BMRB: 34118

An i-motif containing the neutral cytidine protonated analogue pseudoisocytidine

Authors

Mir, B., Soles, X., Gonzalez, C., Escaja, N.

Assembly

DNA (5'-D(*TP*(DCP)P*CP*GP*TP*TP*TP*CP*(PSC)P*GP*T)-3')

Entity

1. DNA (5'-D(*TP*(DCP)P*CP*GP*TP*TP*TP*CP*(PSC)P*GP*T)-3') (polymer, Thiol state: not present), 11 monomers, 3354.117 × 2 Da Detail

TCCGTTTCXG T

Total weight

6708.234 Da

Max. entity weight

3354.117 Da

Source organism

synthetic construct

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.9 %, Completeness: 54.5 %, Completeness (bb): 41.4 % Detail

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Polymer type: polydeoxyribonucleotide

	Total	1H
All	54.5 % (55 of 101)	54.5 % (55 of 101)
Suger, PO4	41.4 % (29 of 70)	41.4 % (29 of 70)
Nucleobase	83.9 % (26 of 31)	83.9 % (26 of 31)
Aromatic	75.0 % (15 of 20)	75.0 % (15 of 20)
Methyl	100.0 % (5 of 5)	100.0 % (5 of 5)

1. entity 1

TCCGTTTCXG T

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 278 K, pH 7, Details 0.8 mM DNA (5'-D(*TP*(DCP)P*CP*GP*TP*TP*TP*CP*(PSC)P*GP*T)-3'), 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	DNA (5'-D(*TP*(DCP)P*CP*GP*TP*TP*TP*CP*(PSC)P*GP*T)-3')	natural abundance		0.8 mM
2	NaCl	natural abundance		100 mM

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Release date

2017-06-06

Citation

The effect of the neutral cytidine protonated analogue pseudoisocytidine on the stability of i-motif structures
Mir, B., Soles, X., Gonzalez, C., Escaja, N.
Sci. Rep. (2017), 7, 2772-2772, PubMed 28584239 , DOI 10.1038/s41598-017-02723-y , Abstract

Experiments performed 1 experiments Detail