, 41986.74 Da Detail

XGUCUCUCUU GUUAGACCAG GUCGAGCCCG GGAGCUCUCU GGCUAGCAAG GAACCCACUG CUUAAGCCUC AAUAAAGCUU GCCUUGAGUG CCUCAAGCAG UGUGUGCCCA UCUCGAGAGA GAUGGGUGCG

Formula weight

41986.74 Da

Source organism

Human immunodeficiency virus 1

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.2 %, Completeness: 54.3 %, Completeness (bb): 50.3 % Detail

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Polymer type: polyribonucleotide

	Total	1H
All	54.3 % (615 of 1132)	54.3 % (615 of 1132)
Suger, PO4	50.3 % (389 of 774)	50.3 % (389 of 774)
Nucleobase	63.1 % (226 of 358)	63.1 % (226 of 358)
Aromatic	77.8 % (224 of 288)	77.8 % (224 of 288)

1. entity 1

XGUCUCUCUU GUUAGACCAG GUCGAGCCCG GGAGCUCUCU GGCUAGCAAG GAACCCACUG CUUAAGCCUC AAUAAAGCUU GCCUUGAGUG CCUCAAGCAG UGUGUGCCCA UCUCGAGAGA GAUGGGUGCG

Show sample condition

Sample #1

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 7.4, Details 150 uM 1H Cap1G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 mM KCl, 100% D2O

#	Name	Isotope labeling	Type	Concentration
1	Cap1G-TPUA	1H		150 uM
2	KH2PO4	Deuterated		10 mM
3	MgCl2	natural abundance		1 mM
4	KCl	natural abundance		122 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 7.4, Details 150 uM Gh Cap1G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 mM KCl, 100% D2O

#	Name	Isotope labeling	Type	Concentration
5	Cap1G-TPUA	Gh		150 uM
6	KH2PO4	Deuterated		10 mM
7	MgCl2	natural abundance		1 mM
8	KCl	natural abundance		122 mM

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 7.4, Details 150 uM A2rGr Cap1G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 mM KCl, 100% D2O

#	Name	Isotope labeling	Type	Concentration
9	Cap1G-TPUA	A2rGr		150 uM
10	KH2PO4	Deuterated		10 mM
11	MgCl2	natural abundance		1 mM
12	KCl	natural abundance		122 mM

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 7.4, Details 150 uM GhU6r Cap1G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 mM KCl, 100% D2O

#	Name	Isotope labeling	Type	Concentration
13	Cap1G-TPUA	GhU6r		150 uM
14	KH2PO4	Deuterated		10 mM
15	MgCl2	natural abundance		1 mM
16	KCl	natural abundance		122 mM

Sample #5

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 7.4, Details 150 uM U6r Cap1G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 mM KCl, 100% D2O

#	Name	Isotope labeling	Type	Concentration
17	Cap1G-TPUA	U6r		150 uM
18	KH2PO4	Deuterated		10 mM
19	MgCl2	natural abundance		1 mM
20	KCl	natural abundance		122 mM

Sample #6

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 7.4, Details 150 uM C6r Cap1G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 mM KCl, 100% D2O

#	Name	Isotope labeling	Type	Concentration
21	Cap1G-TPUA	C6r		150 uM
22	KH2PO4	Deuterated		10 mM
23	MgCl2	natural abundance		1 mM
24	KCl	natural abundance		122 mM

Sample #7

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 7.4, Details 150 uM C6rU6r Cap1G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 mM KCl, 100% D2O

#	Name	Isotope labeling	Type	Concentration
25	Cap1G-TPUA	C6rU6r		150 uM
26	KH2PO4	Deuterated		10 mM
27	MgCl2	natural abundance		1 mM
28	KCl	natural abundance		122 mM

Sample #8

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 7.4, Details 150 uM AhCh Cap1G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 mM KCl, 100% D2O

#	Name	Isotope labeling	Type	Concentration
29	Cap1G-TPUA	AhCh		150 uM
30	KH2PO4	Deuterated		10 mM
31	MgCl2	natural abundance		1 mM
32	KCl	natural abundance		122 mM

Sample #9

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7.4, Details 150 uM U6r Cap1G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 mM KCl, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
33	Cap1G-TPUA	U6r		150 uM
34	KH2PO4	Deuterated		10 mM
35	MgCl2	natural abundance		1 mM
36	KCl	natural abundance		122 mM

Sample #10

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7.4, Details 150 uM 1H Cap1G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 mM KCl, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
37	Cap1G-TPUA	1H		150 uM
38	KH2PO4	Deuterated		10 mM
39	MgCl2	natural abundance		1 mM
40	KCl	natural abundance		122 mM

Sample #11

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7.4, Details 150 uM Gh Cap1G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 mM KCl, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
41	Cap1G-TPUA	Gh		150 uM
42	KH2PO4	Deuterated		10 mM
43	MgCl2	natural abundance		1 mM
44	KCl	natural abundance		122 mM

Sample #12

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 7.4, Details 150 uM 1H 2G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 mM KCl, 100% D2O

