BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	94.6 % (694 of 734)	92.5 % (347 of 375)	96.2 % (281 of 292)	98.5 % (66 of 67)
Backbone	95.5 % (359 of 376)	94.6 % (122 of 129)	95.2 % (178 of 187)	98.3 % (59 of 60)
Sidechain	94.5 % (394 of 417)	91.5 % (225 of 246)	98.8 % (162 of 164)	100.0 % (7 of 7)
Aromatic	100.0 % (124 of 124)	100.0 % (62 of 62)	100.0 % (61 of 61)	100.0 % (1 of 1)
Methyl	83.3 % (35 of 42)	76.2 % (16 of 21)	90.5 % (19 of 21)

1. entity 1

GAMGTKYAVP DTSTYQYDES SGYYYDPTTG LYYDPNSQYY YNSLTQQYLY WDGEKETYVP AAES

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1 mM [U-100% 13C; U-100% 15N] ocre5, 20 mM sodium phosphate, 50 mM sodium chloride, 10 % D2O, 90 % H2O, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	D2O	natural abundance	10 %
2	H2O	natural abundance	90 %
3	ocre5	[U-100% 13C; U-100% 15N]	1 mM
4	sodium chloride	natural abundance	50 mM
5	sodium phosphate	natural abundance	20 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.23 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.23 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.09 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.23 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 5MFY, Strand ID: A Detail

Release date

2016-11-30

Citation

Structural basis for the recognition of spliceosomal SmN/B/B' proteins by the RBM5 OCRE domain in splicing regulation
Mourao, A., Bonnal, S., Soni, K., Warner, L., Bordonne, R., Valcarcel, J., Sattler, M.
Elife (2016), 5, PubMed 27894420 , DOI 10.7554/eLife.14707 , Abstract

Related entities 1. RNA-binding protein 5, : 1 : 1 : 4 : 23 entities Detail

Interaction partners 1. RNA-binding protein 5, : 31 interactors Detail

More details for 31 interactors identified by 14 citations

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1 mM [U-100% 13C; U-100% 15N] ocre5, 20 mM sodium phosphate, 50 mM sodium chloride, 10 % D2O, 90 % H2O, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	D2O	natural abundance	10 %
2	H2O	natural abundance	90 %
3	ocre5	[U-100% 13C; U-100% 15N]	1 mM
4	sodium chloride	natural abundance	50 mM
5	sodium phosphate	natural abundance	20 mM

2 3D CBCA(CO)NH

Instrument

Bruker AvanceIII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	D2O	natural abundance	10 %
2	H2O	natural abundance	90 %
3	ocre5	[U-100% 13C; U-100% 15N]	1 mM
4	sodium chloride	natural abundance	50 mM
5	sodium phosphate	natural abundance	20 mM

3 3D HNCACB

Instrument

Bruker AvanceIII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	D2O	natural abundance	10 %
2	H2O	natural abundance	90 %
3	ocre5	[U-100% 13C; U-100% 15N]	1 mM
4	sodium chloride	natural abundance	50 mM
5	sodium phosphate	natural abundance	20 mM

4 2D 1H-13C HSQC

Instrument

Bruker AvanceIII - 900 MHz

Sample

#	Name	Isotope labeling	Concentration
1	D2O	natural abundance	10 %
2	H2O	natural abundance	90 %
3	ocre5	[U-100% 13C; U-100% 15N]	1 mM
4	sodium chloride	natural abundance	50 mM
5	sodium phosphate	natural abundance	20 mM

5 2D 1H-13C HSQC aromatic

Instrument

Bruker AvanceIII - 900 MHz

Sample

#	Name	Isotope labeling	Concentration
1	D2O	natural abundance	10 %
2	H2O	natural abundance	90 %
3	ocre5	[U-100% 13C; U-100% 15N]	1 mM
4	sodium chloride	natural abundance	50 mM
5	sodium phosphate	natural abundance	20 mM

6 3D H(CCO)NH

Instrument

Bruker AvanceIII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	D2O	natural abundance	10 %
2	H2O	natural abundance	90 %
3	ocre5	[U-100% 13C; U-100% 15N]	1 mM
4	sodium chloride	natural abundance	50 mM
5	sodium phosphate	natural abundance	20 mM

7 3D HNCO

Instrument

Bruker AvanceIII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	D2O	natural abundance	10 %
2	H2O	natural abundance	90 %
3	ocre5	[U-100% 13C; U-100% 15N]	1 mM
4	sodium chloride	natural abundance	50 mM
5	sodium phosphate	natural abundance	20 mM

