BMRBj - BMREntryMaster

Found 1 Document (0.806 seconds)

BMRB: 30857

7LP5

Structure of Nedd4L WW3 domain

Authors

Alam, S.L., Alian, A., Thompson, T., Rheinemann, L., Sundquist, W.I.

Assembly

E3 ubiquitin-protein ligase NEDD4-like (E.C.2.3.2.26)

Entity

1. E3 ubiquitin-protein ligase NEDD4-like (E.C.2.3.2.26) (polymer, Thiol state: not present), 47 monomers, 5596.402 Da Detail

KVTQSFLPPG WEMRIAPNGR PFFIDHNTKT TTWEDPRLKF PVHMRSK

Formula weight

5596.402 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 92.5 %, Completeness (bb): 95.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	92.5 % (553 of 598)	94.6 % (298 of 315)	89.7 % (209 of 233)	92.0 % (46 of 50)
Backbone	95.6 % (258 of 270)	97.8 % (88 of 90)	94.2 % (131 of 139)	95.1 % (39 of 41)
Sidechain	91.2 % (340 of 373)	93.3 % (210 of 225)	88.5 % (123 of 139)	77.8 % (7 of 9)
Aromatic	68.1 % (49 of 72)	77.8 % (28 of 36)	55.9 % (19 of 34)	100.0 % (2 of 2)
Methyl	100.0 % (36 of 36)	100.0 % (18 of 18)	100.0 % (18 of 18)

1. entity 1

KVTQSFLPPG WEMRIAPNGR PFFIDHNTKT TTWEDPRLKF PVHMRSK

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 7.5, Details [U-100% 13C; U-100% 15N] E3 ubiquitin-protein ligase NEDD4-like, 20 mM sodium phosphate, 50 mM sodium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	E3 ubiquitin-protein ligase NEDD4-like	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 7.5, Details [U-100% 15N] E3 ubiquitin-protein ligase NEDD4-like, 20 mM sodium phosphate, 50 mM sodium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
4	E3 ubiquitin-protein ligase NEDD4-like	[U-100% 15N]	0.0 ~ 0.0 mM
5	sodium phosphate	natural abundance	20 mM
6	sodium chloride	natural abundance	50 mM

Release date

2021-02-13

Citation

Interactions between AMOT PPxY motifs and NEDD4L WW domains function in HIV-1 release
Rheinemann, L., Thompson, T., Mercenne, G., Paine, E.L., Peterson, F.C., Volkman, B.F., Alam, S.L., Alian, A., Sundquist, W.I.
J. Biol. Chem. (2021), 297, 100975-100975, PubMed 34284061 , DOI 10.1016/j.jbc.2021.100975 , Abstract

Related entities 1. E3 ubiquitin-protein ligase NEDD4-like (E.C.2.3.2.26), : 1 : 7 : 1 : 82 entities Detail

Interaction partners 1. E3 ubiquitin-protein ligase NEDD4-like (E.C.2.3.2.26), : 10 interactors Detail

More details for 10 interactors identified by 4 citations

Experiments performed 19 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 7.5, Details [U-100% 15N] E3 ubiquitin-protein ligase NEDD4-like, 20 mM sodium phosphate, 50 mM sodium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
4	E3 ubiquitin-protein ligase NEDD4-like	[U-100% 15N]	0.0 ~ 0.0 mM
5	sodium phosphate	natural abundance	20 mM
6	sodium chloride	natural abundance	50 mM

2 2D 1H-15N HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 7.5, Details [U-100% 13C; U-100% 15N] E3 ubiquitin-protein ligase NEDD4-like, 20 mM sodium phosphate, 50 mM sodium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	E3 ubiquitin-protein ligase NEDD4-like	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM

3 2D 1H-13C HSQC aromatic

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	E3 ubiquitin-protein ligase NEDD4-like	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM

4 2D 1H-13C HSQC aliphatic

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	E3 ubiquitin-protein ligase NEDD4-like	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM

5 3D HNCACB

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	E3 ubiquitin-protein ligase NEDD4-like	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM

6 3D CBCA(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	E3 ubiquitin-protein ligase NEDD4-like	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM

7 3D H(CCO)NH

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	E3 ubiquitin-protein ligase NEDD4-like	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM

8 3D HCCH-TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	E3 ubiquitin-protein ligase NEDD4-like	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM

9 3D HNCO

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	E3 ubiquitin-protein ligase NEDD4-like	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM

10 3D HN(CO)CA

Instrument

Varian INOVA - 600 MHz

Sample

State anisotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 7.5, Details [U-100% 13C; U-100% 15N] E3 ubiquitin-protein ligase NEDD4-like, 20 mM sodium phosphate, 50 mM sodium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	E3 ubiquitin-protein ligase NEDD4-like	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM

11 3D HNCA

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	E3 ubiquitin-protein ligase NEDD4-like	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM

12 3D HNHA

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	E3 ubiquitin-protein ligase NEDD4-like	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM

13 3D HNHB

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	E3 ubiquitin-protein ligase NEDD4-like	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM

14 3D C(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	E3 ubiquitin-protein ligase NEDD4-like	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM

15 3D 1H-15N NOESY

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	E3 ubiquitin-protein ligase NEDD4-like	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM

16 3D 1H-15N NOESY

Instrument

Varian INOVA - 900 MHz

Sample

#	Name	Isotope labeling	Concentration
1	E3 ubiquitin-protein ligase NEDD4-like	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM

17 3D 1H-13C NOESY aliphatic

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	E3 ubiquitin-protein ligase NEDD4-like	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM

18 3D 1H-13C NOESY aliphatic

Instrument

Varian INOVA - 900 MHz

Sample

#	Name	Isotope labeling	Concentration
1	E3 ubiquitin-protein ligase NEDD4-like	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM

19 3D 1H-13C NOESY aromatic

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	E3 ubiquitin-protein ligase NEDD4-like	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr30857_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr30857_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	594		100.0 (chain: 1, length: 47)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 47, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 339
  - ¹³C chemical shifts: 209
  - ¹⁵N chemical shifts: 46
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
26	HIS	HD1	6.939

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	315	298	94.6
¹³C chemical shifts	233	209	89.7
¹⁵N chemical shifts	50	46	92.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	90	88	97.8
¹³C chemical shifts	94	86	91.5
¹⁵N chemical shifts	41	39	95.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	225	210	93.3
¹³C chemical shifts	139	123	88.5
¹⁵N chemical shifts	9	7	77.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	20	20	100.0
¹³C chemical shifts	20	20	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	36	28	77.8
¹³C chemical shifts	34	19	55.9
¹⁵N chemical shifts	2	2	100.0

Keywords E3 Ubiquitin ligase, LIGASE, Nedd4L, PPxY binding

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Found 1 Document (0.806 seconds)

BMRB: 30857

Sample #1

Sample #2

Related entities 1. E3 ubiquitin-protein ligase NEDD4-like (E.C.2.3.2.26), : 1 : 7 : 1 : 82 entities Detail

Interaction partners 1. E3 ubiquitin-protein ligase NEDD4-like (E.C.2.3.2.26), : 10 interactors Detail

Experiments performed 19 experiments Detail

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Chemical shift validation 3 contents Detail