NMR Structure of a tRNA 2'-phosphotransferase from Runella slithyformis
GSHMVKVSKF LSLVLRHNPA LIGLDLDANG WAPVKELLAK MKAKGHGISM EELKHIVETN SKKRFAFSEN FEKIRANQGH SVEVDLGYEK QVPPAVLFHG TAEKNFDLIL KDGIKKMSRH HVHLSQDITT ARKVGMRHGK PVVLSVDAKG MADGGFDFYL SNNGVWLIDF VPAEFIKV
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 87.7 % (1856 of 2117) | 85.6 % (939 of 1097) | 88.3 % (732 of 829) | 96.9 % (185 of 191) |
Backbone | 95.3 % (1006 of 1056) | 96.4 % (352 of 365) | 93.4 % (485 of 519) | 98.3 % (169 of 172) |
Sidechain | 82.2 % (1006 of 1224) | 80.2 % (587 of 732) | 85.2 % (403 of 473) | 84.2 % (16 of 19) |
Aromatic | 56.1 % (101 of 180) | 63.3 % (57 of 90) | 47.7 % (42 of 88) | 100.0 % (2 of 2) |
Methyl | 100.0 % (206 of 206) | 100.0 % (103 of 103) | 100.0 % (103 of 103) |
1. entity 1
GSHMVKVSKF LSLVLRHNPA LIGLDLDANG WAPVKELLAK MKAKGHGISM EELKHIVETN SKKRFAFSEN FEKIRANQGH SVEVDLGYEK QVPPAVLFHG TAEKNFDLIL KDGIKKMSRH HVHLSQDITT ARKVGMRHGK PVVLSVDAKG MADGGFDFYL SNNGVWLIDF VPAEFIKVSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 290.15 K, pH 7, Details 300 uM [U-13C; U-15N] Tpt1, 20 mM HEPES, 200 mM sodium chloride, 2 mM DTT, 2 mM [U-100% 2H] EDTA, 5 % v/v [U-100% 2H] glycerol, 0.5 mM AEBSF protease inhibitor, 0.04 % v/v sodium azide, 50 uM DSS, 95% H2O/5% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Tpt1 | [U-13C; U-15N] | 300 uM | |
2 | HEPES | natural abundance | 20 mM | |
3 | sodium chloride | natural abundance | 200 mM | |
4 | DTT | natural abundance | 2 mM | |
5 | EDTA | [U-100% 2H] | 2 mM | |
6 | glycerol | [U-100% 2H] | 5 % v/v | |
7 | AEBSF protease inhibitor | natural abundance | 0.5 mM | |
8 | sodium azide | natural abundance | 0.04 % v/v | |
9 | DSS | natural abundance | 50 uM |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 290.15 K, pH 7, Details 360 uM [U-13C; U-15N] Tpt1, 20 mM HEPES, 200 mM sodium chloride, 2 mM DTT, 2 mM [U-100% 2H] EDTA, 5 % v/v [U-100% 2H] glycerol, 0.5 mM AEBSF protease inhibitor, 0.04 % v/v sodium azide, 50 uM DSS, 95% H2O/5% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
10 | Tpt1 | [U-13C; U-15N] | 360 uM | |
11 | HEPES | natural abundance | 20 mM | |
12 | sodium chloride | natural abundance | 200 mM | |
13 | DTT | natural abundance | 2 mM | |
14 | EDTA | [U-100% 2H] | 2 mM | |
15 | glycerol | [U-100% 2H] | 5 % v/v | |
16 | AEBSF protease inhibitor | natural abundance | 0.5 mM | |
17 | sodium azide | natural abundance | 0.04 % v/v | |
18 | DSS | natural abundance | 50 uM |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 290.15 K, pH 7, Details 300 uM [U-13C; U-15N] Tpt1, 20 mM HEPES, 200 mM sodium chloride, 2 mM DTT, 2 mM [U-100% 2H] EDTA, 5 % v/v [U-100% 2H] glycerol, 1 mM AEBSF protease inhibitor, 0.05 % w/v sodium azide, 50 uM DSS, 95% H2O/5% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
19 | Tpt1 | [U-13C; U-15N] | 300 uM | |
20 | HEPES | natural abundance | 20 mM | |
21 | sodium chloride | natural abundance | 200 mM | |
22 | DTT | natural abundance | 2 mM | |
23 | EDTA | [U-100% 2H] | 2 mM | |
24 | glycerol | [U-100% 2H] | 5 % v/v | |
25 | AEBSF protease inhibitor | natural abundance | 1 mM | |
26 | sodium azide | natural abundance | 0.05 % w/v | |
27 | DSS | natural abundance | 50 uM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 290.15 K, pH 7, Details 400 uM [U-13C; U-15N] Tpt1, 20 mM HEPES, 200 mM sodium chloride, 2 mM DTT, 2 mM [U-100% 2H] EDTA, 5 % v/v [U-100% 2H] glycerol, 0.5 mM AEBSF protease inhibitor, 0.05 % w/v sodium azide, 50 mM DSS, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
28 | Tpt1 | [U-13C; U-15N] | 400 uM | |
29 | HEPES | natural abundance | 20 mM | |
30 | sodium chloride | natural abundance | 200 mM | |
31 | DTT | natural abundance | 2 mM | |
32 | EDTA | [U-100% 2H] | 2 mM | |
33 | glycerol | [U-100% 2H] | 5 % v/v | |
34 | AEBSF protease inhibitor | natural abundance | 0.5 mM | |
35 | sodium azide | natural abundance | 0.05 % w/v | |
