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BMRB: 30939

8F4V

Structures of the Intracellular Domain and Transmembrane Domain of the Human alpha7 Nicotinic Acetylcholine Receptors

Authors

Bondarenko, V., Chen, Q., Tang, P.

Assembly

Neuronal acetylcholine receptor subunit alpha-7

Entity

1. Neuronal acetylcholine receptor subunit alpha-7 (polymer), 272 monomers, 29803.50 × 5 Da Detail

SNAEEELYYG LNLLIPCVLI SALALLVFLL PADSGEKISL GITVLLSLTV FMLLVAEIMP STSDSSPSIA QYFASTMIIV GLSVVVTVIV LQYHHHDPDG GKMPKWTRVI LLNWCAWFLR MKRPGEDKVR PACQHKQRRC SLASVEMSAV APPPASNGNL LYIGFRGLDG VHCVPTPDSG VVCGRMACSP THDEHLLHGG QPPEGDPDLA KILEEVRYIA NRFRCQDESE AVCSEWKFAA CVVDRLCLMA FSVFTIICTI GILMSAPNFV EE

Total weight

149017.5 Da

Max. entity weight

29803.5 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.7 %, Completeness: 62.3 %, Completeness (bb): 84.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	62.3 % (1943 of 3118)	56.4 % (900 of 1595)	64.4 % (798 of 1240)	86.6 % (245 of 283)
Backbone	84.8 % (1353 of 1596)	85.3 % (463 of 543)	81.9 % (654 of 799)	92.9 % (236 of 254)
Sidechain	43.8 % (778 of 1777)	41.5 % (437 of 1052)	47.7 % (332 of 696)	31.0 % (9 of 29)
Aromatic	21.1 % (48 of 228)	23.7 % (27 of 114)	15.5 % (17 of 110)	100.0 % (4 of 4)
Methyl	62.3 % (223 of 358)	67.6 % (121 of 179)	57.0 % (102 of 179)

1. entity 1

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.24 mM [U-13C; U-15N] alpha7nAChR TMD+ICD, 5 mM sodium acetate, 66 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	alpha7nAChR TMD+ICD	[U-13C; U-15N]	0.24 (±0.01) mM
2	sodium acetate	natural abundance	5 mM
3	LDAO	natural abundance	66 mM
4	NaCl	natural abundance	25 mM
5	D2O	natural abundance	5 %
6	DSS	natural abundance	20 uM

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.25 mM [U-13C; U-15N] alpha7nAChR C317 TMD+ICD, 5 mM sodium acetate, 44 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
7	alpha7nAChR C317 TMD+ICD	[U-13C; U-15N]	0.25 (±0.01) mM
8	sodium acetate	natural abundance	5 mM
9	LDAO	natural abundance	44 mM
10	NaCl	natural abundance	25 mM
11	D2O	natural abundance	5 %
12	DSS	natural abundance	20 uM

Sample #3

#	Name	Isotope labeling	Concentration
13	alpha7nAChR C317 TMD+ICD	[U-13C; U-15N]	0.25 (±0.01) mM
14	sodium acetate	natural abundance	5 mM
15	LDAO	natural abundance	44 mM
16	NaCl	natural abundance	25 mM
17	D2O	natural abundance	5 %
18	DSS	natural abundance	20 uM

Sample #4

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.35 mM [U-15N] alpha7nAChR C317 TMD+ICD, 5 mM sodium acetate, 66 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
19	alpha7nAChR C317 TMD+ICD	[U-15N]	0.35 (±0.01) mM
20	sodium acetate	natural abundance	5 mM
21	LDAO	natural abundance	66 mM
22	NaCl	natural abundance	25 mM
23	D2O	natural abundance	5 %
24	DSS	natural abundance	20 uM

Sample #5

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.22 mM [U-15N] V311C-MTSL alpha7nAChR TMD+ICD, 5 mM sodium acetate, 44 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
25	V311C-MTSL alpha7nAChR TMD+ICD	[U-15N]	0.22 (±0.01) mM
26	sodium acetate	natural abundance	5 mM
27	LDAO	natural abundance	44 mM
28	NaCl	natural abundance	25 mM
29	D2O	natural abundance	5 %
30	DSS	natural abundance	20 uM

Sample #6

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.22 mM [U-15N] C317-MTSL alpha7nAChR TMD+ICD, 5 mM sodium acetate, 44 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
31	C317-MTSL alpha7nAChR TMD+ICD	[U-15N]	0.22 (±0.01) mM
32	sodium acetate	natural abundance	5 mM
33	LDAO	natural abundance	44 mM
34	NaCl	natural abundance	25 mM
35	D2O	natural abundance	5 %
36	DSS	natural abundance	20 uM

Sample #7

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.3 mM [U-15N] C335-MTSL alpha7nAChR TMD+ICD, 5 mM sodium acetate, 53 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
37	C335-MTSL alpha7nAChR TMD+ICD	[U-15N]	0.3 (±0.01) mM
38	sodium acetate	natural abundance	5 mM
39	LDAO	natural abundance	53 mM
40	NaCl	natural abundance	25 mM
41	D2O	natural abundance	5 %
42	DSS	natural abundance	20 uM

