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NMR Solution Structure of C-terminally amidated, Full-length Human Galanin
Authors
Wilkinson, R.E., Kraichely, K.N., Buchanan, L.E., Parnham, S., Giuliano, M.W.
Assembly
Galanin
Entity
1. Galanin (polymer, Thiol state: not present), 31 monomers, 3155.420 Da Detail

GWTLNSAGYL LGPHAVGNHR SFSDKNGLTS X


Formula weight
3155.42 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Data set
assigned_chemical_shifts, spectral_peak_list
Chem. Shift Complete
Sequence coverage: 96.8 %, Completeness: 97.0 %, Completeness (bb): 92.2 % Detail

Polymer type: polypeptide(L)

Total1H
All97.0 % (160 of 165)97.0 % (160 of 165)
Backbone92.2 % (59 of 64)92.2 % (59 of 64)
Sidechain100.0 % (101 of 101)100.0 % (101 of 101)
Aromatic100.0 % (19 of 19)100.0 % (19 of 19)
Methyl100.0 % (14 of 14)100.0 % (14 of 14)

1. entity 1

GWTLNSAGYL LGPHAVGNHR SFSDKNGLTS X

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 4.6 (±0.02), Details 0.5 mM hGal(1-30)NH2, 4.14 mM [U-2H] sodium acetate, 5.86 mM [U-2H] acetic acid, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
1hGal(1-30)NH2natural abundance0.5 mM
2sodium acetate[U-2H]4.14 mM
3acetic acid[U-2H]5.86 mM

Release date
2022-07-06
Citation
The neuropeptide galanin adopts an irregular secondary structure
Wilkinson, R.E., Kraichely, K.N., Hendy, C.M., Buchanan, L.E., Parnham, S., Giuliano, M.W.
Biochem. Biophys. Res. Commun. (2022), 626, 121-128, PubMed 35994823 , DOI 10.1016/j.bbrc.2022.08.032 ,
Entries sharing articles BMRB: 1 entries Detail
  BMRB: 31029 released on 2022-06-29
    Title NMR Structure of Ac-hGal(17-30)NH2, an N-terminally acetylated fragment of the C-terminus of human galanin
Related entities 1. Galanin, : 1 : 7 : 26 entities Detail
Interaction partners 1. Galanin, : 5 interactors Detail
Experiments performed 4 experiments Detail
Chemical shift validation 3 contents Detail
Keywords neuropeptide, synthetic