BMRB: 30972

Solution structure of 7SK stem-loop 1 with HIV-1 Tat Subtype G Arginine Rich Motif

Authors

Pham, V.V., Gao, M., D'Souza, V.M.

Assembly

Tat Subtype G Arginine Rich Motif/RNA Complex

Entity

1. Tat Subtype G Arginine Rich Motif/RNA Complex, entity 1 (polymer, Thiol state: not present), 56 monomers, 18049.61 Da Detail

GGGAUCUGUC ACCCCAUUGA UCGCCAGUGG CUGAUCUGGC UGGCUAGGCG GGUCCC

2. Tat Subtype G Arginine Rich Motif/RNA Complex, entity 2 (polymer, Thiol state: not present), 17 monomers, 2162.470 Da Detail

GISYGRKKRR HRRRAHQ

Total weight

20212.08 Da

Max. entity weight

18049.61 Da

Source organism

Homo sapiens , HIV-1 06TG.HT008

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.3 %, Completeness: 36.1 %, Completeness (bb): 19.4 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polyribonucleotide polypeptide(L)

	Total	1H
All	36.1 % (223 of 617)	36.1 % (223 of 617)
Backbone	19.4 % (72 of 372)	19.4 % (72 of 372)
Sidechain	61.6 % (151 of 245)	61.6 % (151 of 245)
Aromatic	89.5 % (119 of 133)	89.5 % (119 of 133)
Methyl	0.0 % (0 of 3)	0.0 % (0 of 3)

1. entity 1

GGGAUCUGUC ACCCCAUUGA UCGCCAGUGG CUGAUCUGGC UGGCUAGGCG GGUCCC

2. entity 2

GISYGRKKRR HRRRAHQ

Show sample condition

Sample #1

Solvent system 100% D2O, Pressure 100000 Pa, Temperature 298 K, pH 5.6, Details 0.5 mM Tat G, 0.5 mM 7SK, 100% D2O

#	Name	Isotope labeling	Type	Concentration
1	Tat G	natural abundance		0.5 (±0.2) mM
2	7SK	natural abundance		0.5 (±0.2) mM
3	Phosphate	natural abundance		10 mM
4	NaCl	natural abundance		70 mM
5	EDTA	natural abundance		0.1 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 100000 Pa, Temperature 298 K, pH 5.6, Details 0.5 mM Tat G, 0.5 mM 7SK, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
6	Tat G	natural abundance		0.5 (±0.2) mM
7	7SK	natural abundance		0.5 (±0.2) mM
8	Phosphate	natural abundance		10 mM
9	NaCl	natural abundance		70 mM
10	EDTA	natural abundance		0.1 mM

Hide sample codition

Release date

2022-02-01

Citation

A structure-based mechanism for displacement of the HEXIM adapter from 7SK small nuclear RNA
Pham, V.V., Gao, M., Meagher, J.L., Smith, J.L., D'Souza, V.M.
Commun. Biol. (2022), 5, 819-819, PubMed 35970937 , DOI 10.1038/s42003-022-03734-w , Abstract

Related entities 1. Tat Subtype G Arginine Rich Motif/RNA Complex, entity 1, : 1 : 2 : 6 entities Detail

Related entities 2. Tat Subtype G Arginine Rich Motif/RNA Complex, entity 2, : 1 : 7 entities Detail

Experiments performed 2 experiments Detail