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In response to the declaration of global pandemic of Coronavirus infectious disease 2019 (COVID-19) caused by Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), we provide comprehensive content on Coronavirus molecular structures and its interactions:

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BMRB: 30955

7SD4

SARS-CoV-2 Nucleocapsid N-terminal domain (N-NTD) protein

Authors

Sarkar, S., Runge, B., Russell, R.W., Calero, D., Zeinalilathori, S., Quinn, C.M., Lu, M., Calero, G., Gronenborn, A.M., Polenova, T.

Assembly

Nucleoprotein

Entity

1. Nucleoprotein (polymer, Thiol state: not present), 136 monomers, 14975.57 Da Detail

SRRPQGLPNN TASWFTALTQ HGKEDLKFPR GQGVPINTNS SPDDQIGYYR RATRRIRGGD GKMKDLSPRW YFYYLGTGPE AGLPYGANKD GIIWVATEGA LNTPKDHIGT RNPANNAAIV LQLPQGTTLP KGFYAE

Formula weight

14975.57 Da

Source organism

Severe acute respiratory syndrome coronavirus 2

Exptl. method

solid-state NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.8 %, Completeness: 52.0 %, Completeness (bb): 77.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	52.0 % (819 of 1576)	16.2 % (133 of 821)	90.9 % (548 of 603)	90.8 % (138 of 152)
Backbone	77.4 % (613 of 792)	42.2 % (117 of 277)	95.9 % (375 of 391)	97.6 % (121 of 124)
Sidechain	35.4 % (320 of 903)	2.9 % (16 of 544)	86.7 % (287 of 331)	60.7 % (17 of 28)
Aromatic	40.0 % (56 of 140)	4.3 % (3 of 70)	74.6 % (50 of 67)	100.0 % (3 of 3)
Methyl	42.7 % (53 of 124)	0.0 % (0 of 62)	85.5 % (53 of 62)

1. entity 1

SRRPQGLPNN TASWFTALTQ HGKEDLKFPR GQGVPINTNS SPDDQIGYYR RATRRIRGGD GKMKDLSPRW YFYYLGTGPE AGLPYGANKD GIIWVATEGA LNTPKDHIGT RNPANNAAIV LQLPQGTTLP KGFYAE

Show sample condition

Sample

Solvent system water, Pressure 1 atm, Temperature 298.15 K, pH 6.2, Details 30 mg/mL [U-100% 13C; U-100% 15N] N-NTD, water

#	Name	Isotope labeling	Type	Concentration
1	N-NTD	[U-100% 13C; U-100% 15N]		30 mg/mL

Release date

2021-10-14

Citation

Atomic-Resolution Structure of SARS-CoV-2 Nucleocapsid Protein N-Terminal Domain
Sarkar, S., Runge, B., Russell, R.W., Movellan, K.T., Calero, D., Zeinalilathori, S., Quinn, C.M., Lu, M., Calero, G., Gronenborn, A.M., Polenova, T.
J. Am. Chem. Soc. (2022), 144, 10543-10555, PubMed 35638584 , DOI 10.1021/jacs.2c03320 , Abstract

Related entities 1. Nucleoprotein, : 1 : 1 : 27 : 64 entities Detail

Interaction partners 1. Nucleoprotein, : 3 interactors Detail

More details for 3 interactors identified by 2 citations

Experiments performed 11 experiments Detail

1 2D CORD 25 ms

Instrument

Bruker AVANCE III - 600 MHz

Sample

State isotropic, Solvent system water, Pressure 1 atm, Temperature 298.15 K, pH 6.2, Details 30 mg/mL [U-100% 13C; U-100% 15N] N-NTD, water

#	Name	Isotope labeling	Type	Concentration
1	N-NTD	[U-100% 13C; U-100% 15N]		30 mg/mL

2 2D CORD 100 ms

Instrument

Bruker AVANCE III - 600 MHz

Sample

State isotropic, Solvent system water, Pressure 1 atm, Temperature 298.15 K, pH 6.2, Details 30 mg/mL [U-100% 13C; U-100% 15N] N-NTD, water

