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BMRB: 34012

5LCB

In situ atomic-resolution structure of the baseplate antenna complex in Chlorobaculum tepidum obtained combining solid-state NMR spectroscopy, cryo electron microscopy and polarization spectroscopy

Authors

Nielsen, J.T., Kulminskaya, N.V., Bjerring, M., Linnanto, J.M., Ratsep, M., Pedersen, M., Lambrev, P.H., Dorogi, M., Garab, G., Thomsen, K., Jegerschold, C., Frigaard, N.U., Lindahl, M., Nielsen, N.C.

Assembly

Bacteriochlorophyll c-binding protein

Entity

1. Bacteriochlorophyll c-binding protein, entity 1 (polymer, Thiol state: not present), 59 monomers, 6153.984 × 14 Da Detail

MSGGGVFTDI LAAAGRIFEV MVEGHWETVG MLFDSLGKGT MRINRNAYGS MGGGSLRGS

2. Bacteriochlorophyll c-binding protein, entity BCL (non-polymer), 911.504 × 14 Da

Total weight

98916.83 Da

Max. entity weight

6153.984 Da

Source organism

Chlorobaculum tepidum TLS

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 74.6 %, Completeness: 64.0 %, Completeness (bb): 74.0 % Detail

Polymer type: polypeptide(L)

	Total	¹³C	¹⁵N
All	64.0 % (197 of 308)	62.2 % (153 of 246)	71.0 % (44 of 62)
Backbone	74.0 % (165 of 223)	73.8 % (121 of 164)	74.6 % (44 of 59)
Sidechain	50.4 % (66 of 131)	51.6 % (66 of 128)	0.0 % (0 of 3)
Aromatic	0.0 % (0 of 27)	0.0 % (0 of 26)	0.0 % (0 of 1)
Methyl	72.4 % (21 of 29)	72.4 % (21 of 29)

1. entity 1

MSGGGVFTDI LAAAGRIFEV MVEGHWETVG MLFDSLGKGT MRINRNAYGS MGGGSLRGS

Show sample condition

Sample

Solvent system solid state, lyophylized, Pressure 1 atm, Temperature 280 K, pH 7.0, Details 10.0 % [U-13C; U-15N] CsmA, 10.0 % [U-13C; U-15N] BACTERIOCHLOROPHYLL A, 35.0 % [U-13C] Carotenoids, 35.0 % [U-13C] Lipids, 10.0 % n.a. Magnesium ion, solid state, lyophylized

#	Name	Isotope labeling	Concentration
1	CsmA	[U-13C; U-15N]	10.0 %
2	BACTERIOCHLOROPHYLL A	[U-13C; U-15N]	10.0 %
3	Carotenoids	[U-13C]	35.0 %
4	Lipids	[U-13C]	35.0 %
5	Magnesium ion	n.a.	10.0 %

Protein Blocks Logo

Calculated from 1 models in PDB: 5LCB, Strand ID: A, B, C, D, E, F, G, H, I, J, K, L, M, N Detail

Release date

2016-12-06

Citation 1

In situ solid-state NMR spectroscopy of protein in heterogeneous membranes: the baseplate antenna complex of Chlorobaculum tepidum
Kulminskaya, N.V., Pedersen, M.O., Bjerring, M., Underhaug, J., Miller, M., Frigaard, N.U., Nielsen, J.T., Nielsen, N.C.
Angew. Chem Int Ed Engl. (2012), 51, 6891-6895, PubMed 22685072 , DOI 10.1002/anie.201201160 , Abstract

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Citation 2

In situ high-resolution structure of the baseplate antenna complex in Chlorobaculum tepidum
Nielsen, J.T., Kulminskaya, N.V., Bjerring, M., Linnanto, J.M., Ratsep, M., Pedersen, M., Lambrev, P.H., Dorogi, M., Garab, G., Thomsen, K., Jegerschold, C., Frigaard, N.U., Lindahl, M., Nielsen, N.C.
Nat. Commun. (2016), 7, 12454-12454, PubMed 27534696 , DOI 10.1038/ncomms12454 , Abstract

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