#	Name	Isotope labeling	Type	Concentration
45	KH2PO4	Deuterated		10 mM
46	MgCl2	natural abundance		1 mM
47	KCl	natural abundance		122 mM
48	2G-TPUA	1H		150 uM

Sample #13

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 7.4, Details 150 uM Ah 2G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 mM KCl, 100% D2O

#	Name	Isotope labeling	Type	Concentration
49	KH2PO4	Deuterated		10 mM
50	MgCl2	natural abundance		1 mM
51	KCl	natural abundance		122 mM
52	2G-TPUA	Ah		150 uM

Sample #14

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 7.4, Details 150 uM Ch 2G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 mM KCl, 100% D2O

#	Name	Isotope labeling	Type	Concentration
53	KH2PO4	Deuterated		10 mM
54	MgCl2	natural abundance		1 mM
55	KCl	natural abundance		122 mM
56	2G-TPUA	Ch		150 uM

Sample #15

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 7.4, Details 150 uM Uh 2G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 mM KCl, 100% D2O

#	Name	Isotope labeling	Type	Concentration
57	KH2PO4	Deuterated		10 mM
58	MgCl2	natural abundance		1 mM
59	KCl	natural abundance		122 mM
60	2G-TPUA	Uh		150 uM

Sample #16

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 7.4, Details 150 uM A2rCr 2G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 mM KCl, 100% D2O

#	Name	Isotope labeling	Type	Concentration
61	KH2PO4	Deuterated		10 mM
62	MgCl2	natural abundance		1 mM
63	KCl	natural abundance		122 mM
64	2G-TPUA	A2rCr		150 uM

Sample #17

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 7.4, Details 150 uM A2rUr 2G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 mM KCl, 100% D2O

#	Name	Isotope labeling	Type	Concentration
65	KH2PO4	Deuterated		10 mM
66	MgCl2	natural abundance		1 mM
67	KCl	natural abundance		122 mM
68	2G-TPUA	A2rUr		150 uM

Sample #18

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 7.4, Details 150 uM C6rUr 2G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 mM KCl, 100% D2O

#	Name	Isotope labeling	Type	Concentration
69	KH2PO4	Deuterated		10 mM
70	MgCl2	natural abundance		1 mM
71	KCl	natural abundance		122 mM
72	2G-TPUA	C6rUr		150 uM

Sample #19

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 7.4, Details 150 uM CrU6r 2G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 mM KCl, 100% D2O

#	Name	Isotope labeling	Type	Concentration
73	KH2PO4	Deuterated		10 mM
74	MgCl2	natural abundance		1 mM
75	KCl	natural abundance		122 mM
76	2G-TPUA	CrU6r		150 uM

Sample #20

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 7.4, Details 150 uM AhCh 2G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 mM KCl, 100% D2O

#	Name	Isotope labeling	Type	Concentration
77	KH2PO4	Deuterated		10 mM
78	MgCl2	natural abundance		1 mM
79	KCl	natural abundance		122 mM
80	2G-TPUA	AhCh		150 uM

Sample #21

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 7.4, Details 150 uM ChGh 2G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 mM KCl, 100% D2O

#	Name	Isotope labeling	Type	Concentration
81	KH2PO4	Deuterated		10 mM
82	MgCl2	natural abundance		1 mM
83	KCl	natural abundance		122 mM
84	2G-TPUA	ChGh		150 uM

Sample #22

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 7.4, Details 150 uM GhUh 2G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 mM KCl, 100% D2O

#	Name	Isotope labeling	Type	Concentration
85	KH2PO4	Deuterated		10 mM
86	MgCl2	natural abundance		1 mM
87	KCl	natural abundance		122 mM
88	2G-TPUA	GhUh		150 uM

Sample #23

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 7.4, Details 150 uM AhUh 2G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 mM KCl, 100% D2O

#	Name	Isotope labeling	Type	Concentration
89	KH2PO4	Deuterated		10 mM
90	MgCl2	natural abundance		1 mM
91	KCl	natural abundance		122 mM
92	2G-TPUA	AhUh		150 uM

Sample #24

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 7.4, Details 150 uM AhGh 2G-TPUA, 10 mM Deuterated KH2PO4, 1 mM MgCl2, 122 M KCl, 100% D2O

#	Name	Isotope labeling	Type	Concentration
93	KH2PO4	Deuterated		10 mM
94	MgCl2	natural abundance		1 mM
95	KCl	natural abundance		122 M
96	2G-TPUA	AhGh		150 uM

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Release date

2020-04-06

Citation

Structural basis for transcriptional start site control of HIV-1 RNA fate
Brown, J.D., Kharytonchyk, S., Chaudry, I., Iyer, A.S., Carter, H., Becker, G., Desai, Y., Glang, L., Choi, S.H., Singh, K., Lopresti, M.W., Orellana, M., Rodriguez, T., Oboh, U., Hijji, J., Ghinger, F.G., Stewart, K., Francis, D., Edwards, B., Chen, P., Case, D.A., Telesnitsky, A., Summers, M.F.
Science (2020), 368, 413-417, PubMed 32327595 , DOI 10.1126/science.aaz7959 , Abstract

Related entities 1. RNA (130-MER), : 1 entities Detail

Experiments performed 24 experiments Detail