Atomic-Resolution Structure of Kinesin-1 Motor Domain in Complex with Polymeric Microtubules by Magic Angle Spinning NMR
MADLAECNIK VMCRFRPLNE SEVNRGDKYI AKFQGEDTVV IASKPYAFDR VFQSSTSQEQ VYNDCAKKIV KDVLEGYNGT IFAYGQTSSG KTHTMEGKLH DPEGMGIIPR IVQDIFNYIY SMDENLEFHI KVSYFEIYLD KIRDLLDVSK TNLSVHEDKN RVPYVKGCTE RFVCSPDEVM DTIDEGKSNR HVAVTNMNEH SSRSHSIFLI NVKQENTQTE QKLSGKLYLV DLAGSEKVSK TGAEGAVLDE AKNINKSLSA LGNVISALAE GSTYVPYRDS KMTRILQDSL GGNCRTTIVI CCSPSSYNES ETKSTLLFGQ RAKTIKNTVC VNVELTAEQW KKKYEKEKE
Polymer type: polypeptide(L)
Total | 13C | 15N | |
---|---|---|---|
All | 68.0 % (1330 of 1957) | 67.1 % (1052 of 1568) | 71.5 % (278 of 389) |
Backbone | 79.8 % (1091 of 1368) | 79.3 % (814 of 1027) | 81.2 % (277 of 341) |
Sidechain | 54.7 % (502 of 918) | 57.6 % (501 of 870) | 2.1 % (1 of 48) |
Aromatic | 33.3 % (45 of 135) | 32.8 % (44 of 134) | 100.0 % (1 of 1) |
Methyl | 64.9 % (120 of 185) | 64.9 % (120 of 185) |
1. entity 1
MADLAECNIK VMCRFRPLNE SEVNRGDKYI AKFQGEDTVV IASKPYAFDR VFQSSTSQEQ VYNDCAKKIV KDVLEGYNGT IFAYGQTSSG KTHTMEGKLH DPEGMGIIPR IVQDIFNYIY SMDENLEFHI KVSYFEIYLD KIRDLLDVSK TNLSVHEDKN RVPYVKGCTE RFVCSPDEVM DTIDEGKSNR HVAVTNMNEH SSRSHSIFLI NVKQENTQTE QKLSGKLYLV DLAGSEKVSK TGAEGAVLDE AKNINKSLSA LGNVISALAE GSTYVPYRDS KMTRILQDSL GGNCRTTIVI CCSPSSYNES ETKSTLLFGQ RAKTIKNTVC VNVELTAEQW KKKYEKEKESolvent system 100% H2O, Pressure 1 atm, Temperature 283 K, pH 7.4, Details 9.1 % w/w [U-100% 13C; U-100% 15N] Apo-Kif5b, 16.4 % w/w Porcine-alpha-tubulin, 16.4 % w/w Porcine-beta-tubulin, 5 uM TAXOL, 1 mM GUANOSINE-5'-TRIPHOSPHATE, 1 mM GUANOSINE-5'-DIPHOSPHATE, 58.1 % w/w Buffer, 100% H2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Apo-Kif5b | [U-100% 13C; U-100% 15N] | 9.1 % w/w | |
2 | Porcine-alpha-tubulin | natural abundance | 16.4 % w/w | |
3 | Porcine-beta-tubulin | natural abundance | 16.4 % w/w | |
4 | TAXOL | natural abundance | 5 uM | |
5 | GUANOSINE-5'-TRIPHOSPHATE | natural abundance | 1 mM | |
6 | GUANOSINE-5'-DIPHOSPHATE | natural abundance | 1 mM | |
7 | Buffer | natural abundance | 58.1 % w/w |
Solvent system 100% H2O, Pressure 1 atm, Temperature 283 K, pH 7.4, Details 9.1 % w/w [U-100% 13C; U-100% 15N] Apo-Kif5b, 16.4 % w/w Porcine-alpha-tubulin, 16.4 % w/w Porcine-beta-tubulin, 5 uM TAXOL, 1 mM GUANOSINE-5'-TRIPHOSPHATE, 1 mM GUANOSINE-5'-DIPHOSPHATE, 58.1 % w/w Buffer, 100% H2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
8 | Apo-Kif5b | [U-100% 13C; U-100% 15N] | 9.1 % w/w | |
9 | Porcine-alpha-tubulin | natural abundance | 16.4 % w/w | |
10 | Porcine-beta-tubulin | natural abundance | 16.4 % w/w | |
11 | TAXOL | natural abundance | 5 uM | |
12 | GUANOSINE-5'-TRIPHOSPHATE | natural abundance | 1 mM | |
13 | GUANOSINE-5'-DIPHOSPHATE | natural abundance | 1 mM | |
14 | Buffer | natural abundance | 58.1 % w/w |
Solvent system 100% H2O, Pressure 1 atm, Temperature 283 K, pH 7.4, Details 8.2 % w/w [1,6-13C-glucose; U-100% 15N] Apo-Kif5b, 16.4 % w/w Porcine-alpha-tubulin, 16.4 % w/w Porcine-beta-tubulin, 5 uM TAXOL, 1 mM GUANOSINE-5'-TRIPHOSPHATE, 1 mM GUANOSINE-5'-DIPHOSPHATE, 59 % w/w Buffer, 100% H2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
15 | Apo-Kif5b | [1,6-13C-glucose; U-100% 15N] | 8.2 % w/w | |
16 | Porcine-alpha-tubulin | natural abundance | 16.4 % w/w | |
17 | Porcine-beta-tubulin | natural abundance | 16.4 % w/w | |
18 | TAXOL | natural abundance | 5 uM | |
19 | GUANOSINE-5'-TRIPHOSPHATE | natural abundance | 1 mM | |
20 | GUANOSINE-5'-DIPHOSPHATE | natural abundance | 1 mM | |
21 | Buffer | natural abundance | 59 % w/w |
Solvent system 100% H2O, Pressure 1 atm, Temperature 283 K, pH 7.4, Details 7.6 % w/w [2-13C-glucose; U-100% 15N] Apo-Kif5b, 16.4 % w/w Porcine-alpha-tubulin, 16.4 % w/w Porcine-beta-tubulin, 5 uM TAXOL, 1 mM GUANOSINE-5'-TRIPHOSPHATE, 1 mM GUANOSINE-5'-DIPHOSPHATE, 59.6 % w/w Buffer, 100% H2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
22 | Apo-Kif5b | [2-13C-glucose; U-100% 15N] | 7.6 % w/w | |
23 | Porcine-alpha-tubulin | natural abundance | 16.4 % w/w | |
24 | Porcine-beta-tubulin | natural abundance | 16.4 % w/w | |
25 | TAXOL | natural abundance | 5 uM | |
26 | GUANOSINE-5'-TRIPHOSPHATE | natural abundance | 1 mM | |
27 | GUANOSINE-5'-DIPHOSPHATE | natural abundance | 1 mM | |
28 | Buffer | natural abundance | 59.6 % w/w |