BMRB: 31177

Covalent Complex Between Parkin Catalytic (Rcat) Domain and Ubiquitin

Authors

Connelly, E.M., Rintala-Dempsey, A.C., Gundogdu, M., Freeman, E.A., Koszela, J., Aguirre, J.D., Zhu, G., Kamarainen, O., Tadayon, R., Walden, H., Shaw, G.S.

Assembly

E3 ubiquitin-protein ligase parkin (E.C.2.3.2.31), Ubiquitin

Entity

1. E3 ubiquitin-protein ligase parkin (E.C.2.3.2.31), Ubiquitin, entity 1 (polymer), 73 monomers, 8269.334 Da Detail

TVDPNRAAEA RWDEASNVTI KVSTKPCPKC RTPTERDGGC MHMVCTRAGC GFEWCWVCQT EWTRDCMGAH WFG

2. E3 ubiquitin-protein ligase parkin (E.C.2.3.2.31), Ubiquitin, entity 2 (polymer), 75 monomers, 8507.679 Da Detail

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRG

3. E3 ubiquitin-protein ligase parkin (E.C.2.3.2.31), Ubiquitin, entity ZN (non-polymer), 65.409 × 2 Da
4. E3 ubiquitin-protein ligase parkin (E.C.2.3.2.31), Ubiquitin, entity 4LJ (non-polymer), 138.006 Da

Total weight

17045.836 Da

Max. entity weight

8507.679 Da

Source organism

Drosophila melanogaster , Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chemical Shifts 1

¹H: 60, ¹³C: 125, ¹⁵N: 60 assignments Detail

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• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

