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Structures of the Intracellular Domain and Transmembrane Domain of the Human alpha7 Nicotinic Acetylcholine Receptors
Authors
Bondarenko, V., Chen, Q., Tang, P.
Assembly
Neuronal acetylcholine receptor subunit alpha-7
Entity
1. Neuronal acetylcholine receptor subunit alpha-7 (polymer), 272 monomers, 29803.50 × 5 Da Detail

SNAEEELYYG LNLLIPCVLI SALALLVFLL PADSGEKISL GITVLLSLTV FMLLVAEIMP STSDSSPSIA QYFASTMIIV GLSVVVTVIV LQYHHHDPDG GKMPKWTRVI LLNWCAWFLR MKRPGEDKVR PACQHKQRRC SLASVEMSAV APPPASNGNL LYIGFRGLDG VHCVPTPDSG VVCGRMACSP THDEHLLHGG QPPEGDPDLA KILEEVRYIA NRFRCQDESE AVCSEWKFAA CVVDRLCLMA FSVFTIICTI GILMSAPNFV EE


Total weight
149017.5 Da
Max. entity weight
29803.5 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 96.7 %, Completeness: 62.3 %, Completeness (bb): 84.8 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All62.3 % (1943 of 3118)56.4 % (900 of 1595)64.4 % (798 of 1240)86.6 % (245 of 283)
Backbone84.8 % (1353 of 1596)85.3 % (463 of 543)81.9 % (654 of 799)92.9 % (236 of 254)
Sidechain43.8 % (778 of 1777)41.5 % (437 of 1052)47.7 % (332 of 696)31.0 % (9 of 29)
Aromatic21.1 % (48 of 228)23.7 % (27 of 114)15.5 % (17 of 110)100.0 % (4 of 4)
Methyl62.3 % (223 of 358)67.6 % (121 of 179)57.0 % (102 of 179)

1. entity 1

SNAEEELYYG LNLLIPCVLI SALALLVFLL PADSGEKISL GITVLLSLTV FMLLVAEIMP STSDSSPSIA QYFASTMIIV GLSVVVTVIV LQYHHHDPDG GKMPKWTRVI LLNWCAWFLR MKRPGEDKVR PACQHKQRRC SLASVEMSAV APPPASNGNL LYIGFRGLDG VHCVPTPDSG VVCGRMACSP THDEHLLHGG QPPEGDPDLA KILEEVRYIA NRFRCQDESE AVCSEWKFAA CVVDRLCLMA FSVFTIICTI GILMSAPNFV EE

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.24 mM [U-13C; U-15N] alpha7nAChR TMD+ICD, 5 mM sodium acetate, 66 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
1alpha7nAChR TMD+ICD[U-13C; U-15N]0.24 (±0.01) mM
2sodium acetatenatural abundance5 mM
3LDAOnatural abundance66 mM
4NaClnatural abundance25 mM
5D2Onatural abundance5 %
6DSSnatural abundance20 uM
Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.25 mM [U-13C; U-15N] alpha7nAChR C317 TMD+ICD, 5 mM sodium acetate, 44 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
7alpha7nAChR C317 TMD+ICD[U-13C; U-15N]0.25 (±0.01) mM
8sodium acetatenatural abundance5 mM
9LDAOnatural abundance44 mM
10NaClnatural abundance25 mM
11D2Onatural abundance5 %
12DSSnatural abundance20 uM
Sample #3

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.25 mM [U-13C; U-15N] alpha7nAChR C317 TMD+ICD, 5 mM sodium acetate, 44 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
13alpha7nAChR C317 TMD+ICD[U-13C; U-15N]0.25 (±0.01) mM
14sodium acetatenatural abundance5 mM
15LDAOnatural abundance44 mM
16NaClnatural abundance25 mM
17D2Onatural abundance5 %
18DSSnatural abundance20 uM
Sample #4

