BMRB: 31151

NMR solution structure of cell-permeant miniature protein ZF5.3

Authors

Giudice, J.A., Kelly, M., Schepartz, A.

Assembly

Designed Zinc finger protein 5.3

Entity

1. Designed Zinc finger protein 5.3, entity 1 (polymer), 27 monomers, 3194.702 Da Detail

YSCNVCGKAF VLSRHLNRHL RVHRRAT

2. Designed Zinc finger protein 5.3, entity ZN (non-polymer), 65.409 Da

Total weight

3260.1108 Da

Max. entity weight

3194.702 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chemcal Shifts

¹H: 198, ¹³C: 93, ¹⁵N: 32 assignments Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

ID	Entity ID	Seq ID	Comp ID	Atom ID	Atom type	Val	Val err	Ambig. code
1	1	1	TYR	H	H	7.111	0.020	1
2	1	1	TYR	HA	H	4.260	0.020	1
3	1	1	TYR	HB2	H	2.876	0.020	2
4	1	1	TYR	HB3	H	3.349	0.020	2
5	1	1	TYR	HD1	H	7.027	0.020	1
6	1	1	TYR	HD2	H	7.109	0.020	1
7	1	1	TYR	HE1	H	7.042	0.020	1
8	1	1	TYR	HE2	H	7.220	0.020	1
9	1	1	TYR	CA	C	58.042	0.400	1
10	1	1	TYR	CB	C	39.680	0.400	1
11	1	1	TYR	CD1	C	133.864	0.400	3
12	1	1	TYR	CE1	C	119.364	0.400	3
13	1	1	TYR	N	N	122.708	0.400	1
14	1	2	SER	H	H	9.294	0.020	1
15	1	2	SER	HA	H	5.465	0.020	1
16	1	2	SER	HB2	H	3.647	0.020	2
17	1	2	SER	HB3	H	3.701	0.020	2
18	1	2	SER	CA	C	57.411	0.400	1
19	1	2	SER	CB	C	65.334	0.400	1
20	1	2	SER	N	N	118.853	0.400	1
21	1	3	CYS	H	H	9.195	0.020	1
22	1	3	CYS	HA	H	4.534	0.020	1
23	1	3	CYS	HB2	H	2.921	0.020	2
24	1	3	CYS	HB3	H	3.389	0.020	2
25	1	3	CYS	CA	C	59.751	0.400	1
26	1	3	CYS	CB	C	29.915	0.400	1
27	1	3	CYS	N	N	125.650	0.400	1
28	1	4	ASN	H	H	9.315	0.020	1
29	1	4	ASN	HA	H	4.547	0.020	1
30	1	4	ASN	HB2	H	2.927	0.020	2
31	1	4	ASN	HB3	H	2.995	0.020	2
32	1	4	ASN	HD21	H	6.860	0.020	2
33	1	4	ASN	HD22	H	7.640	0.020	2
34	1	4	ASN	CA	C	55.262	0.400	1
35	1	4	ASN	CB	C	37.854	0.400	1
36	1	4	ASN	N	N	130.201	0.400	1
37	1	4	ASN	ND2	N	112.419	0.400	1
38	1	5	VAL	H	H	9.098	0.020	1
39	1	5	VAL	HA	H	3.832	0.020	1
40	1	5	VAL	HB	H	1.182	0.020	1
41	1	5	VAL	HG11	H	0.330	0.020	2
42	1	5	VAL	HG12	H	0.330	0.020	2
43	1	5	VAL	HG13	H	0.330	0.020	2
44	1	5	VAL	HG21	H	0.775	0.020	2
45	1	5	VAL	HG22	H	0.775	0.020	2
46	1	5	VAL	HG23	H	0.775	0.020	2
47	1	5	VAL	CA	C	65.108	0.400	1
48	1	5	VAL	CB	C	32.781	0.400	1
49	1	5	VAL	CG1	C	20.257	0.400	2
50	1	5	VAL	CG2	C	20.907	0.400	2
51	1	5	VAL	N	N	122.995	0.400	1
52	1	6	CYS	H	H	8.460	0.020	1
53	1	6	CYS	HA	H	5.168	0.020	1
