BMRBj - BMREntryMaster

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BMRB: 34067

5MF9

Solution structure of the RBM5 OCRE domain in complex with polyproline SmN peptide.

Authors

Mourao, A., Sattler, M., Bonnal, S., Komal, S., Warner, L., Bordonne, R., Valcarcel, J.

Assembly

RNA-binding protein 5, Survival motor neuron protein

Entity

1. RNA-binding protein 5, Survival motor neuron protein, entity 1 (polymer, Thiol state: not present), 64 monomers, 7434.765 Da Detail

GAMGTKYAVP DTSTYQYDES SGYYYDPTTG LYYDPNSQYY YNSLTQQYLY WDGEKETYVP AAES

2. RNA-binding protein 5, Survival motor neuron protein, entity 2 (polymer, Thiol state: not present), 11 monomers, 1134.354 Da Detail

GMRPPPPGIR G

Total weight

8569.119 Da

Max. entity weight

7434.765 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.7 %, Completeness: 88.2 %, Completeness (bb): 92.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	88.2 % (756 of 857)	89.9 % (399 of 444)	85.5 % (290 of 339)	90.5 % (67 of 74)
Backbone	92.4 % (401 of 434)	95.3 % (143 of 150)	91.2 % (198 of 217)	89.6 % (60 of 67)
Sidechain	86.1 % (422 of 490)	87.1 % (256 of 294)	84.1 % (159 of 189)	100.0 % (7 of 7)
Aromatic	62.9 % (78 of 124)	71.0 % (44 of 62)	54.1 % (33 of 61)	100.0 % (1 of 1)
Methyl	100.0 % (46 of 46)	100.0 % (23 of 23)	100.0 % (23 of 23)

1. entity 1

GAMGTKYAVP DTSTYQYDES SGYYYDPTTG LYYDPNSQYY YNSLTQQYLY WDGEKETYVP AAES

2. entity 2

GMRPPPPGIR G

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 6.5, Details 2 mM [U-99% 15N] 15N, 2 mM [U-99% 13C; U-99% 15N] 13C_15N, 2 mM [U-13C; U-15N] unlabel, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	entity_1	[U-99% 13C; U-99% 15N]	2 mM
2	entity_1	[U-99% 15N]	2 mM
3	entity_1	[U-13C; U-15N]	2 mM

Protein Blocks Logo

Calculated from 10 models in PDB: 5MF9, Strand ID: A, B Detail

Release date

2016-11-30

Citation

Structural basis for the recognition of spliceosomal SmN/B/B' proteins by the RBM5 OCRE domain in splicing regulation
Mourao, A., Bonnal, S., Komal, S., Warner, L., Bordonne, R., Valcarcel, J., Sattler, M.
Elife (2016), 5, 1-25, PubMed 27894420 , DOI 10.7554/eLife.14707 , Abstract

Related entities 1. RNA-binding protein 5, Survival motor neuron protein, entity 1, : 4 : 22 entities Detail

Related entities 2. RNA-binding protein 5, Survival motor neuron protein, entity 2, : 3 entities Detail

Interaction partners 1. RNA-binding protein 5, Survival motor neuron protein, entity 1, : 31 interactors Detail

More details for 31 interactors identified by 14 citations

Experiments performed 9 experiments Detail

1 3D 1H-13C NOESY aliphatic

Instrument

Bruker DRX900 - 900 MHz cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 6.5, Details 2 mM [U-99% 15N] 15N, 2 mM [U-99% 13C; U-99% 15N] 13C_15N, 2 mM [U-13C; U-15N] unlabel, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	entity_1	[U-99% 13C; U-99% 15N]	2 mM
2	entity_1	[U-99% 15N]	2 mM
3	entity_1	[U-13C; U-15N]	2 mM

2 3D 1H-13C HSQC aromatic

Instrument

Bruker DRX900 - 900 MHz cryoprobe

Sample

#	Name	Isotope labeling	Concentration
1	entity_1	[U-99% 13C; U-99% 15N]	2 mM
2	entity_1	[U-99% 15N]	2 mM
3	entity_1	[U-13C; U-15N]	2 mM

3 2D 1H-15N HSQC

Instrument

Bruker DRX500 - 500 MHz

Sample

#	Name	Isotope labeling	Concentration
1	entity_1	[U-99% 13C; U-99% 15N]	2 mM
2	entity_1	[U-99% 15N]	2 mM
3	entity_1	[U-13C; U-15N]	2 mM

4 2D 1H-1H NOESY

Instrument

Bruker DRX900 - 900 MHz cryoprobe