Sample #8

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.3 mM [U-15N] C342-MTSL alpha7nAChR TMD+ICD, 5 mM sodium acetate, 66 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
43	C342-MTSL alpha7nAChR TMD+ICD	[U-15N]	0.3 (±0.01) mM
44	sodium acetate	natural abundance	5 mM
45	LDAO	natural abundance	66 mM
46	NaCl	natural abundance	25 mM
47	D2O	natural abundance	5 %
48	DSS	natural abundance	20 uM

Sample #9

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.3 mM [U-15N] S350C-MTSL alpha7nAChR TMD+ICD, 5 mM sodium acetate, 53 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
49	S350C-MTSL alpha7nAChR TMD+ICD	[U-15N]	0.3 (±0.01) mM
50	sodium acetate	natural abundance	5 mM
51	LDAO	natural abundance	53 mM
52	NaCl	natural abundance	25 mM
53	D2O	natural abundance	5 %
54	DSS	natural abundance	20 uM

Sample #10

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.25 mM [U-15N] S358C-MTSL alpha7nAChR TMD+ICD, 5 mM sodium acetate, 44 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
55	S358C-MTSL alpha7nAChR TMD+ICD	[U-15N]	0.25 (±0.01) mM
56	sodium acetate	natural abundance	5 mM
57	LDAO	natural abundance	44 mM
58	NaCl	natural abundance	25 mM
59	D2O	natural abundance	5 %
60	DSS	natural abundance	20 uM

Sample #11

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.25 mM [U-15N] C375-MTSL alpha7nAChR TMD+ICD, 5 mM sodium acetate, 66 mM LDAO, 20 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
61	C375-MTSL alpha7nAChR TMD+ICD	[U-15N]	0.25 (±0.01) mM
62	sodium acetate	natural abundance	5 mM
63	LDAO	natural abundance	66 mM
64	NaCl	natural abundance	20 mM
65	D2O	natural abundance	5 %
66	DSS	natural abundance	20 uM

Sample #12

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.22 mM [U-15N] C390-MTSL alpha7nAChR TMD+ICD, 5 mM sodium acetate, 53 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
67	C390-MTSL alpha7nAChR TMD+ICD	[U-15N]	0.22 (±0.01) mM
68	sodium acetate	natural abundance	5 mM
69	LDAO	natural abundance	53 mM
70	NaCl	natural abundance	25 mM
71	D2O	natural abundance	5 %
72	DSS	natural abundance	20 uM

Sample #13

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.25 mM [U-15N] L415C-MTSL alpha7nAChR TMD+ICD, 5 mM sodium acetate, 44 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
73	L415C-MTSL alpha7nAChR TMD+ICD	[U-15N]	0.25 (±0.01) mM
74	sodium acetate	natural abundance	5 mM
75	LDAO	natural abundance	44 mM
76	NaCl	natural abundance	25 mM
77	D2O	natural abundance	5 %
78	DSS	natural abundance	20 uM

Sample #14

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.25 mM [U-15N] C427-MTSL alpha7nAChR TMD+ICD, 5 mM sodium acetate, 44 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
79	C427-MTSL alpha7nAChR TMD+ICD	[U-15N]	0.25 (±0.01) mM
80	sodium acetate	natural abundance	5 mM
81	LDAO	natural abundance	44 mM
82	NaCl	natural abundance	25 mM
83	D2O	natural abundance	5 %
84	DSS	natural abundance	20 uM

Sample #15

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.25 mM [U-15N] C435-MTSL alpha7nAChR TMD+ICD, 5 mM sodium acetate, 44 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
85	C435-MTSL alpha7nAChR TMD+ICD	[U-15N]	0.25 (±0.01) mM
86	sodium acetate	natural abundance	5 mM
87	LDAO	natural abundance	44 mM
88	NaCl	natural abundance	25 mM
89	D2O	natural abundance	5 %
90	DSS	natural abundance	20 uM

Sample #16

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.28 mM V311C-MTSL alpha7 TMD-ICD, 0.07 mM [U-15N] V311C alpha7 TMD-ICD, 5 mM sodium acetate, 44 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
91	V311C-MTSL alpha7 TMD-ICD	natural abundance	0.28 (±0.01) mM
92	V311C alpha7 TMD-ICD	[U-15N]	0.07 (±0.005) mM
93	sodium acetate	natural abundance	5 mM
94	LDAO	natural abundance	44 mM
95	NaCl	natural abundance	25 mM
96	D2O	natural abundance	5 %
97	DSS	natural abundance	20 uM

Sample #17

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.24 mM C427-MTSL alpha7 TMD-ICD, 0.06 mM [U-15N] C427 alpha7 TMD-ICD, 5 mM sodium acetate, 44 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
98	C427-MTSL alpha7 TMD-ICD	natural abundance	0.24 (±0.01) mM
99	C427 alpha7 TMD-ICD	[U-15N]	0.06 (±0.005) mM
100	sodium acetate	natural abundance	5 mM
101	LDAO	natural abundance	44 mM
102	NaCl	natural abundance	25 mM
103	D2O	natural abundance	5 %
104	DSS	natural abundance	20 uM

Sample #18

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.28 mM V311C alpha7 TMD-ICD, 0.07 mM [U-15N] V311C-MTSL alpha7 TMD-ICD, 5 mM sodium acetate, 44 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
105	V311C-MTSL alpha7 TMD-ICD	[U-15N]	0.07 (±0.005) mM
106	V311C alpha7 TMD-ICD	natural abundance	0.28 (±0.01) mM
107	sodium acetate	natural abundance	5 mM
108	LDAO	natural abundance	44 mM
109	NaCl	natural abundance	25 mM
110	D2O	natural abundance	5 %
111	DSS	natural abundance	20 uM