ID	Entity ID	Seq ID	Comp ID	Atom ID	Atom type	Val	Val err	Ambig. code
1	1	2	VAL	CA	C	62.1042	0.0000	1
2	1	3	ASP	H	H	8.5316	0.0000	1
3	1	3	ASP	CA	C	51.9199	0.0000	1
4	1	3	ASP	N	N	126.5359	0.0000	1
5	1	4	PRO	CA	C	63.9186	0.0000	1
6	1	4	PRO	CB	C	31.2086	0.0000	1
7	1	5	ASN	H	H	8.5038	0.0000	1
8	1	5	ASN	CA	C	53.9681	0.0000	1
9	1	5	ASN	CB	C	37.6397	0.0000	1
10	1	5	ASN	N	N	117.2551	0.0000	1
11	1	6	ARG	H	H	7.8761	0.0000	1
12	1	6	ARG	CA	C	56.4997	0.0000	1
13	1	6	ARG	CB	C	29.5964	0.0000	1
14	1	6	ARG	N	N	120.8663	0.0000	1
15	1	7	ALA	H	H	8.1669	0.0000	1
16	1	7	ALA	CA	C	53.3015	0.0000	1
17	1	7	ALA	CB	C	18.0410	0.0000	1
18	1	7	ALA	N	N	124.6472	0.0000	1
19	1	8	ALA	H	H	8.1594	0.0000	1
20	1	8	ALA	CA	C	53.1899	0.0000	1
21	1	8	ALA	CB	C	18.1298	0.0000	1
22	1	8	ALA	N	N	122.5732	0.0000	1
23	1	9	GLU	H	H	8.1554	0.0000	1
24	1	9	GLU	CA	C	56.9317	0.0000	1
25	1	9	GLU	CB	C	29.2736	0.0000	1
26	1	9	GLU	N	N	119.0486	0.0000	1
27	1	10	ALA	H	H	8.1989	0.0000	1
28	1	10	ALA	CA	C	53.0586	0.0000	1
29	1	10	ALA	CB	C	19.3067	0.0000	1
30	1	10	ALA	N	N	124.9307	0.0000	1
31	1	11	ARG	H	H	8.1013	0.0000	1
32	1	11	ARG	CA	C	55.1394	0.0000	1
33	1	11	ARG	CB	C	29.9412	0.0000	1
34	1	11	ARG	N	N	119.8699	0.0000	1
35	1	12	TRP	H	H	8.1563	0.0000	1
36	1	12	TRP	CA	C	57.7214	0.0000	1
37	1	12	TRP	CB	C	28.4673	0.0000	1
38	1	12	TRP	N	N	122.0962	0.0000	1
39	1	13	ASP	H	H	8.1855	0.0000	1
40	1	13	ASP	CA	C	54.8626	0.0000	1
41	1	13	ASP	CB	C	39.7316	0.0000	1
42	1	13	ASP	N	N	121.7060	0.0000	1
43	1	14	GLU	H	H	8.1290	0.0000	1
44	1	14	GLU	CA	C	57.6876	0.0000	1
45	1	14	GLU	CB	C	29.0757	0.0000	1
46	1	14	GLU	N	N	121.8075	0.0000	1
47	1	15	ALA	H	H	8.1569	0.0000	1
48	1	15	ALA	CA	C	53.1887	0.0000	1
49	1	15	ALA	CB	C	18.0177	0.0000	1
50	1	15	ALA	N	N	123.8425	0.0000	1
51	1	16	SER	H	H	8.0605	0.0000	1
52	1	16	SER	CA	C	58.7074	0.0000	1
53	1	16	SER	CB	C	62.7047	0.0000	1
54	1	16	SER	N	N	113.8987	0.0000	1
55	1	17	ASN	H	H	8.2586	0.0000	1
56	1	17	ASN	CA	C	53.5344	0.0000	1
57	1	17	ASN	CB	C	37.7532	0.0000	1
58	1	17	ASN	N	N	120.4936	0.0000	1
59	1	18	VAL	H	H	7.9551	0.0000	1
60	1	18	VAL	CA	C	62.5656	0.0000	1
61	1	18	VAL	CB	C	31.7844	0.0000	1
62	1	18	VAL	N	N	119.4545	0.0000	1
