NMR Structural Characterisation of Pharmaceutically Relevant Proteins Obtained Through a Novel Recombinant Production: The Case of The Pulmonary Surfactant Polypeptide C Analogue rSP-C33Leu.
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 68.0 % (289 of 425) | 93.6 % (204 of 218) | 31.3 % (55 of 176) | 96.8 % (30 of 31) |
Backbone | 70.6 % (137 of 194) | 98.5 % (65 of 66) | 43.3 % (42 of 97) | 96.8 % (30 of 31) |
Sidechain | 61.5 % (161 of 262) | 91.4 % (139 of 152) | 20.0 % (22 of 110) | |
Aromatic | 50.0 % (2 of 4) | 100.0 % (2 of 2) | 0.0 % (0 of 2) | |
Methyl | 53.3 % (48 of 90) | 97.8 % (44 of 45) | 8.9 % (4 of 45) |
1. entity 1
IPSSPVHLKR LKLLLLLLLL ILLLILGALL LGLSolvent system CDCl3/CD3OD/0.1 M HCl 32:64:5 (v/v), Pressure 1 atm, Temperature 298 K, pH 1, Details 1.7 mM No rSP-C33Leu -RECOMBINANT PULMONARY SURFACTANT-ASSOCIATED POLYPEPTIDE C ANALOGUE-, CDCl3/CD3OD/0.1 M HCl 32:64:5 (v/v)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | entity_1 | natural abundance | 1.7 mM |