#	Name	Isotope labeling	Type	Concentration
1	N-NTD	[U-100% 13C; U-100% 15N]		30 mg/mL

3 2D CORD 250 ms

Instrument

Bruker AVANCE III - 600 MHz

Sample

State isotropic, Solvent system water, Pressure 1 atm, Temperature 298.15 K, pH 6.2, Details 30 mg/mL [U-100% 13C; U-100% 15N] N-NTD, water

#	Name	Isotope labeling	Type	Concentration
1	N-NTD	[U-100% 13C; U-100% 15N]		30 mg/mL

4 2D CORD 500 ms

Instrument

Bruker AVANCE III - 600 MHz

Sample

State isotropic, Solvent system water, Pressure 1 atm, Temperature 298.15 K, pH 6.2, Details 30 mg/mL [U-100% 13C; U-100% 15N] N-NTD, water

#	Name	Isotope labeling	Type	Concentration
1	N-NTD	[U-100% 13C; U-100% 15N]		30 mg/mL

5 2D NCACX 25 ms

Instrument

Bruker AVANCE III - 600 MHz

Sample

State isotropic, Solvent system water, Pressure 1 atm, Temperature 298.15 K, pH 6.2, Details 30 mg/mL [U-100% 13C; U-100% 15N] N-NTD, water

#	Name	Isotope labeling	Type	Concentration
1	N-NTD	[U-100% 13C; U-100% 15N]		30 mg/mL

6 2D NCOCX 25 ms

Instrument

Bruker AVANCE III - 600 MHz

Sample

State isotropic, Solvent system water, Pressure 1 atm, Temperature 298.15 K, pH 6.2, Details 30 mg/mL [U-100% 13C; U-100% 15N] N-NTD, water

#	Name	Isotope labeling	Type	Concentration
1	N-NTD	[U-100% 13C; U-100% 15N]		30 mg/mL

7 2D CP RFDR

Instrument

Bruker AVANCE III - 600 MHz

Sample

State isotropic, Solvent system water, Pressure 1 atm, Temperature 298.15 K, pH 6.2, Details 30 mg/mL [U-100% 13C; U-100% 15N] N-NTD, water

#	Name	Isotope labeling	Type	Concentration
1	N-NTD	[U-100% 13C; U-100% 15N]		30 mg/mL

8 2D hNH HETCOR

Instrument

Bruker AVANCE III - 600 MHz

Sample

State isotropic, Solvent system water, Pressure 1 atm, Temperature 298.15 K, pH 6.2, Details 30 mg/mL [U-100% 13C; U-100% 15N] N-NTD, water

#	Name	Isotope labeling	Type	Concentration
1	N-NTD	[U-100% 13C; U-100% 15N]		30 mg/mL

9 2D hCH HETCOR

Instrument

Bruker AVANCE III - 600 MHz

Sample

State isotropic, Solvent system water, Pressure 1 atm, Temperature 298.15 K, pH 6.2, Details 30 mg/mL [U-100% 13C; U-100% 15N] N-NTD, water

#	Name	Isotope labeling	Type	Concentration
1	N-NTD	[U-100% 13C; U-100% 15N]		30 mg/mL

10 3D hCANH

Instrument

Bruker AVANCE III - 600 MHz

Sample

State isotropic, Solvent system water, Pressure 1 atm, Temperature 298.15 K, pH 6.2, Details 30 mg/mL [U-100% 13C; U-100% 15N] N-NTD, water

#	Name	Isotope labeling	Type	Concentration
1	N-NTD	[U-100% 13C; U-100% 15N]		30 mg/mL

11 3D hCONH

Instrument

Bruker AVANCE III - 600 MHz

Sample

State isotropic, Solvent system water, Pressure 1 atm, Temperature 298.15 K, pH 6.2, Details 30 mg/mL [U-100% 13C; U-100% 15N] N-NTD, water

#	Name	Isotope labeling	Type	Concentration
1	N-NTD	[U-100% 13C; U-100% 15N]		30 mg/mL

nullKeywords COVID-19, N-terminal domain, RNA-binding domain, SARS-CoV-2, STRUCTURAL PROTEIN, nucleocapsid protein