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.35 mM [U-15N] alpha7nAChR C317 TMD+ICD, 5 mM sodium acetate, 66 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
19alpha7nAChR C317 TMD+ICD[U-15N]0.35 (±0.01) mM
20sodium acetatenatural abundance5 mM
21LDAOnatural abundance66 mM
22NaClnatural abundance25 mM
23D2Onatural abundance5 %
24DSSnatural abundance20 uM
Sample #5

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.22 mM [U-15N] V311C-MTSL alpha7nAChR TMD+ICD, 5 mM sodium acetate, 44 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
25V311C-MTSL alpha7nAChR TMD+ICD[U-15N]0.22 (±0.01) mM
26sodium acetatenatural abundance5 mM
27LDAOnatural abundance44 mM
28NaClnatural abundance25 mM
29D2Onatural abundance5 %
30DSSnatural abundance20 uM
Sample #6

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.22 mM [U-15N] C317-MTSL alpha7nAChR TMD+ICD, 5 mM sodium acetate, 44 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
31C317-MTSL alpha7nAChR TMD+ICD[U-15N]0.22 (±0.01) mM
32sodium acetatenatural abundance5 mM
33LDAOnatural abundance44 mM
34NaClnatural abundance25 mM
35D2Onatural abundance5 %
36DSSnatural abundance20 uM
Sample #7

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.3 mM [U-15N] C335-MTSL alpha7nAChR TMD+ICD, 5 mM sodium acetate, 53 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
37C335-MTSL alpha7nAChR TMD+ICD[U-15N]0.3 (±0.01) mM
38sodium acetatenatural abundance5 mM
39LDAOnatural abundance53 mM
40NaClnatural abundance25 mM
41D2Onatural abundance5 %
42DSSnatural abundance20 uM
Sample #8

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.3 mM [U-15N] C342-MTSL alpha7nAChR TMD+ICD, 5 mM sodium acetate, 66 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
43C342-MTSL alpha7nAChR TMD+ICD[U-15N]0.3 (±0.01) mM
44sodium acetatenatural abundance5 mM
45LDAOnatural abundance66 mM
46NaClnatural abundance25 mM
47D2Onatural abundance5 %
48DSSnatural abundance20 uM
Sample #9

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.3 mM [U-15N] S350C-MTSL alpha7nAChR TMD+ICD, 5 mM sodium acetate, 53 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
49S350C-MTSL alpha7nAChR TMD+ICD[U-15N]0.3 (±0.01) mM
50sodium acetatenatural abundance5 mM
51LDAOnatural abundance53 mM
52NaClnatural abundance25 mM
53D2Onatural abundance5 %
54DSSnatural abundance20 uM
Sample #10

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.25 mM [U-15N] S358C-MTSL alpha7nAChR TMD+ICD, 5 mM sodium acetate, 44 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
55S358C-MTSL alpha7nAChR TMD+ICD[U-15N]0.25 (±0.01) mM
56sodium acetatenatural abundance5 mM
57LDAOnatural abundance44 mM
58NaClnatural abundance25 mM
59D2Onatural abundance5 %
60DSSnatural abundance20 uM
Sample #11

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.25 mM [U-15N] C375-MTSL alpha7nAChR TMD+ICD, 5 mM sodium acetate, 66 mM LDAO, 20 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
61C375-MTSL alpha7nAChR TMD+ICD[U-15N]0.25 (±0.01) mM
62sodium acetatenatural abundance5 mM
63LDAOnatural abundance66 mM
64NaClnatural abundance20 mM
65D2Onatural abundance5 %
66DSSnatural abundance20 uM
Sample #12

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.22 mM [U-15N] C390-MTSL alpha7nAChR TMD+ICD, 5 mM sodium acetate, 53 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
67C390-MTSL alpha7nAChR TMD+ICD[U-15N]0.22 (±0.01) mM
68sodium acetatenatural abundance5 mM
69LDAOnatural abundance53 mM
70NaClnatural abundance25 mM
71D2Onatural abundance5 %
72DSSnatural abundance20 uM
Sample #13

