Solution Structure of the Dysferlin C2A Domain in its Calcium-free State
GSGGGGGMLR VFILYAENVH TPDTDISDAY CSAVFAGVKK RTKVIKNSVN PVWNEGFEWD LKGIPLDQGS ELHVVVKDHE TMGRNRFLGE AKVPLREVLA TPSLSASFNA PLLDTKKQPT GASLVLQVSY TPLPGAVL
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 68.4 % (1072 of 1567) | 74.7 % (601 of 805) | 57.5 % (355 of 617) | 80.0 % (116 of 145) |
Backbone | 68.9 % (558 of 810) | 80.8 % (227 of 281) | 56.5 % (226 of 400) | 81.4 % (105 of 129) |
Sidechain | 70.0 % (617 of 881) | 71.4 % (374 of 524) | 68.0 % (232 of 341) | 68.8 % (11 of 16) |
Aromatic | 19.1 % (21 of 110) | 34.5 % (19 of 55) | 0.0 % (0 of 53) | 100.0 % (2 of 2) |
Methyl | 85.5 % (147 of 172) | 87.2 % (75 of 86) | 83.7 % (72 of 86) |
1. entity 1
GSGGGGGMLR VFILYAENVH TPDTDISDAY CSAVFAGVKK RTKVIKNSVN PVWNEGFEWD LKGIPLDQGS ELHVVVKDHE TMGRNRFLGE AKVPLREVLA TPSLSASFNA PLLDTKKQPT GASLVLQVSY TPLPGAVLSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298.15 K, pH 5.5, Details 1 mM DSS, 25 mM MES, 150 mM NaCl, 5 mM EDTA, 1 mM DTT, 0.3 mM [U-13C; U-15N] Protein, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | DSS | natural abundance | 1 mM | |
2 | MES | natural abundance | 25 mM | |
3 | NaCl | natural abundance | 150 mM | |
4 | EDTA | natural abundance | 5 mM | |
5 | DTT | natural abundance | 1 mM | |
6 | Protein | [U-13C; U-15N] | 0.3 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298.15 K, pH 5.5, Details 1 mM DSS, 25 mM MES, 150 mM NaCl, 5 mM EDTA, 1 mM DTT, 0.3 mM [U-13C; U-15N] Protein, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | DSS | natural abundance | 1 mM | |
8 | MES | natural abundance | 25 mM | |
9 | NaCl | natural abundance | 150 mM | |
10 | EDTA | natural abundance | 5 mM | |
11 | DTT | natural abundance | 1 mM | |
12 | Protein | [U-13C; U-15N] | 0.3 mM |