NMR structure of the Human T-cell leukemia virus 1 matrix protein
MGRIFSRSAS PIPRPPRGLA AHHWLNFLQA AYRLEPGPSS YDFHQLKKFL KIALETPVWI CPINYSLLAS LLPKGYPGRV NEILHILIQT QAQIPSRPAH HHHHH
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 86.0 % (1110 of 1290) | 85.9 % (577 of 672) | 86.4 % (439 of 508) | 85.5 % (94 of 110) |
Backbone | 88.8 % (538 of 606) | 89.2 % (181 of 203) | 87.7 % (272 of 310) | 91.4 % (85 of 93) |
Sidechain | 84.7 % (664 of 784) | 84.4 % (396 of 469) | 86.9 % (259 of 298) | 52.9 % (9 of 17) |
Aromatic | 77.9 % (106 of 136) | 79.4 % (54 of 68) | 75.8 % (50 of 66) | 100.0 % (2 of 2) |
Methyl | 97.5 % (115 of 118) | 98.3 % (58 of 59) | 96.6 % (57 of 59) |
1. entity 1
MGRIFSRSAS PIPRPPRGLA AHHWLNFLQA AYRLEPGPSS YDFHQLKKFL KIALETPVWI CPINYSLLAS LLPKGYPGRV NEILHILIQT QAQIPSRPAH HHHHHSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 6.0, Details 100 mM sodium chloride, 50 mM sodium phosphate, 2 mM TCEP, 400 uM [U-99% 13C; U-99% 15N] HTLV-1 Matrix, 95% H2O/5% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | sodium chloride | natural abundance | 100 mM | |
2 | sodium phosphate | natural abundance | 50 mM | |
3 | TCEP | natural abundance | 2 mM | |
4 | HTLV-1 Matrix | [U-99% 13C; U-99% 15N] | 400 uM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.0, Details 100 mM sodium chloride, 50 mM sodium phosphate, 2 mM TCEP, 320 uM [U-99% 13C; U-99% 15N] HTLV-1 Matrix, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | sodium chloride | natural abundance | 100 mM | |
6 | sodium phosphate | natural abundance | 50 mM | |
7 | TCEP | natural abundance | 2 mM | |
8 | HTLV-1 Matrix | [U-99% 13C; U-99% 15N] | 320 uM |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 6.0, Details 100 mM sodium chloride, 50 mM sodium phosphate, 2 mM TCEP, 500 uM [U-99% 15N] HTLV-1 Matrix, 95% H2O/5% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
9 | sodium chloride | natural abundance | 100 mM | |
10 | sodium phosphate | natural abundance | 50 mM | |
11 | TCEP | natural abundance | 2 mM | |
12 | HTLV-1 Matrix | [U-99% 15N] | 500 uM |