63	1	19	THR	H	H	8.2234	0.0000	1
64	1	19	THR	CA	C	62.2551	0.0000	1
65	1	19	THR	CB	C	68.8290	0.0000	1
66	1	19	THR	N	N	118.1989	0.0000	1
67	1	20	ILE	H	H	8.1390	0.0000	1
68	1	20	ILE	CA	C	61.0835	0.0000	1
69	1	20	ILE	CB	C	37.6432	0.0000	1
70	1	20	ILE	N	N	123.9943	0.0000	1
71	1	21	LYS	H	H	8.4018	0.0000	1
72	1	21	LYS	CA	C	56.1193	0.0000	1
73	1	21	LYS	CB	C	32.4479	0.0000	1
74	1	21	LYS	N	N	126.1829	0.0000	1
75	1	22	VAL	H	H	8.2812	0.0000	1
76	1	22	VAL	CA	C	62.2874	0.0000	1
77	1	22	VAL	CB	C	33.2392	0.0000	1
78	1	22	VAL	N	N	121.7534	0.0000	1
79	1	23	SER	CA	C	57.7211	0.0000	1
80	1	23	SER	CB	C	63.3278	0.0000	1
81	1	24	THR	H	H	7.9332	0.0000	1
82	1	24	THR	CA	C	60.6039	0.0000	1
83	1	24	THR	CB	C	71.2238	0.0000	1
84	1	24	THR	N	N	114.3298	0.0000	1
85	1	25	LYS	H	H	8.8920	0.0000	1
86	1	25	LYS	CA	C	54.5322	0.0000	1
87	1	25	LYS	CB	C	37.0751	0.0000	1
88	1	25	LYS	N	N	122.0197	0.0000	1
89	1	26	PRO	CA	C	60.2590	0.0000	1
90	1	26	PRO	CB	C	30.0868	0.0000	1
91	1	27	CYS	H	H	7.7529	0.0000	1
92	1	27	CYS	CA	C	54.7302	0.0000	1
93	1	27	CYS	CB	C	31.6587	0.0000	1
94	1	27	CYS	N	N	125.0419	0.0000	1
95	1	28	PRO	CA	C	64.1266	0.0000	1
96	1	28	PRO	CB	C	31.4594	0.0000	1
97	1	29	LYS	H	H	8.3205	0.0000	1
98	1	29	LYS	CA	C	57.8276	0.0000	1
99	1	29	LYS	CB	C	32.0663	0.0000	1
100	1	29	LYS	N	N	118.6086	0.0000	1
101	1	30	CYS	H	H	8.4105	0.0000	1
102	1	30	CYS	CA	C	59.4877	0.0000	1
103	1	30	CYS	CB	C	30.2266	0.0000	1
104	1	30	CYS	N	N	119.0736	0.0000	1
105	1	31	ARG	H	H	8.0881	0.0000	1
106	1	31	ARG	CA	C	56.8071	0.0000	1
107	1	31	ARG	CB	C	25.0445	0.0000	1
108	1	31	ARG	N	N	116.9081	0.0000	1
109	1	32	THR	H	H	8.6376	0.0000	1
110	1	32	THR	CA	C	63.3606	0.0000	1
111	1	32	THR	CB	C	69.0252	0.0000	1
112	1	32	THR	N	N	122.2319	0.0000	1
113	1	33	PRO	CA	C	64.2076	0.0000	1
114	1	33	PRO	CB	C	30.8490	0.0000	1
115	1	34	THR	H	H	9.0398	0.0000	1
116	1	34	THR	CA	C	62.4683	0.0000	1
117	1	34	THR	CB	C	70.2480	0.0000	1
118	1	34	THR	N	N	122.6189	0.0000	1
119	1	35	GLU	H	H	8.7336	0.0000	1
120	1	35	GLU	CA	C	55.1979	0.0000	1
121	1	35	GLU	CB	C	30.6721	0.0000	1
122	1	35	GLU	N	N	126.2278	0.0000	1
123	1	36	ARG	H	H	8.1740	0.0000	1
