BMRB: 30986

NMR solution structure of the phosphorylated MUS81-binding region from human SLX4

Authors

Payliss, B.J., Reichheld, S.E., Lemak, A., Sharpe, S., Arrowsmith, C.H., Wyatt, H.D.M.

Assembly

Structure-specific endonuclease subunit SLX4

Entity

1. Structure-specific endonuclease subunit SLX4 (polymer, Thiol state: not present), 86 monomers, 9941.076 Da Detail

AQMPSAGGAQ KPEGLEXPKG ANRKKNLPPK VPIXPMPQYS IMEXPVLKKE LDRFGVRPLP KRQMVLKLKE IFQYTHQTLD SDSEDE

Formula weight

9941.076 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 96.5 %, Completeness: 86.0 %, Completeness (bb): 96.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	86.0 % (876 of 1019)	82.4 % (451 of 547)	90.2 % (350 of 388)	89.3 % (75 of 84)
Backbone	96.2 % (458 of 476)	93.1 % (149 of 160)	97.5 % (238 of 244)	98.6 % (71 of 72)
Sidechain	79.4 % (493 of 621)	78.0 % (302 of 387)	84.2 % (187 of 222)	33.3 % (4 of 12)
Aromatic	100.0 % (40 of 40)	100.0 % (20 of 20)	100.0 % (20 of 20)
Methyl	91.7 % (66 of 72)	91.7 % (33 of 36)	91.7 % (33 of 36)

1. entity 1

AQMPSAGGAQ KPEGLEXPKG ANRKKNLPPK VPIXPMPQYS IMEXPVLKKE LDRFGVRPLP KRQMVLKLKE IFQYTHQTLD SDSEDE

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Sample

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 283 K, pH 7.4, Details 500 uM [U-100% 13C; U-100% 15N] Phosphorylated MUS81-binding region of human SLX4, 25 mM sodium phosphate, 100 mM sodium chloride, 100 uM EDTA, 100 uM sodium azide, 93% H2O/7% D2O.

#	Name	Isotope labeling	Type	Concentration
1	Phosphorylated MUS81-binding region of human SLX4	[U-100% 13C; U-100% 15N]		500 uM
2	sodium phosphate	natural abundance		25 mM
3	sodium chloride	natural abundance		100 mM
4	EDTA	natural abundance		100 uM
5	sodium azide	natural abundance		100 uM

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Release date

2022-02-03

Citation

Phosphorylation of the DNA repair scaffold SLX4 drives folding of the SAP domain and activation of the MUS81-EME1 endonuclease
Payliss, B.J., Tse, Y., Reichheld, S.E., Lemak, A., Yun, H.Y., Houliston, S., Patel, A., Arrowsmith, C.H., Sharpe, S., Wyatt, H.
Cell Rep. (2022), 41, 111537-111537, PubMed 36288699 , DOI 10.1016/j.celrep.2022.111537 , Abstract

Related entities 1. Structure-specific endonuclease subunit SLX4, : 2 : 3 entities Detail

Interaction partners 1. Structure-specific endonuclease subunit SLX4, : 24 interactors Detail

Experiments performed 13 experiments Detail