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Atomic-Resolution Structure of HIV-1 Capsid Tubes by Magic Angle Spinning NMR
Authors
Lu, M., Russell, R.W., Bryer, A., Quinn, C.M., Hou, G., Zhang, H., Schwieters, C.D., Perilla, J.R., Gronenborn, A.M., Polenova, T.
Assembly
HIV-1 capsid protein
Entity
1. HIV-1 capsid protein (polymer), 231 monomers, 25602.16 Da Detail

PIVQNLQGQM VHQAISPRTL NAWVKVVEEK AFSPEVIPMF SALSEGATPQ DLNTMLNTVG GHQAAMQMLK ETINEEAAEW DRLHPVHAGP IAPGQMREPR GSDIAGTTST LQEQIGWMTH NPPIPVGEIY KRWIILGLNK IVRMYSPTSI LDIRQGPKEP FRDYVDRFYK TLRAEQASQE VKNWMTETLL VQNANPDCKT ILKALGPGAT LEEMMTACQG VGGPGHKARV L


Formula weight
25602.16 Da
Source organism
Human immunodeficiency virus 1
Exptl. method
solid-state NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 97.0 %, Completeness: 83.4 %, Completeness (bb): 92.1 % Detail

Polymer type: polypeptide(L)

Total13C15N
All83.4 % (1077 of 1292)84.4 % (876 of 1038)79.1 % (201 of 254)
Backbone92.1 % (818 of 888)91.4 % (617 of 675)94.4 % (201 of 213)
Sidechain75.0 % (463 of 617)80.4 % (463 of 576) 0.0 % (0 of 41)
Aromatic51.3 % (40 of 78)54.8 % (40 of 73) 0.0 % (0 of 5)
Methyl85.5 % (112 of 131)85.5 % (112 of 131)

1. entity 1

PIVQNLQGQM VHQAISPRTL NAWVKVVEEK AFSPEVIPMF SALSEGATPQ DLNTMLNTVG GHQAAMQMLK ETINEEAAEW DRLHPVHAGP IAPGQMREPR GSDIAGTTST LQEQIGWMTH NPPIPVGEIY KRWIILGLNK IVRMYSPTSI LDIRQGPKEP FRDYVDRFYK TLRAEQASQE VKNWMTETLL VQNANPDCKT ILKALGPGAT LEEMMTACQG VGGPGHKARV L

Sample #1

Solvent system solid, Pressure 1 atm, Temperature 277 K, pH 6, Details 100 % [U-13C; U-15N] HIV-1 capsid protein, solid


#NameIsotope labelingTypeConcentration
1HIV-1 capsid protein[U-13C; U-15N]100 %
Sample #2

Solvent system solid, Pressure 1 atm, Temperature 277 K, pH 6, Details 100 % [1,6-13C]-Glucose,U-15N HIV-1 capsid protein, solid


#NameIsotope labelingTypeConcentration
2HIV-1 capsid protein[1,6-13C]-Glucose; U-15N100 %
Sample #3

Solvent system solid, Pressure 1 atm, Temperature 277 K, pH 6, Details 100 % [2-13C]-Glucose,U-15N HIV-1 capsid protein, solid


#NameIsotope labelingTypeConcentration
3HIV-1 capsid protein[2-13C]-Glucose; U-15N100 %
Sample #4

Solvent system solid, Pressure 1 atm, Temperature 277 K, pH 6, Details 100 % 13C,15N-His HIV-1 capsid protein, solid


#NameIsotope labelingTypeConcentration
4HIV-1 capsid protein[13C,15N-His]100 %
Sample #5

Solvent system solid, Pressure 1 atm, Temperature 277 K, pH 6, Details 100 % 13C,15N-Tyr HIV-1 capsid protein, solid


#NameIsotope labelingTypeConcentration
5HIV-1 capsid protein[13C,15N-Tyr]100 %
Sample #6

Solvent system solid, Pressure 1 atm, Temperature 277 K, pH 6, Details 50 % 13C,15N-Ala HIV-1 capsid protein, 50 % 13C,15N-Ile HIV-1 capsid protein, solid


#NameIsotope labelingTypeConcentration
613C,15N-Ala HIV-1 capsid protein[13C,15N-Ala]50 %
713C,15N-Ile HIV-1 capsid protein[13C,15N-Ile]50 %
Sample #7

Solvent system solid, Pressure 1 atm, Temperature 277 K, pH 6, Details 50 % 13C,15N-Ala HIV-1 capsid protein, 50 % 13C,15N-Val HIV-1 capsid protein, solid


#NameIsotope labelingTypeConcentration
813C,15N-Ala HIV-1 capsid protein[13C,15N-Ala]50 %
913C,15N-Val HIV-1 capsid protein[13C,15N-Val]50 %
Sample #8

Solvent system solid, Pressure 1 atm, Temperature 277 K, pH 6, Details 86 % HIV-1 capsid protein, 14 % U-13C,15N-CA HIV-1 capsid protein, solid


#NameIsotope labelingTypeConcentration
10HIV-1 capsid proteinnatural abundance86 %
11U-13C,15N-CA HIV-1 capsid protein[U-13C; 15N-CA]14 %
Sample #9

Solvent system solid, Pressure 1 atm, Temperature 277 K, pH 6, Details 50 % [U-13C] HIV-1 capsid protein, 50 % [U-15N] HIV-1 capsid protein, solid


#NameIsotope labelingTypeConcentration
12[U-13C] HIV-1 capsid protein[U-13C]50 %
13[U-15N] HIV-1 capsid protein[U-15N]50 %

Release date
2020-08-06
Citation
Atomic-resolution structure of HIV-1 capsid tubes by magic-angle spinning NMR
Lu, M., Russell, R.W., Bryer, A., Quinn, C.M., Hou, G., Zhang, H., Schwieters, C.D., Perilla, J.R., Gronenborn, A.M., Polenova, T.
Nat. Struct. Mol. Biol. (2020), 27, 863-869, PubMed 32901160 , DOI 10.1038/s41594-020-0489-2 ,
Related entities 1. HIV-1 capsid protein, : 1 : 30 : 2 : 66 : 124 entities Detail
Interaction partners 1. HIV-1 capsid protein, : 4 interactors Detail
Experiments performed 37 experiments Detail
NMR combined restraints 4 contents Detail
Keywords CA protein assemblies, HIV-1 capsid, HIV-AIDS, VIRAL PROTEIN, magic angle spinning NMR