Structure of the N-terminal domain of the Escherichia Coli ProQ RNA binding protein
MGSSHHHHHH SQDPMENQPK LNSSKEVIAF LAERFPHCFS AEGEARPLKI GIFQDLVDRV AGEMNLSKTQ LRSALRLYTS SWRYLYGVKP GATRVDLDGN PCGELDEQHV EHARKQLEEA KARVQAQRAE QQA
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 80.9 % (1246 of 1541) | 76.4 % (619 of 810) | 85.1 % (501 of 589) | 88.7 % (126 of 142) |
Backbone | 91.5 % (719 of 786) | 91.0 % (244 of 268) | 91.3 % (357 of 391) | 92.9 % (118 of 127) |
Sidechain | 73.1 % (643 of 880) | 69.2 % (375 of 542) | 80.5 % (260 of 323) | 53.3 % (8 of 15) |
Aromatic | 68.8 % (77 of 112) | 71.4 % (40 of 56) | 65.5 % (36 of 55) | 100.0 % (1 of 1) |
Methyl | 94.2 % (113 of 120) | 95.0 % (57 of 60) | 93.3 % (56 of 60) |
1. entity 1
MGSSHHHHHH SQDPMENQPK LNSSKEVIAF LAERFPHCFS AEGEARPLKI GIFQDLVDRV AGEMNLSKTQ LRSALRLYTS SWRYLYGVKP GATRVDLDGN PCGELDEQHV EHARKQLEEA KARVQAQRAE QQASolvent system 90% H2O/10% D2O, Pressure 1 (±0.01) atm, Temperature 298 (±0.1) K, pH 6.5 (±0.1), Details 20 mM sodium phosphate, 100 mM sodium chloride, 1 mM TCEP, 0.0025 % 3,3,3-trimethylsilylpropionate, 90% H2O/10% D2O.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | entity_1 | natural abundance | 0.0 ~ 0.0 mM | |
2 | sodium phosphate | natural abundance | 20 (±1.0) mM | |
3 | sodium chloride | natural abundance | 100 (±5.0) mM | |
4 | TCEP | natural abundance | 1 (±0.1) mM | |
5 | 3,3,3-trimethylsilylpropionate | natural abundance | 0.0025 (±5.0E-4) % |
Solvent system 90% H2O/10% D2O, Pressure 1 (±0.01) atm, Temperature 298 (±0.1) K, pH 6.5 (±0.1), Details 20 mM sodium phosphate, 100 mM sodium chloride, 1 mM TCEP, 0.0025 % 3,3,3-trimethylsilylpropionate, 90% H2O/10% D2O.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | entity_1 | natural abundance | 0.0 ~ 0.0 mM | |
7 | sodium phosphate | natural abundance | 20 (±1.0) mM | |
8 | sodium chloride | natural abundance | 100 (±5.0) mM | |
9 | TCEP | natural abundance | 1 (±0.1) mM | |
10 | 3,3,3-trimethylsilylpropionate | natural abundance | 0.0025 (±5.0E-4) % |