124	1	36	ARG	CA	C	52.2778	0.0000	1
125	1	36	ARG	CB	C	31.9053	0.0000	1
126	1	36	ARG	N	N	124.1264	0.0000	1
127	1	37	ASP	H	H	8.5462	0.0000	1
128	1	37	ASP	CA	C	51.9560	0.0000	1
129	1	37	ASP	CB	C	40.2530	0.0000	1
130	1	37	ASP	N	N	126.7400	0.0000	1
131	1	38	GLY	H	H	8.1086	0.0000	1
132	1	38	GLY	CA	C	45.3020	0.0000	1
133	1	38	GLY	N	N	111.4917	0.0000	1
134	1	39	GLY	H	H	8.4281	0.0000	1
135	1	39	GLY	CA	C	44.1181	0.0000	1
136	1	39	GLY	N	N	109.8927	0.0000	1
137	1	40	CYS	H	H	7.8178	0.0000	1
138	1	40	CYS	CA	C	59.5259	0.0000	1
139	1	40	CYS	CB	C	28.0091	0.0000	1
140	1	40	CYS	N	N	120.4854	0.0000	1
141	1	41	MET	CA	C	54.1776	0.0000	1
142	1	41	MET	CB	C	33.1009	0.0000	1
143	1	42	HIS	H	H	7.5602	0.0000	1
144	1	42	HIS	CA	C	53.8838	0.0000	1
145	1	42	HIS	CB	C	26.9363	0.0000	1
146	1	42	HIS	N	N	119.8436	0.0000	1
147	1	43	MET	H	H	9.1097	0.0000	1
148	1	43	MET	CA	C	52.7782	0.0000	1
149	1	43	MET	CB	C	28.1846	0.0000	1
150	1	43	MET	N	N	128.5579	0.0000	1
151	1	44	VAL	H	H	8.0485	0.0000	1
152	1	44	VAL	CA	C	60.5507	0.0000	1
153	1	44	VAL	CB	C	32.0303	0.0000	1
154	1	44	VAL	N	N	121.8106	0.0000	1
155	1	45	CYS	H	H	8.9974	0.0000	1
156	1	45	CYS	CA	C	60.7035	0.0000	1
157	1	45	CYS	CB	C	32.2870	0.0000	1
158	1	45	CYS	N	N	130.3033	0.0000	1
159	1	46	THR	H	H	8.5150	0.0000	1
160	1	46	THR	CA	C	61.5739	0.0000	1
161	1	46	THR	CB	C	68.3340	0.0000	1
162	1	46	THR	N	N	118.9500	0.0000	1
163	1	47	ARG	H	H	9.0302	0.0000	1
164	1	47	ARG	CA	C	57.2327	0.0000	1
165	1	47	ARG	CB	C	29.1060	0.0000	1
166	1	47	ARG	N	N	128.6300	0.0000	1
167	1	48	ALA	H	H	9.0024	0.0000	1
168	1	48	ALA	CA	C	54.2828	0.0000	1
169	1	48	ALA	CB	C	16.8758	0.0000	1
170	1	48	ALA	N	N	132.7264	0.0000	1
171	1	49	GLY	H	H	8.8326	0.0000	1
172	1	49	GLY	CA	C	45.6834	0.0000	1
173	1	49	GLY	N	N	111.4010	0.0000	1
174	1	50	CYS	H	H	8.0835	0.0000	1
175	1	50	CYS	CA	C	60.7366	0.0000	1
176	1	50	CYS	CB	C	30.2843	0.0000	1
177	1	50	CYS	N	N	123.1498	0.0000	1
178	1	51	GLY	H	H	7.6003	0.0000	1
179	1	51	GLY	CA	C	46.4160	0.0000	1
180	1	51	GLY	N	N	109.67	0.0000	1
181	1	52	PHE	H	H	8.8903	0.0000	1
182	1	52	PHE	CA	C	60.4754	0.0000	1
183	1	52	PHE	CB	C	37.8794	0.0000	1
184	1	52	PHE	N	N	124.7124	0.0000	1
