Solution NMR Structure of Immunoglobulin-like Domain of Human Neuregulin-1
MHHHHHHSSG RENLYFQGAL PPRLKEMKSQ ESAAGSKLVL RCETSSEYSS LRFKWFKNGN ELNRKNKPQN IKIQKKPGKS ELRINKASLA DSGEYMCKVI SKLGNDSASA NITIVESN
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 93.2 % (1310 of 1406) | 93.3 % (693 of 743) | 93.4 % (493 of 528) | 91.9 % (124 of 135) |
Backbone | 93.9 % (657 of 700) | 93.7 % (224 of 239) | 93.9 % (326 of 347) | 93.9 % (107 of 114) |
Sidechain | 92.8 % (758 of 817) | 93.1 % (469 of 504) | 93.2 % (272 of 292) | 81.0 % (17 of 21) |
Aromatic | 73.3 % (66 of 90) | 73.3 % (33 of 45) | 72.7 % (32 of 44) | 100.0 % (1 of 1) |
Methyl | 100.0 % (94 of 94) | 100.0 % (47 of 47) | 100.0 % (47 of 47) |
1. entity 1
MHHHHHHSSG RENLYFQGAL PPRLKEMKSQ ESAAGSKLVL RCETSSEYSS LRFKWFKNGN ELNRKNKPQN IKIQKKPGKS ELRINKASLA DSGEYMCKVI SKLGNDSASA NITIVESNSolvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 2.0 mM [U-13C; U-15N] NRG1-Ig, 20 mM sodium phosphate, 100 mM sodium chloride, 4 uM DSS, 0.05 % w/w sodium azide, 93% H2O/7% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | NRG1-Ig | [U-13C; U-15N] | 2.0 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | sodium chloride | natural abundance | 100 mM | |
4 | DSS | natural abundance | 4 uM | |
5 | sodium azide | natural abundance | 0.05 % w/w |
Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.45 mM [U-13C; U-15N] NRG1-Ig, 20 mM sodium phosphate, 100 mM sodium chloride, 4 uM DSS, 0.05 % w/w sodium azide, 93% H2O/7% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | NRG1-Ig | [U-13C; U-15N] | 0.45 mM | |
7 | sodium phosphate | natural abundance | 20 mM | |
8 | sodium chloride | natural abundance | 100 mM | |
9 | DSS | natural abundance | 4 uM | |
10 | sodium azide | natural abundance | 0.05 % w/w |
Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.34 mM [5% 13C; U-15N] NRG1-Ig, 20 mM TRIS, 300 mM sodium chloride, 5 uM DSS, 0.05 % w/w sodium azide, 93% H2O/7% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
11 | NRG1-Ig | [5% 13C; U-15N] | 0.34 mM | |
12 | sodium chloride | natural abundance | 300 mM | |
13 | DSS | natural abundance | 5 uM | |
14 | sodium azide | natural abundance | 0.05 % w/w | |
15 | TRIS | natural abundance | 20 mM |