54	1	6	CYS	HB2	H	2.820	0.020	2
55	1	6	CYS	HB3	H	2.821	0.020	2
56	1	6	CYS	CA	C	58.301	0.400	1
57	1	6	CYS	CB	C	32.716	0.400	1
58	1	6	CYS	N	N	116.907	0.400	1
59	1	7	GLY	H	H	8.032	0.020	1
60	1	7	GLY	HA2	H	3.913	0.020	2
61	1	7	GLY	HA3	H	4.271	0.020	2
62	1	7	GLY	CA	C	46.195	0.400	1
63	1	7	GLY	N	N	113.396	0.400	1
64	1	8	LYS	H	H	7.974	0.020	1
65	1	8	LYS	HA	H	3.931	0.020	1
66	1	8	LYS	HB2	H	1.219	0.020	2
67	1	8	LYS	HB3	H	1.557	0.020	2
68	1	8	LYS	HG2	H	0.971	0.020	2
69	1	8	LYS	HG3	H	1.364	0.020	2
70	1	8	LYS	HD2	H	1.368	0.020	2
71	1	8	LYS	HD3	H	1.510	0.020	2
72	1	8	LYS	HE2	H	2.863	0.020	2
73	1	8	LYS	HE3	H	2.975	0.020	2
74	1	8	LYS	CA	C	58.558	0.400	1
75	1	8	LYS	CB	C	33.792	0.400	1
76	1	8	LYS	CG	C	25.915	0.400	1
77	1	8	LYS	CD	C	29.298	0.400	1
78	1	8	LYS	CE	C	42.105	0.400	1
79	1	8	LYS	N	N	123.712	0.400	1
80	1	9	ALA	H	H	7.967	0.020	1
81	1	9	ALA	HA	H	4.977	0.020	1
82	1	9	ALA	HB1	H	1.260	0.020	1
83	1	9	ALA	HB2	H	1.260	0.020	1
84	1	9	ALA	HB3	H	1.260	0.020	1
85	1	9	ALA	CA	C	51.009	0.400	1
86	1	9	ALA	CB	C	21.137	0.400	1
87	1	9	ALA	N	N	125.357	0.400	1
88	1	10	PHE	H	H	8.613	0.020	1
89	1	10	PHE	HA	H	4.759	0.020	1
90	1	10	PHE	HB2	H	2.484	0.020	2
91	1	10	PHE	HB3	H	3.397	0.020	2
92	1	10	PHE	HD1	H	6.848	0.020	1
93	1	10	PHE	HD2	H	7.216	0.020	1
94	1	10	PHE	HE1	H	6.854	0.020	1
95	1	10	PHE	HE2	H	6.869	0.020	1
96	1	10	PHE	HZ	H	6.850	0.020	1
97	1	10	PHE	CA	C	57.285	0.400	1
98	1	10	PHE	CB	C	44.022	0.400	1
99	1	10	PHE	CD1	C	132.282	0.400	3
100	1	10	PHE	CE1	C	130.999	0.400	3
101	1	10	PHE	CZ	C	129.793	0.400	1
102	1	10	PHE	N	N	116.516	0.400	1
103	1	11	VAL	H	H	9.002	0.020	1
104	1	11	VAL	HA	H	4.119	0.020	1
105	1	11	VAL	HB	H	2.235	0.020	1
106	1	11	VAL	HG11	H	1.104	0.020	2
107	1	11	VAL	HG12	H	1.104	0.020	2
108	1	11	VAL	HG13	H	1.104	0.020	2
109	1	11	VAL	HG21	H	1.082	0.020	2
110	1	11	VAL	HG22	H	1.082	0.020	2
111	1	11	VAL	HG23	H	1.082	0.020	2
112	1	11	VAL	CA	C	64.782	0.400	1
113	1	11	VAL	CB	C	32.612	0.400	1
114	1	11	VAL	CG1	C	21.842	0.400	2
115	1	11	VAL	CG2	C	21.386	0.400	2
116	1	11	VAL	N	N	123.458	0.400	1
117	1	12	LEU	H	H	8.005	0.020	1
118	1	12	LEU	HA	H	4.947	0.020	1
119	1	12	LEU	HB2	H	1.336	0.020	2
120	1	12	LEU	HB3	H	1.424	0.020	2
121	1	12	LEU	HD11	H	0.953	0.020	2
122	1	12	LEU	HD12	H	0.953	0.020	2
123	1	12	LEU	HD13	H	0.953	0.020	2
124	1	12	LEU	HD21	H	0.740	0.020	2
