BMRB: 34034

A two-quartet G-quadruplex formed by human telomere in KCl solution at neutral pH

Authors

Galer, P., Wang, B., Sket, P., Plavec, J.

Assembly

DNA (5'-D(*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*G)-3')

Entity

1. DNA (5'-D(*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*G)-3') (polymer, Thiol state: not present), 22 monomers, 7021.454 Da Detail

TAGGGTTAGG GTTAGGGTTA GG

Formula weight

7021.454 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 79.0 %, Completeness (bb): 78.6 % Detail

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Polymer type: polydeoxyribonucleotide

	Total	1H
All	79.0 % (162 of 205)	79.0 % (162 of 205)
Suger, PO4	78.6 % (121 of 154)	78.6 % (121 of 154)
Nucleobase	80.4 % (41 of 51)	80.4 % (41 of 51)
Aromatic	77.3 % (34 of 44)	77.3 % (34 of 44)
Methyl	100.0 % (7 of 7)	100.0 % (7 of 7)

1. DNA (5'-D(*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*G)-3')

TAGGGTTAGG GTTAGGGTTA GG

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 1 mM 8% 13C; 8% 15N DNA (5'-D(*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*G)-3'), 70 mM potassium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	DNA (5'-D(*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*G)-3')	8% 13C; 8% 15N		1 mM
2	potassium chloride	natural abundance		70 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 1 mM DNA (5'-D(*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*G)-3'), 70 mM potassium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
3	DNA (5'-D(*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*G)-3')	natural abundance		1 mM
4	potassium chloride	natural abundance		70 mM

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 1 mM DNA (5'-D(*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*G)-3'), 70 mM potassium chloride, 100% D2O

#	Name	Isotope labeling	Type	Concentration
5	DNA (5'-D(*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*G)-3')	natural abundance		1 mM
6	potassium chloride	natural abundance		70 mM

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Release date

2017-07-30

Citation

Reversible pH Switch of Two-Quartet G-Quadruplexes Formed by Human Telomere
Galer, P., Wang, B., Sket, P., Plavec, J.
Angew. Chem Int Ed Engl. (2016), 55, 1993-1997, PubMed 26836334 , DOI 10.1002/anie.201507569 , Abstract

Related entities 1. DNA (5'-D(*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*G)-3'), : 2 entities Detail

Experiments performed 9 experiments Detail