185	1	53	GLU	H	H	7.7650	0.0000	1
186	1	53	GLU	CA	C	57.0388	0.0000	1
187	1	53	GLU	CB	C	30.1638	0.0000	1
188	1	53	GLU	N	N	125.6631	0.0000	1
189	1	54	TRP	H	H	9.0886	0.0000	1
190	1	54	TRP	CA	C	54.1482	0.0000	1
191	1	54	TRP	CB	C	31.9285	0.0000	1
192	1	54	TRP	N	N	120.9270	0.0000	1
193	1	55	CYS	H	H	8.8409	0.0000	1
194	1	55	CYS	CA	C	58.0688	0.0000	1
195	1	55	CYS	CB	C	31.7778	0.0000	1
196	1	55	CYS	N	N	120.8711	0.0000	1
197	1	56	TRP	H	H	9.8119	0.0000	1
198	1	56	TRP	CA	C	60.2925	0.0000	1
199	1	56	TRP	CB	C	30.2063	0.0000	1
200	1	56	TRP	N	N	131.0878	0.0000	1
201	1	57	VAL	H	H	8.6532	0.0000	1
202	1	57	VAL	CA	C	65.7248	0.0000	1
203	1	57	VAL	CB	C	33.3570	0.0000	1
204	1	57	VAL	N	N	118.4302	0.0000	1
205	1	59	GLN	H	H	6.8847	0.0000	1
206	1	59	GLN	CA	C	58.4757	0.0000	1
207	1	59	GLN	N	N	118.4633	0.0000	1
208	1	60	THR	H	H	7.0891	0.0000	1
209	1	60	THR	CA	C	59.2578	0.0000	1
210	1	60	THR	CB	C	71.3018	0.0000	1
211	1	60	THR	N	N	130.7824	0.0000	1
212	1	61	GLU	H	H	8.9044	0.0000	1
213	1	61	GLU	CA	C	56.9816	0.0000	1
214	1	61	GLU	CB	C	29.9431	0.0000	1
215	1	61	GLU	N	N	121.1851	0.0000	1
216	1	62	TRP	H	H	8.3988	0.0000	1
217	1	62	TRP	CA	C	58.5623	0.0000	1
218	1	62	TRP	N	N	126.8837	0.0000	1
219	1	63	THR	H	H	8.0614	0.0000	1
220	1	63	THR	CA	C	65.2502	0.0000	1
221	1	63	THR	N	N	117.3112	0.0000	1
222	1	64	ARG	H	H	8.5178	0.0000	1
223	1	64	ARG	CA	C	58.5380	0.0000	1
224	1	64	ARG	CB	C	28.6142	0.0000	1
225	1	64	ARG	N	N	118.9142	0.0000	1
226	1	65	ASP	H	H	7.7370	0.0000	1
227	1	65	ASP	CA	C	56.9229	0.0000	1
228	1	65	ASP	CB	C	39.4152	0.0000	1
229	1	65	ASP	N	N	119.5964	0.0000	1
230	1	66	CYS	H	H	6.9893	0.0000	1
231	1	66	CYS	CA	C	64.6662	0.0000	1
232	1	66	CYS	CB	C	29.1423	0.0000	1
233	1	66	CYS	N	N	120.6912	0.0000	1
234	1	67	MET	CA	C	58.0490	0.0000	1
235	1	68	GLY	H	H	9.1526	0.0000	1
236	1	68	GLY	CA	C	45.9046	0.0000	1
237	1	68	GLY	N	N	112.2792	0.0000	1
238	1	69	ALA	H	H	7.3059	0.0000	1
239	1	69	ALA	CA	C	53.5304	0.0000	1
240	1	69	ALA	CB	C	18.2644	0.0000	1
241	1	69	ALA	N	N	121.3170	0.0000	1
242	1	70	HIS	H	H	8.1825	0.0000	1
243	1	70	HIS	CA	C	58.2286	0.0000	1
244	1	70	HIS	CB	C	30.24	0.0000	1
245	1	70	HIS	N	N	115.3832	0.0000	1