125	1	12	LEU	HD22	H	0.740	0.020	2
126	1	12	LEU	HD23	H	0.740	0.020	2
127	1	12	LEU	CA	C	53.075	0.400	1
128	1	12	LEU	CB	C	43.395	0.400	1
129	1	12	LEU	CG	C	26.720	0.400	1
130	1	12	LEU	CD1	C	22.975	0.400	2
131	1	12	LEU	CD2	C	25.091	0.400	2
132	1	12	LEU	N	N	117.992	0.400	1
133	1	13	SER	H	H	8.936	0.020	1
134	1	13	SER	HA	H	4.773	0.020	1
135	1	13	SER	HB2	H	3.453	0.020	2
136	1	13	SER	HB3	H	4.138	0.020	2
137	1	13	SER	CA	C	58.925	0.400	1
138	1	13	SER	CB	C	63.986	0.400	1
139	1	13	SER	N	N	122.272	0.400	1
140	1	14	ARG	H	H	8.570	0.020	1
141	1	14	ARG	HA	H	4.142	0.020	1
142	1	14	ARG	HB2	H	1.660	0.020	2
143	1	14	ARG	HB3	H	1.882	0.020	2
144	1	14	ARG	HG2	H	1.465	0.020	2
145	1	14	ARG	HG3	H	1.471	0.020	2
146	1	14	ARG	HD2	H	3.180	0.020	2
147	1	14	ARG	HD3	H	3.181	0.020	2
148	1	14	ARG	HE	H	7.111	0.020	1
149	1	14	ARG	CA	C	58.877	0.400	1
150	1	14	ARG	CB	C	29.466	0.400	1
151	1	14	ARG	CG	C	26.977	0.400	1
152	1	14	ARG	CD	C	43.435	0.400	1
153	1	14	ARG	N	N	119.165	0.400	1
154	1	15	HIS	H	H	6.834	0.020	1
155	1	15	HIS	HA	H	4.524	0.020	1
156	1	15	HIS	HB2	H	3.292	0.020	2
157	1	15	HIS	HB3	H	3.516	0.020	2
158	1	15	HIS	HD1	H	8.000	0.020	1
159	1	15	HIS	HD2	H	7.641	0.020	1
160	1	15	HIS	HE1	H	6.845	0.020	1
161	1	15	HIS	CA	C	56.957	0.400	1
162	1	15	HIS	CB	C	31.015	0.400	1
163	1	15	HIS	CD2	C	123.414	0.400	1
164	1	15	HIS	CE1	C	136.766	0.400	1
165	1	15	HIS	N	N	117.908	0.400	1
166	1	15	HIS	ND1	N	117.978	0.400	1
167	1	16	LEU	H	H	7.111	0.020	1
168	1	16	LEU	HA	H	3.084	0.020	1
169	1	16	LEU	HB2	H	1.102	0.020	2
170	1	16	LEU	HB3	H	1.902	0.020	2
171	1	16	LEU	HD11	H	0.987	0.020	2
172	1	16	LEU	HD12	H	0.987	0.020	2
173	1	16	LEU	HD13	H	0.987	0.020	2
174	1	16	LEU	HD21	H	0.969	0.020	2
175	1	16	LEU	HD22	H	0.969	0.020	2
176	1	16	LEU	HD23	H	0.969	0.020	2
177	1	16	LEU	CA	C	57.776	0.400	1
178	1	16	LEU	CB	C	40.103	0.400	1
179	1	16	LEU	CG	C	27.364	0.400	1
180	1	16	LEU	CD1	C	22.693	0.400	2
181	1	16	LEU	CD2	C	26.423	0.400	2
182	1	16	LEU	N	N	122.766	0.400	1
183	1	17	ASN	H	H	8.243	0.020	1
184	1	17	ASN	HA	H	4.300	0.020	1
185	1	17	ASN	HB2	H	2.734	0.020	2
186	1	17	ASN	HB3	H	2.738	0.020	2
187	1	17	ASN	HD21	H	6.826	0.020	2
188	1	17	ASN	HD22	H	7.449	0.020	2
189	1	17	ASN	CA	C	56.571	0.400	1
190	1	17	ASN	CB	C	37.832	0.400	1
191	1	17	ASN	N	N	116.603	0.400	1
192	1	17	ASN	ND2	N	111.984	0.400	1
193	1	18	ARG	H	H	7.427	0.020	1
194	1	18	ARG	HA	H	4.056	0.020	1
