BMRB: 30855

Structure of the HIV-1 gp41 transmembrane domain and cytoplasmic tail

Authors

Piai, A., Fu, Q., Sharp, A.K., Bighi, B., Brown, A.M., Chou, J.J.

Assembly

Transmembrane protein gp41

Entity

1. Transmembrane protein gp41 (polymer, Thiol state: not present), 180 monomers, 20817.93 × 3 Da Detail

NWLWYIRIFI IIVGSLIGLR IVFAVLSLVN RVRQGYSPLS FQTHLPTPRG PDRPEGIEEE GGERDRDRSI RLVNGSLALI WDDLRSLSLF SYHRLRDLLL IVTRIVELLG RRGWEALKYW WNLLQYWSQE LKNSAVSLLN ATAIAVGEGT DRVIEVVQGA SRAIRHIPRR IRQGLERILL

Total weight

62453.79 Da

Max. entity weight

20817.93 Da

Source organism

Human immunodeficiency virus 1

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.0 %, Completeness: 31.9 %, Completeness (bb): 59.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	31.9 % (706 of 2212)	18.9 % (215 of 1139)	39.6 % (339 of 857)	70.4 % (152 of 216)
Backbone	59.9 % (640 of 1068)	45.1 % (166 of 368)	61.6 % (324 of 526)	86.2 % (150 of 174)
Sidechain	6.3 % (82 of 1310)	6.4 % (49 of 771)	6.2 % (31 of 497)	4.8 % (2 of 42)
Aromatic	0.0 % (0 of 176)	0.0 % (0 of 88)	0.0 % (0 of 81)	0.0 % (0 of 7)
Methyl	8.3 % (22 of 264)	9.1 % (12 of 132)	7.6 % (10 of 132)

1. entity 1

NWLWYIRIFI IIVGSLIGLR IVFAVLSLVN RVRQGYSPLS FQTHLPTPRG PDRPEGIEEE GGERDRDRSI RLVNGSLALI WDDLRSLSLF SYHRLRDLLL IVTRIVELLG RRGWEALKYW WNLLQYWSQE LKNSAVSLLN ATAIAVGEGT DRVIEVVQGA SRAIRHIPRR IRQGLERILL

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.7, Details 1.2 mM [U-100% 13C; U-100% 15N; U-85% 2H] HIV1 gp41 TMD-CT, 40 mM DMPC, 80 mM DHPC, 40 mM MES, 1 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	HIV1 gp41 TMD-CT	[U-100% 13C; U-100% 15N; U-85% 2H]		1.2 mM
2	DMPC	natural abundance		40 mM
3	DHPC	natural abundance		80 mM
4	MES	natural abundance		40 mM
5	sodium azide	natural abundance		1 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.7, Details 1 mM [ILV-100% 13C; U-100% 15N; U-85% 2H] HIV1 gp41 TMD-CT, 40 mM DMPC, 80 mM DHPC, 40 mM MES, 1 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
6	HIV1 gp41 TMD-CT	[ILV-100% 13C; U-100% 15N; U-85% 2H]		1 mM
7	DMPC	natural abundance		40 mM
8	DHPC	natural abundance		80 mM
9	MES	natural abundance		40 mM
10	sodium azide	natural abundance		1 %

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Release date

2021-03-07

Citation

NMR Model of the Entire Membrane-Interacting Region of the HIV-1 Fusion Protein and Its Perturbation of Membrane Morphology
Piai, A., Fu, Q., Sharp, A.K., Bighi, B., Brown, A.M., Chou, J.J.
J. Am. Chem. Soc. (2021), 143, 6609-6615, PubMed 33882664 , DOI 10.1021/jacs.1c01762 , Abstract

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. Transmembrane protein gp41, : 1 : 1 : 77 entities Detail

Interaction partners 1. Transmembrane protein gp41, : 1 interactors Detail

Experiments performed 8 experiments Detail

Chemical shift validation 3 contents Detail