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.25 mM [U-15N] L415C-MTSL alpha7nAChR TMD+ICD, 5 mM sodium acetate, 44 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
73L415C-MTSL alpha7nAChR TMD+ICD[U-15N]0.25 (±0.01) mM
74sodium acetatenatural abundance5 mM
75LDAOnatural abundance44 mM
76NaClnatural abundance25 mM
77D2Onatural abundance5 %
78DSSnatural abundance20 uM
Sample #14

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.25 mM [U-15N] C427-MTSL alpha7nAChR TMD+ICD, 5 mM sodium acetate, 44 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
79C427-MTSL alpha7nAChR TMD+ICD[U-15N]0.25 (±0.01) mM
80sodium acetatenatural abundance5 mM
81LDAOnatural abundance44 mM
82NaClnatural abundance25 mM
83D2Onatural abundance5 %
84DSSnatural abundance20 uM
Sample #15

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.25 mM [U-15N] C435-MTSL alpha7nAChR TMD+ICD, 5 mM sodium acetate, 44 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
85C435-MTSL alpha7nAChR TMD+ICD[U-15N]0.25 (±0.01) mM
86sodium acetatenatural abundance5 mM
87LDAOnatural abundance44 mM
88NaClnatural abundance25 mM
89D2Onatural abundance5 %
90DSSnatural abundance20 uM
Sample #16

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.28 mM V311C-MTSL alpha7 TMD-ICD, 0.07 mM [U-15N] V311C alpha7 TMD-ICD, 5 mM sodium acetate, 44 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
91V311C-MTSL alpha7 TMD-ICDnatural abundance0.28 (±0.01) mM
92V311C alpha7 TMD-ICD[U-15N]0.07 (±0.005) mM
93sodium acetatenatural abundance5 mM
94LDAOnatural abundance44 mM
95NaClnatural abundance25 mM
96D2Onatural abundance5 %
97DSSnatural abundance20 uM
Sample #17

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.24 mM C427-MTSL alpha7 TMD-ICD, 0.06 mM [U-15N] C427 alpha7 TMD-ICD, 5 mM sodium acetate, 44 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
98C427-MTSL alpha7 TMD-ICDnatural abundance0.24 (±0.01) mM
99C427 alpha7 TMD-ICD[U-15N]0.06 (±0.005) mM
100sodium acetatenatural abundance5 mM
101LDAOnatural abundance44 mM
102NaClnatural abundance25 mM
103D2Onatural abundance5 %
104DSSnatural abundance20 uM
Sample #18

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.28 mM V311C alpha7 TMD-ICD, 0.07 mM [U-15N] V311C-MTSL alpha7 TMD-ICD, 5 mM sodium acetate, 44 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
105V311C-MTSL alpha7 TMD-ICD[U-15N]0.07 (±0.005) mM
106V311C alpha7 TMD-ICDnatural abundance0.28 (±0.01) mM
107sodium acetatenatural abundance5 mM
108LDAOnatural abundance44 mM
109NaClnatural abundance25 mM
110D2Onatural abundance5 %
111DSSnatural abundance20 uM
Sample #19

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.24 mM C427 alpha7 TMD-ICD, 0.06 mM [U-15N] C427-MTSL alpha7 TMD-ICD, 5 mM sodium acetate, 44 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
112C427-MTSL alpha7 TMD-ICD[U-15N]0.06 (±0.005) mM
113C427 alpha7 TMD-ICDnatural abundance0.24 (±0.01) mM
114sodium acetatenatural abundance5 mM
115LDAOnatural abundance44 mM
116NaClnatural abundance25 mM
117D2Onatural abundance5 %
118DSSnatural abundance20 uM
Sample #20

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.25 mM [U-15N] C427-EDTA-Lu3+ alpha7 TMD-ICD, 5 mM sodium acetate, 53 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
119C427-EDTA-Lu3+ alpha7 TMD-ICD[U-15N]0.25 (±0.01) mM
120sodium acetatenatural abundance5 mM
121LDAOnatural abundance53 mM
122NaClnatural abundance25 mM
123D2Onatural abundance5 %
124DSSnatural abundance20 uM
Sample #21

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.2 mM [U-15N] C427-EDTA-Tm3+ alpha7 TMD-ICD, 5 mM sodium acetate, 66 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
125C427-EDTA-Tm3+ alpha7 TMD-ICD[U-15N]0.2 (±0.01) mM
126sodium acetatenatural abundance5 mM
127LDAOnatural abundance66 mM
128NaClnatural abundance25 mM
129D2Onatural abundance5 %
130DSSnatural abundance20 uM
Sample #22

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.25 mM [U-15N] C317-EDTA-Lu3+ alpha7 TMD-ICD, 5 mM sodium acetate, 66 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
131C317-EDTA-Lu3+ alpha7 TMD-ICD[U-15N]0.25 (±0.01) mM
132sodium acetatenatural abundance5 mM
133LDAOnatural abundance66 mM
134NaClnatural abundance25 mM
135D2Onatural abundance5 %
136DSSnatural abundance20 uM
Sample #23

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.25 mM [U-15N] C317-EDTA-Tm3+ alpha7 TMD-ICD, 5 mM sodium acetate, 53 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
137C317-EDTA-Tm3+ alpha7 TMD-ICD[U-15N]0.25 (±0.01) mM
138sodium acetatenatural abundance5 mM
139LDAOnatural abundance53 mM
140NaClnatural abundance25 mM
141D2Onatural abundance5 %
142DSSnatural abundance20 uM
Sample #24

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.24 mM [U-15N] C219-EDTA-Lu3+ alpha7 TMD-ICD, 5 mM sodium acetate, 44 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
143C219-EDTA-Lu3+ alpha7 TMD-ICD[U-15N]0.24 (±0.01) mM
144sodium acetatenatural abundance5 mM
145LDAOnatural abundance44 mM
146NaClnatural abundance25 mM
147D2Onatural abundance5 %
148DSSnatural abundance20 uM
Sample #25

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.22 mM [U-15N] C219-EDTA-Tm3+ alpha7 TMD-ICD, 5 mM sodium acetate, 53 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
149C219-EDTA-Tm3+ alpha7 TMD-ICD[U-15N]0.22 (±0.01) mM
150sodium acetatenatural abundance5 mM
151LDAOnatural abundance53 mM
152NaClnatural abundance25 mM
153D2Onatural abundance5 %
154DSSnatural abundance20 uM
Sample #26

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.7, Details 0.35 mM [U-13C; U-15N] alpha7nAChR C317 TMD+ICD, 5 mM sodium acetate, 44 mM LDAO, 25 mM NaCl, 5 % D2O, 20 uM DSS, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
155alpha7nAChR C317 TMD+ICD[U-13C; U-15N]0.35 (±0.01) mM
156sodium acetatenatural abundance5 mM
157LDAOnatural abundance44 mM
158NaClnatural abundance25 mM
159D2Onatural abundance5 %
160DSSnatural abundance20 uM

Release date
2021-08-07
Citation
Structures of highly flexible intracellular domain of human α7 nicotinic acetylcholine receptor
Bondarenko, V., Wells, M.M., Chen, Q., Tillman, T.S., Singewald, K., Lawless, M.J., Caporoso, J., Brandon, N., Coleman, J.A., Saxena, S., Lindahl, E., Xu, Y., Tang, P.
Nat. Commun. (2022), 13, 793-793, PubMed 35145092 , DOI 10.1038/s41467-022-28400-x ,
Related entities 1. Neuronal acetylcholine receptor subunit alpha-7, : 1 : 1 : 233 entities Detail
Interaction partners 1. Neuronal acetylcholine receptor subunit alpha-7, : 6 interactors Detail
Experiments performed 48 experiments Detail
Chemical shift validation 3 contents Detail
Keywords Cys-loop receptors, MEMBRANE PROTEIN, intracellular domains, intrinsically disordered proteins, intrinsically disordered regions, ion channels, nAChRs