BMRB: 30757

Structure of the C-terminal domain of BCL-XL in membrane

Authors

Yao, Y., Tian, Y., Marassi, F.M.

Assembly

Bcl-2-like protein 1

Entity

1. Bcl-2-like protein 1 (polymer, Thiol state: not present), 28 monomers, 3170.687 Da Detail

GQERFNRWFL TGMTVAGVVL LGSLFSRK

Formula weight

3170.687 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 82.1 %, Completeness: 47.5 %, Completeness (bb): 65.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	47.5 % (161 of 339)	53.7 % (94 of 175)	33.1 % (44 of 133)	74.2 % (23 of 31)
Backbone	65.5 % (110 of 168)	75.0 % (45 of 60)	52.5 % (42 of 80)	82.1 % (23 of 28)
Sidechain	35.9 % (70 of 195)	42.6 % (49 of 115)	27.3 % (21 of 77)	0.0 % (0 of 3)
Aromatic	0.0 % (0 of 42)	0.0 % (0 of 21)	0.0 % (0 of 20)	0.0 % (0 of 1)
Methyl	52.9 % (18 of 34)	88.2 % (15 of 17)	17.6 % (3 of 17)

1. entity 1

GQERFNRWFL TGMTVAGVVL LGSLFSRK

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 318 K, pH 6.5, Details 0.2 mM [U-99% 15N] C-terminal domain of BCL-XL, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	C-terminal domain of BCL-XL	[U-99% 15N]		0.2 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 318 K, pH 6.5, Details 0.2 mM [U-99% 13C; U-99% 15N] C-terminal domain of BCL-XL, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
2	C-terminal domain of BCL-XL	[U-99% 13C; U-99% 15N]		0.2 mM

Sample #3

Solvent system 95% H2O/5% D2O, Pressure 1 bar, Temperature 318 K, pH 6.5, Details 4 mg/mL selectively 15N Leu labeled C-terminal domain of BCL-XL, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
3	C-terminal domain of BCL-XL	[selectively 15N Leu labeled]		4 mg/mL

Sample #4

Solvent system 95% H2O/5% D2O, Pressure 1 bar, Temperature 318 K, pH 6.5, Details 10 mg/mL selectively 15N Leu labeled C-terminal domain of BCL-XL, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
4	C-terminal domain of BCL-XL	[selectively 15N Leu labeled]		10 mg/mL

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Protein Blocks Logo

Calculated from 10 models in PDB: 6X7I, Strand ID: A Detail

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Release date

2020-06-21

Citation

Conformational States of the Cytoprotective Protein Bcl-xL
Ryzhov, P., Tian, Y., Yao, Y., Bobkov, A.A., Im, W., Marassi, F.M.
Biophys. J. (2020), 119, 1324-1334, PubMed 32888404 , DOI 10.1016/j.bpj.2020.08.014 , Abstract

Related entities 1. Bcl-2-like protein 1, : 1 : 9 : 1 : 2 entities Detail

Interaction partners 1. Bcl-2-like protein 1, : 4 interactors Detail

Experiments performed 5 experiments Detail

Chemical shift validation 3 contents Detail