8 3D HNCA

Instrument

Bruker AvanceIII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	D2O	natural abundance	10 %
2	H2O	natural abundance	90 %
3	ocre5	[U-100% 13C; U-100% 15N]	1 mM
4	sodium chloride	natural abundance	50 mM
5	sodium phosphate	natural abundance	20 mM

9 3D C(CO)NH

Instrument

Bruker AvanceIII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	D2O	natural abundance	10 %
2	H2O	natural abundance	90 %
3	ocre5	[U-100% 13C; U-100% 15N]	1 mM
4	sodium chloride	natural abundance	50 mM
5	sodium phosphate	natural abundance	20 mM

10 HDCB Kay

Instrument

Bruker AvanceIII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	D2O	natural abundance	10 %
2	H2O	natural abundance	90 %
3	ocre5	[U-100% 13C; U-100% 15N]	1 mM
4	sodium chloride	natural abundance	50 mM
5	sodium phosphate	natural abundance	20 mM

11 HECB Kay

Instrument

Bruker AvanceIII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	D2O	natural abundance	10 %
2	H2O	natural abundance	90 %
3	ocre5	[U-100% 13C; U-100% 15N]	1 mM
4	sodium chloride	natural abundance	50 mM
5	sodium phosphate	natural abundance	20 mM

12 3D 1H-13C NOESY

Instrument

Bruker AvanceIII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	D2O	natural abundance	10 %
2	H2O	natural abundance	90 %
3	ocre5	[U-100% 13C; U-100% 15N]	1 mM
4	sodium chloride	natural abundance	50 mM
5	sodium phosphate	natural abundance	20 mM

13 3D 1H-15N NOESY

Instrument

Bruker AvanceIII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	D2O	natural abundance	10 %
2	H2O	natural abundance	90 %
3	ocre5	[U-100% 13C; U-100% 15N]	1 mM
4	sodium chloride	natural abundance	50 mM
5	sodium phosphate	natural abundance	20 mM

14 3D 1H-13C NOESY

Instrument

Bruker AvanceIII - 900 MHz

Sample

#	Name	Isotope labeling	Concentration
1	D2O	natural abundance	10 %
2	H2O	natural abundance	90 %
3	ocre5	[U-100% 13C; U-100% 15N]	1 mM
4	sodium chloride	natural abundance	50 mM
5	sodium phosphate	natural abundance	20 mM

NMR combined restraints 3 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints	combined_34068_5mfy.nef
Input source #2: Coordindates	5mfy.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

--------10--------20--------30--------40--------50--------60----
GAMGTKYAVPDTSTYQYDESSGYYYDPTTGLYYDPNSQYYYNSLTQQYLYWDGEKETYVPAAES
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
GAMGTKYAVPDTSTYQYDESSGYYYDPTTGLYYDPNSQYYYNSLTQQYLYWDGEKETYVPAAES

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	64	0	0	100.0

Content subtype: combined_34068_5mfy.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	704		100.0 (chain: A, length: 64)
1	Distance restraints	CNS/XPLOR_distance_constraints_2	Warning	1331 (1)	noe	95.3 (chain: A, length: 64)

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 64, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 357
  - ¹³C chemical shifts: 282
  - ¹⁵N chemical shifts: 65
- Completeness of assignments
  - Entity_assemble_ID: A
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	375	357	95.2
¹³C chemical shifts	292	282	96.6
¹⁵N chemical shifts	67	65	97.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	129	125	96.9
¹³C chemical shifts	128	120	93.8
¹⁵N chemical shifts	60	58	96.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	246	232	94.3
¹³C chemical shifts	164	162	98.8
¹⁵N chemical shifts	7	7	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	22	20	90.9
¹³C chemical shifts	22	20	90.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	62	62	100.0
¹³C chemical shifts	61	61	100.0
¹⁵N chemical shifts	1	1	100.0

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_2
- Status: Warning
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 64, Sequence coverage: 95.3%
- Total number of restraints: 1331
- Weight of restraints: 1.0 (1331)
- Potential type of restraints: square-well-parabolic (1331)
- Range of distance target values: 1.18, 2.75 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map

Keywords ocre, splicing

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Link destinations for BMRB, PDB and EMDB

Found 1 Document (2.419 seconds)

BMRB: 34068

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. RNA-binding protein 5, : 1 : 1 : 4 : 23 entities Detail

Interaction partners 1. RNA-binding protein 5, : 31 interactors Detail

Experiments performed 14 experiments Detail

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NMR combined restraints 3 contents Detail