BMRBj - BMREntryMaster

Found 1 Document (0.55 seconds)

BMRB: 30737

6WA1

Dimeric form of the trans-stabilized Hemolysin II C-terminal domain

Authors

Kaplan, A.R., Alexandrescu, A.T.

Assembly

Hemolysin II

Entity

1. Hemolysin II (polymer, Thiol state: not present), 94 monomers, 10449.60 × 2 Da Detail

DNQKALEEQM NSINSVNDKL NKGKGKLSLS MNGNQLKATS SNAGYGISYE DKNWGIFVNG EKVYTFNEKS TVGNISNDIN KLNIKGMYIE IKQI

Total weight

20899.2 Da

Max. entity weight

10449.6 Da

Source organism

Bacillus cereus ATCC 14579

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.9 %, Completeness: 38.4 %, Completeness (bb): 75.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	38.4 % (426 of 1110)	15.9 % (93 of 584)	58.5 % (241 of 412)	80.7 % (92 of 114)
Backbone	75.5 % (426 of 564)	47.2 % (93 of 197)	88.3 % (241 of 273)	97.9 % (92 of 94)
Sidechain	13.2 % (83 of 631)	0.0 % (0 of 387)	37.1 % (83 of 224)	0.0 % (0 of 20)
Aromatic	0.0 % (0 of 64)	0.0 % (0 of 32)	0.0 % (0 of 31)	0.0 % (0 of 1)
Methyl	2.3 % (2 of 88)	0.0 % (0 of 44)	4.5 % (2 of 44)

1. entity 1

DNQKALEEQM NSINSVNDKL NKGKGKLSLS MNGNQLKATS SNAGYGISYE DKNWGIFVNG EKVYTFNEKS TVGNISNDIN KLNIKGMYIE IKQI

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303.15 K, pH 6.5, Details 0.8 mM [U-99% 13C; U-99% 15N] HlyIIC, 20 mM sodium phosphate, 1 mM EDTA, 0.05 % w/v sodium azide, 1 mM AEBSF protease inhibitor, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	HlyIIC	[U-99% 13C; U-99% 15N]	0.8 mM
2	sodium phosphate	natural abundance	20 mM
3	EDTA	natural abundance	1 mM
4	sodium azide	natural abundance	0.05 % w/v
5	AEBSF protease inhibitor	natural abundance	1 mM

Release date

2020-04-13

Citation 1

NMR structure of the Bacillus cereus hemolysin II C-terminal domain reveals a novel fold
Kaplan, A.R., Kaus, K., De, S., Olson, R., Alexandrescu, A.T.
Sci. Rep. (2017), 7, 3277-3277, PubMed 28607368 , DOI 10.1038/s41598-017-02917-4 , Abstract

Show more 2 citaions

Citation 2

NMR assignments for the cis and trans forms of the hemolysin II C-terminal domain
Maciejewski, M.W., Olson, R., Alexandrescu, A.T., Kaplan, A.R.
Biomol. NMR Assign. (2014), 8, 419-423, PubMed 24234348 , DOI 10.1007/s12104-013-9530-2 , Abstract

Citation 3

Protein yoga: Conformational versatility of the Hemolysin II C-terminal domain detailed by NMR structures for multiple states
Kaplan, A.R., Olson, R., Alexandrescu, A.T.
Protein Sci. (2021), 30, 990-1005, PubMed 33733504 , DOI 10.1002/pro.4066 , Abstract

Hide non-primary 2 citaions

Related entities 1. Hemolysin II, : 1 : 1 : 2 entities Detail

Experiments performed 7 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303.15 K, pH 6.5, Details 0.8 mM [U-99% 13C; U-99% 15N] HlyIIC, 20 mM sodium phosphate, 1 mM EDTA, 0.05 % w/v sodium azide, 1 mM AEBSF protease inhibitor, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	HlyIIC	[U-99% 13C; U-99% 15N]	0.8 mM
2	sodium phosphate	natural abundance	20 mM
3	EDTA	natural abundance	1 mM
4	sodium azide	natural abundance	0.05 % w/v
5	AEBSF protease inhibitor	natural abundance	1 mM

2 3D HNCACB

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	HlyIIC	[U-99% 13C; U-99% 15N]	0.8 mM
2	sodium phosphate	natural abundance	20 mM
3	EDTA	natural abundance	1 mM
4	sodium azide	natural abundance	0.05 % w/v
5	AEBSF protease inhibitor	natural abundance	1 mM

3 3D HNCO

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	HlyIIC	[U-99% 13C; U-99% 15N]	0.8 mM
2	sodium phosphate	natural abundance	20 mM
3	EDTA	natural abundance	1 mM
4	sodium azide	natural abundance	0.05 % w/v
5	AEBSF protease inhibitor	natural abundance	1 mM

4 3D 1H-15N NOESY

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	HlyIIC	[U-99% 13C; U-99% 15N]	0.8 mM
2	sodium phosphate	natural abundance	20 mM
3	EDTA	natural abundance	1 mM
4	sodium azide	natural abundance	0.05 % w/v
5	AEBSF protease inhibitor	natural abundance	1 mM

5 3D 1H-15N TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	HlyIIC	[U-99% 13C; U-99% 15N]	0.8 mM
2	sodium phosphate	natural abundance	20 mM
3	EDTA	natural abundance	1 mM
4	sodium azide	natural abundance	0.05 % w/v
5	AEBSF protease inhibitor	natural abundance	1 mM

6 3D 1H-13C NOESY (edited)

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	HlyIIC	[U-99% 13C; U-99% 15N]	0.8 mM
2	sodium phosphate	natural abundance	20 mM
3	EDTA	natural abundance	1 mM
4	sodium azide	natural abundance	0.05 % w/v
5	AEBSF protease inhibitor	natural abundance	1 mM

7 3D 1H-13C NOESY (filtered)

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	HlyIIC	[U-99% 13C; U-99% 15N]	0.8 mM
2	sodium phosphate	natural abundance	20 mM
3	EDTA	natural abundance	1 mM
4	sodium azide	natural abundance	0.05 % w/v
5	AEBSF protease inhibitor	natural abundance	1 mM

nullKeywords TOXIN, domain swapped dimer

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (0.55 seconds)

BMRB: 30737

Sample

Related entities 1. Hemolysin II, : 1 : 1 : 2 entities Detail

Experiments performed 7 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample