BMRB: 30948

NMR Solution Structure of hGal(1-12)KK, a solubility-tagged truncation of the human neuropeptide galanin

Authors

Kraichely, K.N., Mendoza, E.A., Parnham, S., Giuliano, M.W.

Assembly

Galanin

Entity

1. Galanin (polymer, Thiol state: not present), 19 monomers, 1839.082 Da Detail

GWTLNSAGYL LGPHAVGKX

Formula weight

1839.082 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 94.7 %, Completeness: 100.0 %, Completeness (bb): 100.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	100.0 % (99 of 99)	100.0 % (99 of 99)
Backbone	100.0 % (39 of 39)	100.0 % (39 of 39)
Sidechain	100.0 % (60 of 60)	100.0 % (60 of 60)
Aromatic	100.0 % (12 of 12)	100.0 % (12 of 12)
Methyl	100.0 % (11 of 11)	100.0 % (11 of 11)

1. entity 1

GWTLNSAGYL LGPHAVGKX

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 4.6, Details 2 mM hGal(1-17)K, 4.14 mM D3 sodium acetate, 5.86 mM D4 acetic acid, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	hGal(1-17)K	natural abundance		2 mM
2	sodium acetate	D3		4.14 mM
3	acetic acid	D4		5.86 mM

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Release date

2021-09-25

Citation

Minimal Increments of Hydrophobic Collapse within the N-Terminus of the Neuropeptide Galanin
Kraichely, K.N., Clinkscales, S.E., Hendy, C.M., Mendoza, E.A., Parnham, S., Giuliano, M.W.
Biochemistry (2022), 61, 1151-1166, PubMed 35622960 , DOI 10.1021/acs.biochem.2c00141 , Abstract

Related entities 1. Galanin, : 5 : 13 entities Detail

Interaction partners 1. Galanin, : 5 interactors Detail

Experiments performed 4 experiments Detail