Sample #19

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.24 mM C427 alpha7 TMD-ICD, 0.06 mM [U-15N] C427-MTSL alpha7 TMD-ICD, 5 mM sodium acetate, 44 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
112	C427-MTSL alpha7 TMD-ICD	[U-15N]	0.06 (±0.005) mM
113	C427 alpha7 TMD-ICD	natural abundance	0.24 (±0.01) mM
114	sodium acetate	natural abundance	5 mM
115	LDAO	natural abundance	44 mM
116	NaCl	natural abundance	25 mM
117	D2O	natural abundance	5 %
118	DSS	natural abundance	20 uM

Sample #20

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.25 mM [U-15N] C427-EDTA-Lu3+ alpha7 TMD-ICD, 5 mM sodium acetate, 53 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
119	C427-EDTA-Lu3+ alpha7 TMD-ICD	[U-15N]	0.25 (±0.01) mM
120	sodium acetate	natural abundance	5 mM
121	LDAO	natural abundance	53 mM
122	NaCl	natural abundance	25 mM
123	D2O	natural abundance	5 %
124	DSS	natural abundance	20 uM

Sample #21

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.2 mM [U-15N] C427-EDTA-Tm3+ alpha7 TMD-ICD, 5 mM sodium acetate, 66 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
125	C427-EDTA-Tm3+ alpha7 TMD-ICD	[U-15N]	0.2 (±0.01) mM
126	sodium acetate	natural abundance	5 mM
127	LDAO	natural abundance	66 mM
128	NaCl	natural abundance	25 mM
129	D2O	natural abundance	5 %
130	DSS	natural abundance	20 uM

Sample #22

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.25 mM [U-15N] C317-EDTA-Lu3+ alpha7 TMD-ICD, 5 mM sodium acetate, 66 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
131	C317-EDTA-Lu3+ alpha7 TMD-ICD	[U-15N]	0.25 (±0.01) mM
132	sodium acetate	natural abundance	5 mM
133	LDAO	natural abundance	66 mM
134	NaCl	natural abundance	25 mM
135	D2O	natural abundance	5 %
136	DSS	natural abundance	20 uM

Sample #23

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.25 mM [U-15N] C317-EDTA-Tm3+ alpha7 TMD-ICD, 5 mM sodium acetate, 53 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
137	C317-EDTA-Tm3+ alpha7 TMD-ICD	[U-15N]	0.25 (±0.01) mM
138	sodium acetate	natural abundance	5 mM
139	LDAO	natural abundance	53 mM
140	NaCl	natural abundance	25 mM
141	D2O	natural abundance	5 %
142	DSS	natural abundance	20 uM

Sample #24

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.24 mM [U-15N] C219-EDTA-Lu3+ alpha7 TMD-ICD, 5 mM sodium acetate, 44 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
143	C219-EDTA-Lu3+ alpha7 TMD-ICD	[U-15N]	0.24 (±0.01) mM
144	sodium acetate	natural abundance	5 mM
145	LDAO	natural abundance	44 mM
146	NaCl	natural abundance	25 mM
147	D2O	natural abundance	5 %
148	DSS	natural abundance	20 uM

Sample #25

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.22 mM [U-15N] C219-EDTA-Tm3+ alpha7 TMD-ICD, 5 mM sodium acetate, 53 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
149	C219-EDTA-Tm3+ alpha7 TMD-ICD	[U-15N]	0.22 (±0.01) mM
150	sodium acetate	natural abundance	5 mM
151	LDAO	natural abundance	53 mM
152	NaCl	natural abundance	25 mM
153	D2O	natural abundance	5 %
154	DSS	natural abundance	20 uM

Sample #26

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.35 mM [U-13C; U-15N] alpha7nAChR C317 TMD+ICD, 5 mM sodium acetate, 44 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
155	alpha7nAChR C317 TMD+ICD	[U-13C; U-15N]	0.35 (±0.01) mM
156	sodium acetate	natural abundance	5 mM
157	LDAO	natural abundance	44 mM
158	NaCl	natural abundance	25 mM
159	D2O	natural abundance	5 %
160	DSS	natural abundance	20 uM

Release date

2021-08-07

Citation

Structures of highly flexible intracellular domain of human α7 nicotinic acetylcholine receptor
Bondarenko, V., Wells, M.M., Chen, Q., Tillman, T.S., Singewald, K., Lawless, M.J., Caporoso, J., Brandon, N., Coleman, J.A., Saxena, S., Lindahl, E., Xu, Y., Tang, P.
Nat. Commun. (2022), 13, 793-793, PubMed 35145092 , DOI 10.1038/s41467-022-28400-x , Abstract

Related entities 1. Neuronal acetylcholine receptor subunit alpha-7, : 1 : 1 : 233 entities Detail

Interaction partners 1. Neuronal acetylcholine receptor subunit alpha-7, : 6 interactors Detail

More details for 6 interactors identified by 4 citations

Experiments performed 48 experiments Detail

1 2D 1H-15N TROSY-HSQC

Instrument

Bruker AVANCE II - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.24 mM [U-13C; U-15N] alpha7nAChR TMD+ICD, 5 mM sodium acetate, 66 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	alpha7nAChR TMD+ICD	[U-13C; U-15N]	0.24 (±0.01) mM
2	sodium acetate	natural abundance	5 mM
3	LDAO	natural abundance	66 mM
4	NaCl	natural abundance	25 mM
5	D2O	natural abundance	5 %
6	DSS	natural abundance	20 uM