195	1	18	ARG	HB2	H	1.918	0.020	2
196	1	18	ARG	HB3	H	1.927	0.020	2
197	1	18	ARG	HG2	H	1.692	0.020	2
198	1	18	ARG	HG3	H	1.795	0.020	2
199	1	18	ARG	HD2	H	3.239	0.020	2
200	1	18	ARG	HD3	H	3.240	0.020	2
201	1	18	ARG	HE	H	7.430	0.020	1
202	1	18	ARG	CA	C	58.906	0.400	1
203	1	18	ARG	CB	C	30.177	0.400	1
204	1	18	ARG	CG	C	27.192	0.400	1
205	1	18	ARG	CD	C	43.493	0.400	1
206	1	18	ARG	N	N	118.460	0.400	1
207	1	19	HIS	H	H	7.632	0.020	1
208	1	19	HIS	HA	H	4.267	0.020	1
209	1	19	HIS	HB2	H	2.959	0.020	2
210	1	19	HIS	HB3	H	3.229	0.020	2
211	1	19	HIS	HD1	H	6.840	0.020	1
212	1	19	HIS	HD2	H	7.005	0.020	1
213	1	19	HIS	HE1	H	7.982	0.020	1
214	1	19	HIS	CA	C	59.083	0.400	1
215	1	19	HIS	CB	C	28.558	0.400	1
216	1	19	HIS	CD2	C	127.234	0.400	1
217	1	19	HIS	CE1	C	140.198	0.400	1
218	1	19	HIS	N	N	120.142	0.400	1
219	1	19	HIS	ND1	N	117.936	0.400	1
220	1	20	LEU	H	H	8.200	0.020	1
221	1	20	LEU	HA	H	3.818	0.020	1
222	1	20	LEU	HB2	H	1.550	0.020	2
223	1	20	LEU	HB3	H	1.915	0.020	2
224	1	20	LEU	HG	H	2.066	0.020	1
225	1	20	LEU	HD11	H	1.034	0.020	2
226	1	20	LEU	HD12	H	1.034	0.020	2
227	1	20	LEU	HD13	H	1.034	0.020	2
228	1	20	LEU	HD21	H	1.292	0.020	2
229	1	20	LEU	HD22	H	1.292	0.020	2
230	1	20	LEU	HD23	H	1.292	0.020	2
231	1	20	LEU	CA	C	58.163	0.400	1
232	1	20	LEU	CB	C	42.049	0.400	1
233	1	20	LEU	CG	C	27.039	0.400	1
234	1	20	LEU	CD1	C	25.530	0.400	2
235	1	20	LEU	CD2	C	24.655	0.400	2
236	1	20	LEU	N	N	116.808	0.400	1
237	1	21	ARG	H	H	7.106	0.020	1
238	1	21	ARG	HA	H	3.994	0.020	1
239	1	21	ARG	HB2	H	1.878	0.020	2
240	1	21	ARG	HB3	H	1.916	0.020	2
241	1	21	ARG	HG2	H	1.797	0.020	2
242	1	21	ARG	HG3	H	2.070	0.020	2
243	1	21	ARG	HD2	H	3.240	0.020	2
244	1	21	ARG	HD3	H	3.238	0.020	2
245	1	21	ARG	HE	H	7.111	0.020	1
246	1	21	ARG	CA	C	58.694	0.400	1
247	1	21	ARG	CB	C	30.047	0.400	1
248	1	21	ARG	CG	C	27.241	0.400	1
249	1	21	ARG	CD	C	43.509	0.400	1
250	1	21	ARG	N	N	116.353	0.400	1
251	1	22	VAL	H	H	7.982	0.020	1
252	1	22	VAL	HA	H	3.923	0.020	1
253	1	22	VAL	HB	H	1.980	0.020	1
254	1	22	VAL	HG11	H	0.478	0.020	2
255	1	22	VAL	HG12	H	0.478	0.020	2
256	1	22	VAL	HG13	H	0.478	0.020	2
257	1	22	VAL	HG21	H	0.708	0.020	2
258	1	22	VAL	HG22	H	0.708	0.020	2
259	1	22	VAL	HG23	H	0.708	0.020	2
260	1	22	VAL	CA	C	63.950	0.400	1
261	1	22	VAL	CB	C	30.986	0.400	1
262	1	22	VAL	CG1	C	19.161	0.400	2
263	1	22	VAL	CG2	C	19.916	0.400	2