Chemical Shifts 2

¹H: 57, ¹³C: 105, ¹⁵N: 57 assignments Detail

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ID	Entity ID	Seq ID	Comp ID	Atom ID	Atom type	Val	Val err	Ambig. code
246	2	1	MET	CA	C	54.6327	0.0000	1
247	2	1	MET	CB	C	33.2233	0.0000	1
248	2	2	GLN	H	H	8.9534	0.0000	1
249	2	2	GLN	CA	C	54.9365	0.0000	1
250	2	2	GLN	CB	C	30.5645	0.0000	1
251	2	2	GLN	N	N	123.0011	0.0000	1
252	2	3	ILE	H	H	8.3295	0.0000	1
253	2	3	ILE	CA	C	59.5998	0.0000	1
254	2	3	ILE	CB	C	42.1238	0.0000	1
255	2	3	ILE	N	N	115.2145	0.0000	1
256	2	4	PHE	H	H	8.6433	0.0000	1
257	2	4	PHE	CA	C	55.1507	0.0000	1
258	2	4	PHE	CB	C	41.5354	0.0000	1
259	2	4	PHE	N	N	118.7759	0.0000	1
260	2	5	VAL	H	H	9.3113	0.0000	1
261	2	5	VAL	CA	C	60.6321	0.0000	1
262	2	5	VAL	N	N	121.3094	0.0000	1
263	2	6	LYS	H	H	8.9860	0.0000	1
264	2	6	LYS	CA	C	54.7653	0.0000	1
265	2	6	LYS	CB	C	34.6272	0.0000	1
266	2	6	LYS	N	N	128.0508	0.0000	1
267	2	7	THR	H	H	8.7591	0.0000	1
268	2	7	THR	CA	C	60.6286	0.0000	1
269	2	7	THR	N	N	115.4485	0.0000	1
270	2	8	LEU	CB	C	41.9476	0.0000	1
271	2	9	THR	H	H	7.6493	0.0000	1
272	2	9	THR	CA	C	61.4826	0.0000	1
273	2	9	THR	CB	C	69.0853	0.0000	1
274	2	9	THR	N	N	105.7716	0.0000	1
275	2	10	GLY	H	H	7.8299	0.0000	1
276	2	10	GLY	CA	C	45.4336	0.0000	1
277	2	10	GLY	N	N	109.3562	0.0000	1
278	2	11	LYS	H	H	7.2840	0.0000	1
279	2	11	LYS	CA	C	56.3486	0.0000	1
280	2	11	LYS	CB	C	33.5813	0.0000	1
281	2	11	LYS	N	N	121.8452	0.0000	1
282	2	12	THR	H	H	8.6520	0.0000	1
283	2	12	THR	CA	C	62.3934	0.0000	1
284	2	12	THR	N	N	120.6377	0.0000	1
285	2	14	THR	H	H	8.7504	0.0000	1
286	2	14	THR	CA	C	62.2427	0.0000	1
287	2	14	THR	CB	C	72.0615	0.0000	1
288	2	14	THR	N	N	121.9011	0.0000	1
289	2	15	LEU	H	H	8.7642	0.0000	1
290	2	15	LEU	CA	C	52.8501	0.0000	1
291	2	15	LEU	CB	C	46.8715	0.0000	1
292	2	15	LEU	N	N	125.1076	0.0000	1
293	2	16	GLU	H	H	8.1526	0.0000	1
294	2	16	GLU	CA	C	54.8471	0.0000	1
295	2	16	GLU	CB	C	29.9509	0.0000	1
296	2	16	GLU	N	N	122.6366	0.0000	1
297	2	17	VAL	H	H	8.9478	0.0000	1
298	2	17	VAL	CA	C	58.4292	0.0000	1
299	2	17	VAL	CB	C	36.4733	0.0000	1
300	2	17	VAL	N	N	117.6430	0.0000	1
301	2	18	GLU	H	H	8.6886	0.0000	1
302	2	18	GLU	CA	C	52.8575	0.0000	1
303	2	18	GLU	CB	C	30.8915	0.0000	1
304	2	18	GLU	N	N	119.4305	0.0000	1
305	2	19	PRO	CA	C	65.3638	0.0000	1
306	2	20	SER	H	H	7.0471	0.0000	1
307	2	20	SER	CA	C	57.3258	0.0000	1
308	2	20	SER	CB	C	63.3927	0.0000	1
309	2	20	SER	N	N	103.5196	0.0000	1
310	2	21	ASP	H	H	8.0678	0.0000	1
311	2	21	ASP	CA	C	55.8612	0.0000	1
312	2	21	ASP	CB	C	40.9374	0.0000	1
313	2	21	ASP	N	N	123.9972	0.0000	1
314	2	22	THR	H	H	7.8836	0.0000	1
315	2	22	THR	CA	C	59.5892	0.0000	1
316	2	22	THR	CB	C	71.2175	0.0000	1
317	2	22	THR	N	N	109.0341	0.0000	1
318	2	23	ILE	H	H	8.5372	0.0000	1
319	2	23	ILE	CA	C	62.3687	0.0000	1
320	2	23	ILE	N	N	121.3571	0.0000	1
321	2	24	GLU	CA	C	53.1763	0.0000	1
322	2	25	ASN	H	H	7.9402	0.0000	1
