BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	70.2 % (1085 of 1545)	74.0 % (599 of 809)	63.6 % (382 of 601)	77.0 % (104 of 135)
Backbone	78.9 % (579 of 734)	91.1 % (226 of 248)	67.8 % (249 of 367)	87.4 % (104 of 119)
Sidechain	67.1 % (624 of 930)	66.5 % (373 of 561)	71.1 % (251 of 353)	0.0 % (0 of 16)
Aromatic	2.3 % (4 of 174)	4.6 % (4 of 87)	0.0 % (0 of 84)	0.0 % (0 of 3)
Methyl	95.2 % (120 of 126)	90.5 % (57 of 63)	100.0 % (63 of 63)

1. entity 1

GQELSQHERY VEQLKQALKT RGVKVKYADL LKFFDFVKDI CPWFPQEGTI DIKRWRRVGD CFQDYYNTFG PEKVPVIAFS YWNLIKELID KKEVNPQVMA AVAQTEEILK SNSQTDLEHH HHHH

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6, Details 1 mM [U-13C; U-15N] myristoylated retroviral matrix protein, 100 mM phosphate, 300 mM sodium choride, 5 mM DTT, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	DTT	none	5 mM
2	myristoylated retroviral matrix protein	[U-13C; U-15N]	1 mM
3	phosphate	none	100 mM
4	sodium choride	none	300 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6, Details 400 mM [U-15N] myristoylated retroviral matrix protein, 50 mM phosphate, 150 mM sodium choride, 5 mM DTT, 10 mg/mL Pf1 phage, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
5	DTT	none	5 mM
6	Pf1 phage	none	10 mg/mL
7	myristoylated retroviral matrix protein	[U-15N]	400 mM
8	phosphate	none	50 mM
9	sodium choride	none	150 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.15 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.15 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.14 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 30 models in PDB: 5LDL, Strand ID: A Detail

Release date

2016-07-20

Citation

Membrane Interactions of the Mason-Pfizer Monkey Virus Matrix Protein and Its Budding Deficient Mutants
Kroupa, T., Langerova, H., Dolezal, M., Prchal, J., Spiwok, V., Hunter, E., Rumlova, M., Hrabal, R., Ruml, T.
J. Mol. Biol. (2016), 428, 4708-4722, PubMed 27725181 , DOI 10.1016/j.jmb.2016.10.010 , Abstract

Related entities 1. myristoylated M-PMV matrix protein mutant, entity 1, : 1 : 2 : 26 entities Detail

Interaction partners 1. myristoylated M-PMV matrix protein mutant, entity 1, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 7 experiments Detail

1 3D CBCA(CO)NH

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6, Details 1 mM [U-13C; U-15N] myristoylated retroviral matrix protein, 100 mM phosphate, 300 mM sodium choride, 5 mM DTT, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	DTT	none	5 mM
2	myristoylated retroviral matrix protein	[U-13C; U-15N]	1 mM
3	phosphate	none	100 mM
4	sodium choride	none	300 mM

2 2D 1H-15N HSQC IPAP

Instrument

Bruker AvanceIII - 600 MHz

Sample

State anisotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6, Details 400 mM [U-15N] myristoylated retroviral matrix protein, 50 mM phosphate, 150 mM sodium choride, 5 mM DTT, 10 mg/mL Pf1 phage, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
5	DTT	none	5 mM
6	Pf1 phage	none	10 mg/mL
7	myristoylated retroviral matrix protein	[U-15N]	400 mM
8	phosphate	none	50 mM
9	sodium choride	none	150 mM

3 3D HNCACB

Instrument

Bruker AvanceIII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DTT	none	5 mM
2	myristoylated retroviral matrix protein	[U-13C; U-15N]	1 mM
3	phosphate	none	100 mM
4	sodium choride	none	300 mM

4 3D HCCH-TOCSY

Instrument

Bruker AvanceIII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DTT	none	5 mM
2	myristoylated retroviral matrix protein	[U-13C; U-15N]	1 mM
3	phosphate	none	100 mM
4	sodium choride	none	300 mM

5 3D 1H-13C NOESY

Instrument

Bruker AvanceIII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DTT	none	5 mM
2	myristoylated retroviral matrix protein	[U-13C; U-15N]	1 mM
3	phosphate	none	100 mM
4	sodium choride	none	300 mM

6 3D 1H-15N NOESY

Instrument

Bruker AvanceIII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DTT	none	5 mM
2	myristoylated retroviral matrix protein	[U-13C; U-15N]	1 mM
3	phosphate	none	100 mM
4	sodium choride	none	300 mM

7 3D HNCA

Instrument

Bruker AvanceIII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DTT	none	5 mM
2	myristoylated retroviral matrix protein	[U-13C; U-15N]	1 mM
3	phosphate	none	100 mM
4	sodium choride	none	300 mM

nullKeywords M-PMV, VIRAL PROTEIN, matrix protein, retrovirus

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Found 1 Document (1.057 seconds)

BMRB: 34015

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. myristoylated M-PMV matrix protein mutant, entity 1, : 1 : 2 : 26 entities Detail

Interaction partners 1. myristoylated M-PMV matrix protein mutant, entity 1, : 1 interactors Detail

Experiments performed 7 experiments Detail

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