2 3D BEST-HNCA

Instrument

Bruker AVANCE II - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	alpha7nAChR TMD+ICD	[U-13C; U-15N]	0.24 (±0.01) mM
2	sodium acetate	natural abundance	5 mM
3	LDAO	natural abundance	66 mM
4	NaCl	natural abundance	25 mM
5	D2O	natural abundance	5 %
6	DSS	natural abundance	20 uM

3 3D BEST-HN(CO)CA

Instrument

Bruker AVANCE II - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	alpha7nAChR TMD+ICD	[U-13C; U-15N]	0.24 (±0.01) mM
2	sodium acetate	natural abundance	5 mM
3	LDAO	natural abundance	66 mM
4	NaCl	natural abundance	25 mM
5	D2O	natural abundance	5 %
6	DSS	natural abundance	20 uM

4 3D TROSY-CBCACONH

Instrument

Bruker AVANCE II - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	alpha7nAChR TMD+ICD	[U-13C; U-15N]	0.24 (±0.01) mM
2	sodium acetate	natural abundance	5 mM
3	LDAO	natural abundance	66 mM
4	NaCl	natural abundance	25 mM
5	D2O	natural abundance	5 %
6	DSS	natural abundance	20 uM

5 3D BEST-HNCO

Instrument

Bruker AVANCE II - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	alpha7nAChR TMD+ICD	[U-13C; U-15N]	0.24 (±0.01) mM
2	sodium acetate	natural abundance	5 mM
3	LDAO	natural abundance	66 mM
4	NaCl	natural abundance	25 mM
5	D2O	natural abundance	5 %
6	DSS	natural abundance	20 uM

6 2D 1H-15N TROSY-HSQC

Instrument

Bruker AVANCE II - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.25 mM [U-13C; U-15N] alpha7nAChR C317 TMD+ICD, 5 mM sodium acetate, 44 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
7	alpha7nAChR C317 TMD+ICD	[U-13C; U-15N]	0.25 (±0.01) mM
8	sodium acetate	natural abundance	5 mM
9	LDAO	natural abundance	44 mM
10	NaCl	natural abundance	25 mM
11	D2O	natural abundance	5 %
12	DSS	natural abundance	20 uM

7 3D TROSY-HNCA

Instrument

Bruker AVANCE II - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
7	alpha7nAChR C317 TMD+ICD	[U-13C; U-15N]	0.25 (±0.01) mM
8	sodium acetate	natural abundance	5 mM
9	LDAO	natural abundance	44 mM
10	NaCl	natural abundance	25 mM
11	D2O	natural abundance	5 %
12	DSS	natural abundance	20 uM

8 3D TROSY-HN(CO)CA

Instrument

Bruker AVANCE II - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
7	alpha7nAChR C317 TMD+ICD	[U-13C; U-15N]	0.25 (±0.01) mM
8	sodium acetate	natural abundance	5 mM
9	LDAO	natural abundance	44 mM
10	NaCl	natural abundance	25 mM
11	D2O	natural abundance	5 %
12	DSS	natural abundance	20 uM

9 3D TROSY-HNCACB

Instrument

Bruker AVANCE - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
7	alpha7nAChR C317 TMD+ICD	[U-13C; U-15N]	0.25 (±0.01) mM
8	sodium acetate	natural abundance	5 mM
9	LDAO	natural abundance	44 mM
10	NaCl	natural abundance	25 mM
11	D2O	natural abundance	5 %
12	DSS	natural abundance	20 uM

10 3D TROSY-CBCACONH

Instrument

Bruker AVANCE - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
7	alpha7nAChR C317 TMD+ICD	[U-13C; U-15N]	0.25 (±0.01) mM
8	sodium acetate	natural abundance	5 mM
9	LDAO	natural abundance	44 mM
10	NaCl	natural abundance	25 mM
11	D2O	natural abundance	5 %
12	DSS	natural abundance	20 uM

11 3D TROSY-HNCO

Instrument

Bruker AVANCE - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
7	alpha7nAChR C317 TMD+ICD	[U-13C; U-15N]	0.25 (±0.01) mM
8	sodium acetate	natural abundance	5 mM
9	LDAO	natural abundance	44 mM
10	NaCl	natural abundance	25 mM
11	D2O	natural abundance	5 %
12	DSS	natural abundance	20 uM

12 2D 1H-13C HSQC

Instrument

Bruker AVANCE - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
13	alpha7nAChR C317 TMD+ICD	[U-13C; U-15N]	0.25 (±0.01) mM
14	sodium acetate	natural abundance	5 mM
15	LDAO	natural abundance	44 mM
16	NaCl	natural abundance	25 mM
17	D2O	natural abundance	5 %
18	DSS	natural abundance	20 uM

13 3D 13C-edited NOESY-HSQC

Instrument

Bruker AVANCE - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
13	alpha7nAChR C317 TMD+ICD	[U-13C; U-15N]	0.25 (±0.01) mM
14	sodium acetate	natural abundance	5 mM
15	LDAO	natural abundance	44 mM
16	NaCl	natural abundance	25 mM
17	D2O	natural abundance	5 %
18	DSS	natural abundance	20 uM