323	2	25	ASN	CA	C	56.0195	0.0000	1
324	2	25	ASN	CB	C	38.4081	0.0000	1
325	2	25	ASN	N	N	121.4901	0.0000	1
326	2	26	VAL	H	H	8.1095	0.0000	1
327	2	26	VAL	CA	C	67.6743	0.0000	1
328	2	26	VAL	N	N	122.2572	0.0000	1
329	2	27	LYS	H	H	8.5752	0.0000	1
330	2	27	LYS	CA	C	59.3845	0.0000	1
331	2	27	LYS	N	N	119.0374	0.0000	1
332	2	28	ALA	H	H	7.9818	0.0000	1
333	2	28	ALA	CA	C	55.3829	0.0000	1
334	2	28	ALA	CB	C	18.2538	0.0000	1
335	2	28	ALA	N	N	123.5201	0.0000	1
336	2	29	LYS	H	H	7.8744	0.0000	1
337	2	29	LYS	CA	C	59.8159	0.0000	1
338	2	29	LYS	CB	C	33.4127	0.0000	1
339	2	29	LYS	N	N	120.3069	0.0000	1
340	2	30	ILE	H	H	8.2932	0.0000	1
341	2	30	ILE	CA	C	66.0590	0.0000	1
342	2	30	ILE	CB	C	36.7614	0.0000	1
343	2	30	ILE	N	N	121.3982	0.0000	1
344	2	31	GLN	H	H	8.5678	0.0000	1
345	2	31	GLN	CA	C	60.0008	0.0000	1
346	2	31	GLN	N	N	123.5778	0.0000	1
347	2	32	ASP	H	H	8.0534	0.0000	1
348	2	32	ASP	CA	C	57.2659	0.0000	1
349	2	32	ASP	CB	C	41.1481	0.0000	1
350	2	32	ASP	N	N	119.8893	0.0000	1
351	2	33	LYS	H	H	7.4488	0.0000	1
352	2	33	LYS	CA	C	58.1449	0.0000	1
353	2	33	LYS	CB	C	34.0664	0.0000	1
354	2	33	LYS	N	N	115.6014	0.0000	1
355	2	34	GLU	H	H	8.7502	0.0000	1
356	2	34	GLU	CA	C	55.4531	0.0000	1
357	2	34	GLU	CB	C	33.2850	0.0000	1
358	2	34	GLU	N	N	114.4078	0.0000	1
359	2	35	GLY	H	H	8.5119	0.0000	1
360	2	35	GLY	CA	C	46.1348	0.0000	1
361	2	35	GLY	N	N	108.9081	0.0000	1
362	2	36	ILE	H	H	6.1743	0.0000	1
363	2	36	ILE	N	N	120.2604	0.0000	1
364	2	38	PRO	CA	C	66.2430	0.0000	1
365	2	38	PRO	CB	C	32.8858	0.0000	1
366	2	39	ASP	H	H	8.5405	0.0000	1
367	2	39	ASP	CA	C	55.8169	0.0000	1
368	2	39	ASP	CB	C	39.9696	0.0000	1
369	2	39	ASP	N	N	113.6654	0.0000	1
370	2	40	GLN	H	H	7.8093	0.0000	1
371	2	40	GLN	CA	C	55.5442	0.0000	1
372	2	40	GLN	CB	C	29.7391	0.0000	1
373	2	40	GLN	N	N	116.9485	0.0000	1
374	2	41	GLN	H	H	7.4518	0.0000	1
375	2	41	GLN	CA	C	56.5769	0.0000	1
376	2	41	GLN	N	N	118.1559	0.0000	1
377	2	44	ILE	H	H	9.1391	0.0000	1
378	2	44	ILE	N	N	122.5467	0.0000	1
379	2	46	ALA	H	H	8.9991	0.0000	1
380	2	46	ALA	CA	C	52.6219	0.0000	1
381	2	46	ALA	CB	C	17.1217	0.0000	1
382	2	46	ALA	N	N	101.0408	0.0000	1
383	2	47	GLY	H	H	8.1087	0.0000	1
384	2	47	GLY	CA	C	45.4812	0.0000	1
385	2	47	GLY	N	N	102.5877	0.0000	1
386	2	48	LYS	H	H	7.9935	0.0000	1
387	2	48	LYS	CA	C	54.7007	0.0000	1
388	2	48	LYS	CB	C	34.6272	0.0000	1
389	2	48	LYS	N	N	121.8950	0.0000	1
390	2	49	GLN	H	H	8.6652	0.0000	1
391	2	49	GLN	CA	C	55.8394	0.0000	1
392	2	49	GLN	N	N	122.8798	0.0000	1
393	2	50	LEU	CA	C	54.2543	0.0000	1
394	2	50	LEU	CB	C	41.5020	0.0000	1
395	2	51	GLU	H	H	8.4036	0.0000	1
396	2	51	GLU	CA	C	56.0133	0.0000	1
397	2	51	GLU	CB	C	31.8884	0.0000	1
398	2	51	GLU	N	N	123.2603	0.0000	1
399	2	52	ASP	H	H	8.1737	0.0000	1
400	2	52	ASP	CA	C	56.4298	0.0000	1
401	2	52	ASP	CB	C	40.8193	0.0000	1
402	2	52	ASP	N	N	120.5350	0.0000	1
403	2	53	GLY	CA	C	45.2543	0.0000	1
