BMRB: 31032

NMR Solution Structure of C-terminally amidated, Full-length Human Galanin

Authors

Wilkinson, R.E., Kraichely, K.N., Buchanan, L.E., Parnham, S., Giuliano, M.W.

Assembly

Galanin

Entity

1. Galanin (polymer, Thiol state: not present), 31 monomers, 3155.420 Da Detail

GWTLNSAGYL LGPHAVGNHR SFSDKNGLTS X

Formula weight

3155.42 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 96.8 %, Completeness: 97.0 %, Completeness (bb): 92.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	97.0 % (160 of 165)	97.0 % (160 of 165)
Backbone	92.2 % (59 of 64)	92.2 % (59 of 64)
Sidechain	100.0 % (101 of 101)	100.0 % (101 of 101)
Aromatic	100.0 % (19 of 19)	100.0 % (19 of 19)
Methyl	100.0 % (14 of 14)	100.0 % (14 of 14)

1. entity 1

GWTLNSAGYL LGPHAVGNHR SFSDKNGLTS X

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 4.6 (±0.02), Details 0.5 mM hGal(1-30)NH2, 4.14 mM [U-2H] sodium acetate, 5.86 mM [U-2H] acetic acid, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	hGal(1-30)NH2	natural abundance		0.5 mM
2	sodium acetate	[U-2H]		4.14 mM
3	acetic acid	[U-2H]		5.86 mM

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Release date

2022-07-06

Citation

The neuropeptide galanin adopts an irregular secondary structure
Wilkinson, R.E., Kraichely, K.N., Hendy, C.M., Buchanan, L.E., Parnham, S., Giuliano, M.W.
Biochem. Biophys. Res. Commun. (2022), 626, 121-128, PubMed 35994823 , DOI 10.1016/j.bbrc.2022.08.032 , Abstract

Related entities 1. Galanin, : 1 : 7 : 26 entities Detail

Interaction partners 1. Galanin, : 5 interactors Detail

Experiments performed 4 experiments Detail

Chemical shift validation 3 contents Detail