14 2D 1H-15N TROSY-HSQC

Instrument

Bruker AVANCE II - 900 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.35 mM [U-15N] alpha7nAChR C317 TMD+ICD, 5 mM sodium acetate, 66 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
19	alpha7nAChR C317 TMD+ICD	[U-15N]	0.35 (±0.01) mM
20	sodium acetate	natural abundance	5 mM
21	LDAO	natural abundance	66 mM
22	NaCl	natural abundance	25 mM
23	D2O	natural abundance	5 %
24	DSS	natural abundance	20 uM

15 3D 15N-edited NOESY-TROSY-HSQC

Instrument

Bruker AVANCE II - 900 MHz

Sample

#	Name	Isotope labeling	Concentration
19	alpha7nAChR C317 TMD+ICD	[U-15N]	0.35 (±0.01) mM
20	sodium acetate	natural abundance	5 mM
21	LDAO	natural abundance	66 mM
22	NaCl	natural abundance	25 mM
23	D2O	natural abundance	5 %
24	DSS	natural abundance	20 uM

16 2D 1H-15N TROSY-HSQC

Instrument

Bruker AVANCE II - 900 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.22 mM [U-15N] V311C-MTSL alpha7nAChR TMD+ICD, 5 mM sodium acetate, 44 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
25	V311C-MTSL alpha7nAChR TMD+ICD	[U-15N]	0.22 (±0.01) mM
26	sodium acetate	natural abundance	5 mM
27	LDAO	natural abundance	44 mM
28	NaCl	natural abundance	25 mM
29	D2O	natural abundance	5 %
30	DSS	natural abundance	20 uM

17 2D 1H-15N TROSY-HSQC

Instrument

Bruker AVANCE II - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.22 mM [U-15N] C317-MTSL alpha7nAChR TMD+ICD, 5 mM sodium acetate, 44 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
31	C317-MTSL alpha7nAChR TMD+ICD	[U-15N]	0.22 (±0.01) mM
32	sodium acetate	natural abundance	5 mM
33	LDAO	natural abundance	44 mM
34	NaCl	natural abundance	25 mM
35	D2O	natural abundance	5 %
36	DSS	natural abundance	20 uM

18 2D 1H-15N TROSY-HSQC

Instrument

Bruker AVANCE II - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.3 mM [U-15N] C335-MTSL alpha7nAChR TMD+ICD, 5 mM sodium acetate, 53 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
37	C335-MTSL alpha7nAChR TMD+ICD	[U-15N]	0.3 (±0.01) mM
38	sodium acetate	natural abundance	5 mM
39	LDAO	natural abundance	53 mM
40	NaCl	natural abundance	25 mM
41	D2O	natural abundance	5 %
42	DSS	natural abundance	20 uM

19 2D 1H-15N TROSY-HSQC

Instrument

Bruker AVANCE II - 900 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.3 mM [U-15N] C342-MTSL alpha7nAChR TMD+ICD, 5 mM sodium acetate, 66 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
43	C342-MTSL alpha7nAChR TMD+ICD	[U-15N]	0.3 (±0.01) mM
44	sodium acetate	natural abundance	5 mM
45	LDAO	natural abundance	66 mM
46	NaCl	natural abundance	25 mM
47	D2O	natural abundance	5 %
48	DSS	natural abundance	20 uM

20 2D 1H-15N TROSY-HSQC

Instrument

Bruker AVANCE II - 900 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.3 mM [U-15N] S350C-MTSL alpha7nAChR TMD+ICD, 5 mM sodium acetate, 53 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
49	S350C-MTSL alpha7nAChR TMD+ICD	[U-15N]	0.3 (±0.01) mM
50	sodium acetate	natural abundance	5 mM
51	LDAO	natural abundance	53 mM
52	NaCl	natural abundance	25 mM
53	D2O	natural abundance	5 %
54	DSS	natural abundance	20 uM

21 2D 1H-15N TROSY-HSQC

Instrument

Bruker AVANCE II - 900 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.25 mM [U-15N] S358C-MTSL alpha7nAChR TMD+ICD, 5 mM sodium acetate, 44 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
55	S358C-MTSL alpha7nAChR TMD+ICD	[U-15N]	0.25 (±0.01) mM
56	sodium acetate	natural abundance	5 mM
57	LDAO	natural abundance	44 mM
58	NaCl	natural abundance	25 mM
59	D2O	natural abundance	5 %
60	DSS	natural abundance	20 uM

22 2D 1H-15N TROSY-HSQC

Instrument

Bruker AVANCE II - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.25 mM [U-15N] C375-MTSL alpha7nAChR TMD+ICD, 5 mM sodium acetate, 66 mM LDAO, 20 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
61	C375-MTSL alpha7nAChR TMD+ICD	[U-15N]	0.25 (±0.01) mM
62	sodium acetate	natural abundance	5 mM
63	LDAO	natural abundance	66 mM
64	NaCl	natural abundance	20 mM
65	D2O	natural abundance	5 %
66	DSS	natural abundance	20 uM

23 2D 1H-15N TROSY-HSQC

Instrument

Bruker AVANCE II - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.22 mM [U-15N] C390-MTSL alpha7nAChR TMD+ICD, 5 mM sodium acetate, 53 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
67	C390-MTSL alpha7nAChR TMD+ICD	[U-15N]	0.22 (±0.01) mM
68	sodium acetate	natural abundance	5 mM
69	LDAO	natural abundance	53 mM
70	NaCl	natural abundance	25 mM
71	D2O	natural abundance	5 %
72	DSS	natural abundance	20 uM