36 | DSS | natural abundance | 50 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 290.15 K, pH 7, Details 400 uM [U-2H; U-15N];[13CD1,1H3]ILE;[13CD1,1H3;13CD2,1H3]LEU; [13CG1,1H3;13CG2,1H3]VAL;[13CE1,1H3]MET;[13CG2,1H3]THR Tpt1, 20 mM HEPES, 200 mM sodium chloride, 2 mM DTT, 2 mM [U-100% 2H] EDTA, 5 % v/v [U-100% 2H] glycerol, 0.5 mM AEBSF protease inhibitor, 0.05 % w/v sodium azide, 50 uM DSS, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
37 | Tpt1 | [U-2H; U-15N]; selective methyl groups | 400 uM | |
38 | HEPES | natural abundance | 20 mM | |
39 | sodium chloride | natural abundance | 200 mM | |
40 | DTT | natural abundance | 2 mM | |
41 | EDTA | [U-100% 2H] | 2 mM | |
42 | glycerol | [U-100% 2H] | 5 % v/v | |
43 | AEBSF protease inhibitor | natural abundance | 0.5 mM | |
44 | sodium azide | natural abundance | 0.05 % w/v | |
45 | DSS | natural abundance | 50 uM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 290.15 K, pH 7, Details 350 uM [U-2H; U-15N];[13CD1,1H3]ILE;[13CD1,1H3;13CD2,1H3]LEU; [13CG1,1H3;13CG2,1H3]VAL;[13CE1,1H3]MET;[13CG2,1H3]THR Tpt1, 20 mM HEPES, 200 mM sodium chloride, 2 mM DTT, 2 mM [U-100% 2H] EDTA, 5 % v/v [U-100% 2H] glycerol, 0.5 mM AEBSF protease inhibitor, 0.05 % w/v sodium azide, 50 uM DSS, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
46 | Tpt1 | [U-2H; U-15N]; selective methyl groups | 350 uM | |
47 | HEPES | natural abundance | 20 mM | |
48 | sodium chloride | natural abundance | 200 mM | |
49 | DTT | natural abundance | 2 mM | |
50 | EDTA | [U-100% 2H] | 2 mM | |
51 | glycerol | [U-100% 2H] | 5 % v/v | |
52 | AEBSF protease inhibitor | natural abundance | 0.5 mM | |
53 | sodium azide | natural abundance | 0.05 % w/v | |
54 | DSS | natural abundance | 50 uM |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 290.15 K, pH 7, Details 420 uM [U-13C; U-15N] Tpt1, 20 mM HEPES, 200 mM sodium chloride, 2 mM DTT, 2 mM [U-100% 2H] EDTA, 5 % v/v [U-100% 2H] glycerol, 0.5 mM AEBSF protease inhibitor, 0.04 % w/v sodium azide, 50 mM DSS, 95% H2O/5% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
55 | Tpt1 | [U-13C; U-15N] | 420 uM | |
56 | HEPES | natural abundance | 20 mM | |
57 | sodium chloride | natural abundance | 200 mM | |
58 | DTT | natural abundance | 2 mM | |
59 | EDTA | [U-100% 2H] | 2 mM | |
60 | glycerol | [U-100% 2H] | 5 % v/v | |
61 | AEBSF protease inhibitor | natural abundance | 0.5 mM | |
62 | sodium azide | natural abundance | 0.04 % w/v | |
63 | DSS | natural abundance | 50 mM |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 290.15 K, pH 7, Details 380 uM [U-13C; U-15N] Tpt1, 20 mM HEPES, 200 mM sodium chloride, 2 mM DTT, 2 mM [U-100% 2H] EDTA, 5 % v/v [U-100% 2H] glycerol, 0.5 mM AEBSF protease inhibitor, 0.04 % w/v sodium azide, 50 mM DSS, 95% H2O/5% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
64 | Tpt1 | [U-13C; U-15N] | 380 uM | |
65 | HEPES | natural abundance | 20 mM | |
66 | sodium chloride | natural abundance | 200 mM | |
67 | DTT | natural abundance | 2 mM | |
68 | EDTA | [U-100% 2H] | 2 mM | |
69 | glycerol | [U-100% 2H] | 5 % v/v | |
70 | AEBSF protease inhibitor | natural abundance | 0.5 mM | |
71 | sodium azide | natural abundance | 0.04 % w/v | |
72 | DSS | natural abundance | 50 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 290.15 K, pH 7, Details 400 uM [U-13C; U-15N] Tpt1, 20 mM HEPES, 200 mM sodium chloride, 2 mM DTT, 2 mM [U-100% 2H] EDTA, 5 % v/v [U-100% 2H] glycerol, 0.5 mM AEBSF protease inhibitor, 0.04 % w/v sodium azide, 50 mM DSS, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
73 | Tpt1 | [U-13C; U-15N] | 400 uM | |
74 | HEPES | natural abundance | 20 mM | |
75 | sodium chloride | natural abundance | 200 mM | |
76 | DTT | natural abundance | 2 mM | |
77 | EDTA | [U-100% 2H] | 2 mM | |
78 | glycerol | [U-100% 2H] | 5 % v/v | |
79 | AEBSF protease inhibitor | natural abundance | 0.5 mM | |
80 | sodium azide | natural abundance | 0.04 % w/v | |
81 | DSS | natural abundance | 50 mM |