264	1	22	VAL	N	N	115.932	0.400	1
265	1	23	HIS	H	H	7.109	0.020	1
266	1	23	HIS	HA	H	4.879	0.020	1
267	1	23	HIS	HB2	H	3.154	0.020	2
268	1	23	HIS	HB3	H	3.415	0.020	2
269	1	23	HIS	HD1	H	8.462	0.020	1
270	1	23	HIS	HD2	H	6.699	0.020	1
271	1	23	HIS	HE1	H	8.111	0.020	1
272	1	23	HIS	CA	C	54.605	0.400	1
273	1	23	HIS	CB	C	28.721	0.400	1
274	1	23	HIS	CD2	C	126.810	0.400	1
275	1	23	HIS	CE1	C	139.785	0.400	1
276	1	23	HIS	N	N	116.390	0.400	1
277	1	23	HIS	ND1	N	116.923	0.400	1
278	1	24	ARG	H	H	7.604	0.020	1
279	1	24	ARG	HA	H	4.377	0.020	1
280	1	24	ARG	HB2	H	1.826	0.020	2
281	1	24	ARG	HB3	H	1.824	0.020	2
282	1	24	ARG	HG2	H	1.695	0.020	2
283	1	24	ARG	HG3	H	1.918	0.020	2
284	1	24	ARG	HD2	H	3.233	0.020	2
285	1	24	ARG	HD3	H	3.234	0.020	2
286	1	24	ARG	HE	H	6.705	0.020	1
287	1	24	ARG	CA	C	56.205	0.400	1
288	1	24	ARG	CB	C	30.841	0.400	1
289	1	24	ARG	CG	C	27.195	0.400	1
290	1	24	ARG	CD	C	43.505	0.400	1
291	1	24	ARG	N	N	120.064	0.400	1
292	1	25	ARG	H	H	8.300	0.020	1
293	1	25	ARG	HA	H	4.378	0.020	1
294	1	25	ARG	HB2	H	1.827	0.020	2
295	1	25	ARG	HB3	H	1.914	0.020	2
296	1	25	ARG	HG2	H	1.684	0.020	2
297	1	25	ARG	HG3	H	1.686	0.020	2
298	1	25	ARG	HD2	H	3.234	0.020	2
299	1	25	ARG	HD3	H	3.235	0.020	2
300	1	25	ARG	HE	H	7.113	0.020	1
301	1	25	ARG	CA	C	56.170	0.400	1
302	1	25	ARG	CB	C	30.836	0.400	1
303	1	25	ARG	CG	C	27.165	0.400	1
304	1	25	ARG	CD	C	43.487	0.400	1
305	1	25	ARG	N	N	122.413	0.400	1
306	1	26	ALA	H	H	8.407	0.020	1
307	1	26	ALA	HA	H	4.427	0.020	1
308	1	26	ALA	HB1	H	1.456	0.020	1
309	1	26	ALA	HB2	H	1.456	0.020	1
310	1	26	ALA	HB3	H	1.456	0.020	1
311	1	26	ALA	CA	C	52.578	0.400	1
312	1	26	ALA	CB	C	19.443	0.400	1
313	1	26	ALA	N	N	126.202	0.400	1
314	1	27	THR	H	H	7.746	0.020	1
315	1	27	THR	HA	H	4.156	0.020	1
316	1	27	THR	HB	H	4.261	0.020	1
317	1	27	THR	HG21	H	1.191	0.020	1
318	1	27	THR	HG22	H	1.191	0.020	1
319	1	27	THR	HG23	H	1.191	0.020	1
320	1	27	THR	CA	C	63.139	0.400	1
321	1	27	THR	CB	C	70.838	0.400	1
322	1	27	THR	CG2	C	22.000	0.400	1
323	1	27	THR	N	N	118.576	0.400	1

Release date

2024-04-08

Related entities 1. Designed Zinc finger protein 5.3, entity 1, : 1 : 1398 entities Detail

Interaction partners 1. Designed Zinc finger protein 5.3, entity 1, : 15 interactors Detail

Experiments performed 8 experiments Detail

Chemical shift validation 4 contents Detail