24 2D 1H-15N TROSY-HSQC

Instrument

Bruker AVANCE II - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.25 mM [U-15N] L415C-MTSL alpha7nAChR TMD+ICD, 5 mM sodium acetate, 44 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
73	L415C-MTSL alpha7nAChR TMD+ICD	[U-15N]	0.25 (±0.01) mM
74	sodium acetate	natural abundance	5 mM
75	LDAO	natural abundance	44 mM
76	NaCl	natural abundance	25 mM
77	D2O	natural abundance	5 %
78	DSS	natural abundance	20 uM

25 2D 1H-15N TROSY-HSQC

Instrument

Bruker AVANCE II - 900 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.25 mM [U-15N] C427-MTSL alpha7nAChR TMD+ICD, 5 mM sodium acetate, 44 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
79	C427-MTSL alpha7nAChR TMD+ICD	[U-15N]	0.25 (±0.01) mM
80	sodium acetate	natural abundance	5 mM
81	LDAO	natural abundance	44 mM
82	NaCl	natural abundance	25 mM
83	D2O	natural abundance	5 %
84	DSS	natural abundance	20 uM

26 2D 1H-15N TROSY-HSQC

Instrument

Bruker AVANCE II - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.25 mM [U-15N] C435-MTSL alpha7nAChR TMD+ICD, 5 mM sodium acetate, 44 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
85	C435-MTSL alpha7nAChR TMD+ICD	[U-15N]	0.25 (±0.01) mM
86	sodium acetate	natural abundance	5 mM
87	LDAO	natural abundance	44 mM
88	NaCl	natural abundance	25 mM
89	D2O	natural abundance	5 %
90	DSS	natural abundance	20 uM

27 2D 1H-15N TROSY-HSQC

Instrument

Bruker AVANCE II - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.28 mM V311C-MTSL alpha7 TMD-ICD, 0.07 mM [U-15N] V311C alpha7 TMD-ICD, 5 mM sodium acetate, 44 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
91	V311C-MTSL alpha7 TMD-ICD	natural abundance	0.28 (±0.01) mM
92	V311C alpha7 TMD-ICD	[U-15N]	0.07 (±0.005) mM
93	sodium acetate	natural abundance	5 mM
94	LDAO	natural abundance	44 mM
95	NaCl	natural abundance	25 mM
96	D2O	natural abundance	5 %
97	DSS	natural abundance	20 uM

28 2D 1H-15N TROSY-HSQC

Instrument

Bruker AVANCE II - 900 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.24 mM C427-MTSL alpha7 TMD-ICD, 0.06 mM [U-15N] C427 alpha7 TMD-ICD, 5 mM sodium acetate, 44 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
98	C427-MTSL alpha7 TMD-ICD	natural abundance	0.24 (±0.01) mM
99	C427 alpha7 TMD-ICD	[U-15N]	0.06 (±0.005) mM
100	sodium acetate	natural abundance	5 mM
101	LDAO	natural abundance	44 mM
102	NaCl	natural abundance	25 mM
103	D2O	natural abundance	5 %
104	DSS	natural abundance	20 uM

29 2D 1H-15N TROSY-HSQC

Instrument

Bruker AVANCE II - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.28 mM V311C alpha7 TMD-ICD, 0.07 mM [U-15N] V311C-MTSL alpha7 TMD-ICD, 5 mM sodium acetate, 44 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
105	V311C-MTSL alpha7 TMD-ICD	[U-15N]	0.07 (±0.005) mM
106	V311C alpha7 TMD-ICD	natural abundance	0.28 (±0.01) mM
107	sodium acetate	natural abundance	5 mM
108	LDAO	natural abundance	44 mM
109	NaCl	natural abundance	25 mM
110	D2O	natural abundance	5 %
111	DSS	natural abundance	20 uM

30 2D 1H-15N TROSY-HSQC

Instrument

Bruker AVANCE II - 900 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.24 mM C427 alpha7 TMD-ICD, 0.06 mM [U-15N] C427-MTSL alpha7 TMD-ICD, 5 mM sodium acetate, 44 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
112	C427-MTSL alpha7 TMD-ICD	[U-15N]	0.06 (±0.005) mM
113	C427 alpha7 TMD-ICD	natural abundance	0.24 (±0.01) mM
114	sodium acetate	natural abundance	5 mM
115	LDAO	natural abundance	44 mM
116	NaCl	natural abundance	25 mM
117	D2O	natural abundance	5 %
118	DSS	natural abundance	20 uM

31 2D 1H-15N TROSY-HSQC

Instrument

Bruker AVANCE II - 900 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 4.7, Details 0.25 mM [U-15N] C427-EDTA-Lu3+ alpha7 TMD-ICD, 5 mM sodium acetate, 53 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
119	C427-EDTA-Lu3+ alpha7 TMD-ICD	[U-15N]	0.25 (±0.01) mM
120	sodium acetate	natural abundance	5 mM
121	LDAO	natural abundance	53 mM
122	NaCl	natural abundance	25 mM
123	D2O	natural abundance	5 %
124	DSS	natural abundance	20 uM