404	2	54	ARG	H	H	7.4770	0.0000	1
405	2	54	ARG	CA	C	54.2658	0.0000	1
406	2	54	ARG	CB	C	32.8440	0.0000	1
407	2	54	ARG	N	N	119.4864	0.0000	1
408	2	55	THR	H	H	8.8483	0.0000	1
409	2	55	THR	CA	C	59.6495	0.0000	1
410	2	55	THR	CB	C	72.2786	0.0000	1
411	2	55	THR	N	N	108.9577	0.0000	1
412	2	56	LEU	H	H	8.1639	0.0000	1
413	2	56	LEU	CA	C	58.8402	0.0000	1
414	2	56	LEU	CB	C	40.4296	0.0000	1
415	2	56	LEU	N	N	118.1324	0.0000	1
416	2	57	SER	H	H	8.4891	0.0000	1
417	2	57	SER	CA	C	60.9688	0.0000	1
418	2	57	SER	CB	C	62.2841	0.0000	1
419	2	57	SER	N	N	113.5360	0.0000	1
420	2	58	ASP	H	H	7.9483	0.0000	1
421	2	58	ASP	CA	C	57.4652	0.0000	1
422	2	58	ASP	CB	C	40.4691	0.0000	1
423	2	58	ASP	N	N	124.6195	0.0000	1
424	2	59	TYR	H	H	7.2668	0.0000	1
425	2	59	TYR	CA	C	58.2523	0.0000	1
426	2	59	TYR	CB	C	40.1485	0.0000	1
427	2	59	TYR	N	N	115.8364	0.0000	1
428	2	60	ASN	H	H	8.1631	0.0000	1
429	2	60	ASN	CA	C	54.1833	0.0000	1
430	2	60	ASN	CB	C	37.4462	0.0000	1
431	2	60	ASN	N	N	116.0278	0.0000	1
432	2	61	ILE	H	H	7.2566	0.0000	1
433	2	61	ILE	CA	C	62.5171	0.0000	1
434	2	61	ILE	CB	C	37.0230	0.0000	1
435	2	61	ILE	N	N	118.9947	0.0000	1
436	2	62	GLN	H	H	7.6346	0.0000	1
437	2	62	GLN	CA	C	53.6163	0.0000	1
438	2	62	GLN	CB	C	31.6965	0.0000	1
439	2	62	GLN	N	N	125.0547	0.0000	1
440	2	63	LYS	H	H	8.4980	0.0000	1
441	2	63	LYS	CA	C	57.8528	0.0000	1
442	2	63	LYS	CB	C	32.4479	0.0000	1
443	2	63	LYS	N	N	120.6708	0.0000	1
444	2	64	GLU	H	H	9.3390	0.0000	1
445	2	64	GLU	CA	C	58.0172	0.0000	1
446	2	64	GLU	CB	C	26.3957	0.0000	1
447	2	64	GLU	N	N	114.6743	0.0000	1
448	2	65	SER	H	H	7.6804	0.0000	1
449	2	65	SER	CA	C	60.9175	0.0000	1
450	2	65	SER	CB	C	64.8436	0.0000	1
451	2	65	SER	N	N	115.0850	0.0000	1
452	2	66	THR	H	H	8.7315	0.0000	1
453	2	66	THR	CA	C	62.5104	0.0000	1
454	2	66	THR	CB	C	70.1454	0.0000	1
455	2	66	THR	N	N	117.5911	0.0000	1
456	2	67	LEU	H	H	9.3895	0.0000	1
457	2	67	LEU	CA	C	53.9600	0.0000	1
458	2	67	LEU	CB	C	44.2867	0.0000	1
459	2	67	LEU	N	N	127.8377	0.0000	1
460	2	68	HIS	H	H	9.1965	0.0000	1
461	2	68	HIS	CA	C	56.0987	0.0000	1
462	2	68	HIS	N	N	119.5451	0.0000	1
463	2	69	LEU	H	H	8.3396	0.0000	1
464	2	69	LEU	N	N	123.8867	0.0000	1

Release date

2024-06-23

Citation

Capturing the catalytic intermediates of parkin ubiquitination
Connelly, E.M., Rintala-Dempsey, A.C., Gundogdu, M., Freeman, E.A., Koszela, J., Aguirre, J.D., Zhu, G., Kamarainen, O., Tadayon, R., Walden, H., Shaw, G.S.
Proc. Natl. Acad. Sci. U. S. A. (2024), 121, e2403114121-e2403114121, PubMed 39078678 , DOI 10.1073/pnas.2403114121 , Abstract

Related entities 1. E3 ubiquitin-protein ligase parkin (E.C.2.3.2.31), Ubiquitin, entity 1, : 1 : 2 : 51 entities Detail

Related entities 2. E3 ubiquitin-protein ligase parkin (E.C.2.3.2.31), Ubiquitin, entity 2, : 92 : 6 : 46 : 177 entities Detail

Experiments performed 13 experiments Detail

Chemical shift validation 7 contents Detail