BMRB: 3322

Characterisation of a cellulosome dockerin domain from the anaerobic fungus Piromyces equi

Authors

Raghothama, S., Eberhardt, R.Y., Simpson, P.J., Wigelsworth, D., White, P., Hazlewood, G.P., Nagy, T., Gilbert, H.J., Williamson, M.P.

Assembly

endoglucanase Cel45A

Entity

1. endoglucanase Cel45A (polymer, Thiol state: all disulfide bound), 52 monomers, 5658.857 Da Detail

GSCWAQSQGY NCCNNPSSTK VEYTDASGQW GVQNGQWCGI DYSYGQNQGN ES

Formula weight

5658.857 Da

Entity Connection

disulfide 2 Detail

---

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	0:CYS3:SG	1:CYS12:SG
2	disulfide	sing	0:CYS13:SG	1:CYS38:SG

Source organism

Piromyces sp. 'equi'

Exptl. method

NMR

Refine. method

DISTANCE GEOMETRY, SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.1 %, Completeness (bb): 98.8 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	15N
All	96.1 % (343 of 357)	96.9 % (281 of 290)	92.5 % (62 of 67)
Backbone	98.8 % (160 of 162)	99.1 % (110 of 111)	98.0 % (50 of 51)
Sidechain	93.8 % (183 of 195)	95.5 % (171 of 179)	75.0 % (12 of 16)
Aromatic	100.0 % (37 of 37)	100.0 % (34 of 34)	100.0 % (3 of 3)
Methyl	100.0 % (10 of 10)	100.0 % (10 of 10)

1. dockerin

GSCWAQSQGY NCCNNPSSTK VEYTDASGQW GVQNGQWCGI DYSYGQNQGN ES

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	dockerin	[U-15N]		1 mM

Hide sample codition

Protein Blocks Logo

Calculated from 1 models in PDB: 1E8P, Strand ID: A Detail

---

Release date

1995-07-30

Citation

Characterization of a cellulosome dockerin domain from the anaerobic fungus Piromyces equi
Raghothama, S., Eberhardt, R.Y., Simpson, P.J., Wigelsworth, D., White, P., Hazlewood, G.P., Nagy, T., Gilbert, H.J., Williamson, M.P.
Nat. Struct. Biol. (2001), 8, 775-778, PubMed 11524680 , DOI 10.1038/nsb0901-775 , Abstract

Related entities 1. endoglucanase Cel45A, : 1 : 1 : 3 entities Detail

Experiments performed 8 experiments Detail