32 2D 1H-15N IPAP

Instrument

Bruker AVANCE II - 900 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 4.7, Details 0.2 mM [U-15N] C427-EDTA-Tm3+ alpha7 TMD-ICD, 5 mM sodium acetate, 66 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
125	C427-EDTA-Tm3+ alpha7 TMD-ICD	[U-15N]	0.2 (±0.01) mM
126	sodium acetate	natural abundance	5 mM
127	LDAO	natural abundance	66 mM
128	NaCl	natural abundance	25 mM
129	D2O	natural abundance	5 %
130	DSS	natural abundance	20 uM

33 2D 1H-15N TROSY-HSQC

Instrument

Bruker AVANCE II - 900 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 4.7, Details 0.25 mM [U-15N] C317-EDTA-Lu3+ alpha7 TMD-ICD, 5 mM sodium acetate, 66 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
131	C317-EDTA-Lu3+ alpha7 TMD-ICD	[U-15N]	0.25 (±0.01) mM
132	sodium acetate	natural abundance	5 mM
133	LDAO	natural abundance	66 mM
134	NaCl	natural abundance	25 mM
135	D2O	natural abundance	5 %
136	DSS	natural abundance	20 uM

34 2D 1H-15N IPAP

Instrument

Bruker AVANCE II - 900 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 4.7, Details 0.25 mM [U-15N] C317-EDTA-Tm3+ alpha7 TMD-ICD, 5 mM sodium acetate, 53 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
137	C317-EDTA-Tm3+ alpha7 TMD-ICD	[U-15N]	0.25 (±0.01) mM
138	sodium acetate	natural abundance	5 mM
139	LDAO	natural abundance	53 mM
140	NaCl	natural abundance	25 mM
141	D2O	natural abundance	5 %
142	DSS	natural abundance	20 uM

35 2D 1H-15N TROSY-HSQC

Instrument

Bruker AVANCE II - 900 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 4.7, Details 0.24 mM [U-15N] C219-EDTA-Lu3+ alpha7 TMD-ICD, 5 mM sodium acetate, 44 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
143	C219-EDTA-Lu3+ alpha7 TMD-ICD	[U-15N]	0.24 (±0.01) mM
144	sodium acetate	natural abundance	5 mM
145	LDAO	natural abundance	44 mM
146	NaCl	natural abundance	25 mM
147	D2O	natural abundance	5 %
148	DSS	natural abundance	20 uM

36 2D 1H-15N IPAP

Instrument

Bruker AVANCE II - 900 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 4.7, Details 0.22 mM [U-15N] C219-EDTA-Tm3+ alpha7 TMD-ICD, 5 mM sodium acetate, 53 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
149	C219-EDTA-Tm3+ alpha7 TMD-ICD	[U-15N]	0.22 (±0.01) mM
150	sodium acetate	natural abundance	5 mM
151	LDAO	natural abundance	53 mM
152	NaCl	natural abundance	25 mM
153	D2O	natural abundance	5 %
154	DSS	natural abundance	20 uM

37 2D 1H-15N TROSY-HSQC

Instrument

Bruker AVANCE II - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.35 mM [U-13C; U-15N] alpha7nAChR C317 TMD+ICD, 5 mM sodium acetate, 44 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
155	alpha7nAChR C317 TMD+ICD	[U-13C; U-15N]	0.35 (±0.01) mM
156	sodium acetate	natural abundance	5 mM
157	LDAO	natural abundance	44 mM
158	NaCl	natural abundance	25 mM
159	D2O	natural abundance	5 %
160	DSS	natural abundance	20 uM

38 3D TROSY-HNCA

Instrument

Bruker AVANCE II - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
155	alpha7nAChR C317 TMD+ICD	[U-13C; U-15N]	0.35 (±0.01) mM
156	sodium acetate	natural abundance	5 mM
157	LDAO	natural abundance	44 mM
158	NaCl	natural abundance	25 mM
159	D2O	natural abundance	5 %
160	DSS	natural abundance	20 uM

39 3D TROSY-HN(CO)CA

Instrument

Bruker AVANCE II - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
155	alpha7nAChR C317 TMD+ICD	[U-13C; U-15N]	0.35 (±0.01) mM
156	sodium acetate	natural abundance	5 mM
157	LDAO	natural abundance	44 mM
158	NaCl	natural abundance	25 mM
159	D2O	natural abundance	5 %
160	DSS	natural abundance	20 uM

40 2D 1H-15N TROSY-HSQC

Instrument

Bruker AVANCE II - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 4.7, Details 0.28 mM V311C-MTSL alpha7 TMD-ICD, 0.07 mM [U-15N] V311C alpha7 TMD-ICD, 5 mM sodium acetate, 44 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
91	V311C-MTSL alpha7 TMD-ICD	natural abundance	0.28 (±0.01) mM
92	V311C alpha7 TMD-ICD	[U-15N]	0.07 (±0.005) mM
93	sodium acetate	natural abundance	5 mM
94	LDAO	natural abundance	44 mM
95	NaCl	natural abundance	25 mM
96	D2O	natural abundance	5 %
97	DSS	natural abundance	20 uM

41 2D 1H-15N TROSY-HSQC

Instrument

Bruker AVANCE II - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 4.7, Details 0.28 mM V311C alpha7 TMD-ICD, 0.07 mM [U-15N] V311C-MTSL alpha7 TMD-ICD, 5 mM sodium acetate, 44 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
105	V311C-MTSL alpha7 TMD-ICD	[U-15N]	0.07 (±0.005) mM
106	V311C alpha7 TMD-ICD	natural abundance	0.28 (±0.01) mM
107	sodium acetate	natural abundance	5 mM
108	LDAO	natural abundance	44 mM
109	NaCl	natural abundance	25 mM
110	D2O	natural abundance	5 %
111	DSS	natural abundance	20 uM

42 2D 1H-15N TROSY-HSQC

Instrument

Bruker AVANCE II - 900 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 4.7, Details 0.24 mM C427-MTSL alpha7 TMD-ICD, 0.06 mM [U-15N] C427 alpha7 TMD-ICD, 5 mM sodium acetate, 44 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
98	C427-MTSL alpha7 TMD-ICD	natural abundance	0.24 (±0.01) mM
99	C427 alpha7 TMD-ICD	[U-15N]	0.06 (±0.005) mM
100	sodium acetate	natural abundance	5 mM
101	LDAO	natural abundance	44 mM
102	NaCl	natural abundance	25 mM
103	D2O	natural abundance	5 %
104	DSS	natural abundance	20 uM

43 2D 1H-15N TROSY-HSQC

Instrument

Bruker AVANCE II - 900 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 4.7, Details 0.24 mM C427 alpha7 TMD-ICD, 0.06 mM [U-15N] C427-MTSL alpha7 TMD-ICD, 5 mM sodium acetate, 44 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
112	C427-MTSL alpha7 TMD-ICD	[U-15N]	0.06 (±0.005) mM
113	C427 alpha7 TMD-ICD	natural abundance	0.24 (±0.01) mM
114	sodium acetate	natural abundance	5 mM
115	LDAO	natural abundance	44 mM
116	NaCl	natural abundance	25 mM
117	D2O	natural abundance	5 %
118	DSS	natural abundance	20 uM

44 2D 1H-15N TROSY-HSQC

Instrument

Bruker AVANCE II - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 4.7, Details 0.24 mM [U-13C; U-15N] alpha7nAChR TMD+ICD, 5 mM sodium acetate, 66 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	alpha7nAChR TMD+ICD	[U-13C; U-15N]	0.24 (±0.01) mM
2	sodium acetate	natural abundance	5 mM
3	LDAO	natural abundance	66 mM
4	NaCl	natural abundance	25 mM
5	D2O	natural abundance	5 %
6	DSS	natural abundance	20 uM

45 3D BEST-HNCA

Instrument

Bruker AVANCE II - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	alpha7nAChR TMD+ICD	[U-13C; U-15N]	0.24 (±0.01) mM
2	sodium acetate	natural abundance	5 mM
3	LDAO	natural abundance	66 mM
4	NaCl	natural abundance	25 mM
5	D2O	natural abundance	5 %
6	DSS	natural abundance	20 uM

46 3D BEST-HN(CO)CA

Instrument

Bruker AVANCE II - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	alpha7nAChR TMD+ICD	[U-13C; U-15N]	0.24 (±0.01) mM
2	sodium acetate	natural abundance	5 mM
3	LDAO	natural abundance	66 mM
4	NaCl	natural abundance	25 mM
5	D2O	natural abundance	5 %
6	DSS	natural abundance	20 uM

47 3D TROSY-CBCACONH

Instrument

Bruker AVANCE II - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	alpha7nAChR TMD+ICD	[U-13C; U-15N]	0.24 (±0.01) mM
2	sodium acetate	natural abundance	5 mM
3	LDAO	natural abundance	66 mM
4	NaCl	natural abundance	25 mM
5	D2O	natural abundance	5 %
6	DSS	natural abundance	20 uM

48 3D BEST-HNCO

Instrument

Bruker AVANCE II - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	alpha7nAChR TMD+ICD	[U-13C; U-15N]	0.24 (±0.01) mM
2	sodium acetate	natural abundance	5 mM
3	LDAO	natural abundance	66 mM
4	NaCl	natural abundance	25 mM
5	D2O	natural abundance	5 %
6	DSS	natural abundance	20 uM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr30939_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr30939_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	OK	2113		95.6 (chain: 1, length: 272)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 272, Sequence coverage: 95.6%
- Total number of assignments
  - ¹H chemical shifts: 1110
  - ¹³C chemical shifts: 764
  - ¹⁵N chemical shifts: 239
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Keywords Cys-loop receptors, MEMBRANE PROTEIN, intracellular domains, intrinsically disordered proteins, intrinsically disordered regions, ion channels, nAChRs

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	1595	868	54.4
¹³C chemical shifts	1240	764	61.6
¹⁵N chemical shifts	283	239	84.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	543	465	85.6
¹³C chemical shifts	544	459	84.4
¹⁵N chemical shifts	254	231	90.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	1052	403	38.3
¹³C chemical shifts	696	305	43.8
¹⁵N chemical shifts	29	8	27.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	188	121	64.4
¹³C chemical shifts	188	102	54.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	114	27	23.7
¹³C chemical shifts	110	17	15.5
¹⁵N chemical shifts	4	4	100.0

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Found 1 Document (0.57 seconds)

BMRB: 30939

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Related entities 1. Neuronal acetylcholine receptor subunit alpha-7, : 1 : 1 : 233 entities Detail

Interaction partners 1. Neuronal acetylcholine receptor subunit alpha-7, : 6 interactors Detail

Experiments performed 48 experiments Detail

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